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Predominant porB1A and porB1B genotypes and correlation of gene mutations with drug resistance in Neisseria gonorrhoeae isolates in Eastern China.

Sun A, Fan X, Gu Y, Du P, Tang R, Mao Y, Lin X, Yan J - BMC Infect. Dis. (2010)

Bottom Line: Particularly, two novel chimeric serotypes, IB3/6-IB2 and IB2-IB4-IB2, were found in 77 and 8 porB1B+ isolates.All the replacement mutations at these sites in PorB1B were correlated with resistance and the deletion mutation showed the highest resistance.Multiple mutations in porB1B genes, including novel A121 and N122 deletions, are correlated with high levels of penicillin and tetracycline resistance.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Basic Medical Microbiology, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital of Zhejiang University, Zhejiang 310003, Hangzhou, China.

ABSTRACT

Background: Variations of porB1A and porB1B genes and their serotypes exist in Neisseria gonorrhoeae isolates from different geographical areas, and some site mutations in the porB1B gene correlate with drug resistance.

Methods: The β-lactamase production of N. gonorrhoeae isolates was determined by paper acidometric test and nitrocefin discs. The porB1A and porB1B genes of 315 non-penicillinase-producting N. gonorrhoeae (non-PPNG) strains were amplified by PCR for sequencing to determine serotypes and site mutations. A duplex PCR was designed to simultaneously detect both porB1A and porB1B genes. Penicillin and tetracycline resistance was assessed by an in vitro drug sensitivity test.

Results: Of the N. gonorrhoeae isolates, 31.1% tested positive for porB1A and 68.9% for porB1B genes. All the 98 porB1A+ isolates belonging to IA6 serotype with either no mutation at the 120 and 121 sites (88.8%) or a D120G (11.2%) mutation and were no resistance to both penicillin and tetracycline. Among the 217 porB1B+ isolates, 26.7%, 22.6% and 11.5% belonged to IB3, IB3/6 and IB4 serotypes, respectively. Particularly, two novel chimeric serotypes, IB3/6-IB2 and IB2-IB4-IB2, were found in 77 and 8 porB1B+ isolates. Two hundred and twelve (97.7%) of the porB1B+ isolates were presented G120 and/or A121 mutations with 163 (76.9%) at both sites. Interestingly, within the 77 porB1B+ isolates belonging to IB3/6-IB2 serotype, 15 were discovered to possess novel deletions at both A121 and N122 sites. All the replacement mutations at these sites in PorB1B were correlated with resistance and the deletion mutation showed the highest resistance.

Conclusion: N. gonorrhoeae isolates circulating in Eastern China include a sole PorB1A serotype (IA6) and five PorB1B serotypes. Multiple mutations in porB1B genes, including novel A121 and N122 deletions, are correlated with high levels of penicillin and tetracycline resistance.

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Related in: MedlinePlus

Amplicons of entire or partial porB1A and porB1B genes. Note: Lane M: DNA marker (BioColor); Lanes 1 and 2: amplicons of entire porB1A gene (981 bp) and porB1B gene (1044 bp), respectively; Lanes 3 and 4: amplicons of partial porB1A gene (520 bp) and partial porB1B gene (583 bp and 201 bp) by duplex PCR, respectively; Lane 5: blank control.
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Figure 1: Amplicons of entire or partial porB1A and porB1B genes. Note: Lane M: DNA marker (BioColor); Lanes 1 and 2: amplicons of entire porB1A gene (981 bp) and porB1B gene (1044 bp), respectively; Lanes 3 and 4: amplicons of partial porB1A gene (520 bp) and partial porB1B gene (583 bp and 201 bp) by duplex PCR, respectively; Lane 5: blank control.

Mentions: Of the 315 N. gonorrhoeae isolates tested, 31.1% (98/315) and 68.9% (217/315) were found by separate PCR to possess porB1A and porB1B genes, respectively. The duplex PCR established in this study accurately distinguished the porB1A and porB1B genes (Figure 1), and presented the same positive rates for porB1A (31.1%) and porB1B (68.9%) genes in the same gonococcal isolates.


