Stromal cells differentially regulate neutrophil and lymphocyte recruitment through the endothelium.
Bottom Line: Stromal fibroblasts modify the initial recruitment of leucocytes by endothelial cells (EC), but their effects on subsequent transendothelial migration remain unclear.Fibroblasts had little effect on tumour necrosis factor-α-induced transendothelial migration of neutrophils, but tended to increase the efficiency of migration away from the endothelium.To avoid contact, we cultured EC and fibroblasts on separate 3-μm pore filters one above the other.
Affiliation: Centre for Cardiovascular Sciences, School of Clinical and Experimental Medicine, Medical School, The University of Birmingham, Birmingham, UK. email@example.comShow MeSH
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Mentions: We examined the ability of fibroblasts to modify the adhesion and migration of lymphocytes through EC with or without TNF-α + IFN-γ. Under static conditions, in the absence of cytokine treatment, PBL and CD4+ or CD8+ T-cell subsets (enumerated separately by flow cytometry) tended to be bound less efficiently to EC co-cultured with fibroblasts compared with EC cultured alone (Fig. 3a). Few lymphocytes migrated through the unstimulated cultures, but again, fewer migrated through co-cultures than endothelial monocultures (Fig. 3b). Adhesion and transmigration were increased and less variable after cytokine treatment. Under these conditions, dermal fibroblasts significantly inhibited adhesion compared with EC cultured alone, and co-culture with either type of fibroblast markedly reduced transmigration (Fig. 3). Similar suppression of migration by fibroblasts was observed when TNF-α alone was used as the stimulus (data not shown).
Affiliation: Centre for Cardiovascular Sciences, School of Clinical and Experimental Medicine, Medical School, The University of Birmingham, Birmingham, UK. firstname.lastname@example.org