Predominant porB1A and porB1B genotypes and correlation of gene mutations with drug resistance in Neisseria gonorrhoeae isolates in Eastern China.

Sun A, Fan X, Gu Y, Du P, Tang R, Mao Y, Lin X, Yan J - BMC Infect. Dis. (2010)

Amplicons of entire or partial porB1A and porB1B genes. Note: Lane M: DNA marker (BioColor); Lanes 1 and 2: amplicons of entire porB1A gene (981 bp) and porB1B gene (1044 bp), respectively; Lanes 3 and 4: amplicons of partial porB1A gene (520 bp) and partial porB1B gene (583 bp and 201 bp) by duplex PCR, respectively; Lane 5: blank control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2992536&req=5

Figure 1: Amplicons of entire or partial porB1A and porB1B genes. Note: Lane M: DNA marker (BioColor); Lanes 1 and 2: amplicons of entire porB1A gene (981 bp) and porB1B gene (1044 bp), respectively; Lanes 3 and 4: amplicons of partial porB1A gene (520 bp) and partial porB1B gene (583 bp and 201 bp) by duplex PCR, respectively; Lane 5: blank control.
Mentions: Of the 315 N. gonorrhoeae isolates tested, 31.1% (98/315) and 68.9% (217/315) were found by separate PCR to possess porB1A and porB1B genes, respectively. The duplex PCR established in this study accurately distinguished the porB1A and porB1B genes (Figure 1), and presented the same positive rates for porB1A (31.1%) and porB1B (68.9%) genes in the same gonococcal isolates.

Bottom Line: Particularly, two novel chimeric serotypes, IB3/6-IB2 and IB2-IB4-IB2, were found in 77 and 8 porB1B+ isolates.All the replacement mutations at these sites in PorB1B were correlated with resistance and the deletion mutation showed the highest resistance.Multiple mutations in porB1B genes, including novel A121 and N122 deletions, are correlated with high levels of penicillin and tetracycline resistance.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Basic Medical Microbiology, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital of Zhejiang University, Zhejiang 310003, Hangzhou, China.

ABSTRACT

Background: Variations of porB1A and porB1B genes and their serotypes exist in Neisseria gonorrhoeae isolates from different geographical areas, and some site mutations in the porB1B gene correlate with drug resistance.

Methods: The β-lactamase production of N. gonorrhoeae isolates was determined by paper acidometric test and nitrocefin discs. The porB1A and porB1B genes of 315 non-penicillinase-producting N. gonorrhoeae (non-PPNG) strains were amplified by PCR for sequencing to determine serotypes and site mutations. A duplex PCR was designed to simultaneously detect both porB1A and porB1B genes. Penicillin and tetracycline resistance was assessed by an in vitro drug sensitivity test.

Results: Of the N. gonorrhoeae isolates, 31.1% tested positive for porB1A and 68.9% for porB1B genes. All the 98 porB1A+ isolates belonging to IA6 serotype with either no mutation at the 120 and 121 sites (88.8%) or a D120G (11.2%) mutation and were no resistance to both penicillin and tetracycline. Among the 217 porB1B+ isolates, 26.7%, 22.6% and 11.5% belonged to IB3, IB3/6 and IB4 serotypes, respectively. Particularly, two novel chimeric serotypes, IB3/6-IB2 and IB2-IB4-IB2, were found in 77 and 8 porB1B+ isolates. Two hundred and twelve (97.7%) of the porB1B+ isolates were presented G120 and/or A121 mutations with 163 (76.9%) at both sites. Interestingly, within the 77 porB1B+ isolates belonging to IB3/6-IB2 serotype, 15 were discovered to possess novel deletions at both A121 and N122 sites. All the replacement mutations at these sites in PorB1B were correlated with resistance and the deletion mutation showed the highest resistance.

Conclusion: N. gonorrhoeae isolates circulating in Eastern China include a sole PorB1A serotype (IA6) and five PorB1B serotypes. Multiple mutations in porB1B genes, including novel A121 and N122 deletions, are correlated with high levels of penicillin and tetracycline resistance.

Show MeSH
Related in: MedlinePlus