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External irradiation models for intracranial 9L glioma studies.

Vinchon-Petit S, Jarnet D, Jadaud E, Feuvret L, Garcion E, Menei P - J. Exp. Clin. Cancer Res. (2010)

Bottom Line: Toxicities of reduced weight and alopecia were increased during the radiation period but no serious morbidity or mortality was observed.Moreover, abnormalities disappeared the week following the end of the therapeutic schedule.Delivering 18 Gy in 3 fractions of 6 Gy every 3 days, with mild anaesthesia, is safe, easy to reproduce and allows for standardisation in preclinical studies of different treatment regimens glioma rat model.

View Article: PubMed Central - HTML - PubMed

Affiliation: INSERM, U646, Université d'Angers, Angers, F-49100, France. skaya2@free.fr

ABSTRACT

Purpose: Radiotherapy has been shown to be an effective for the treatment human glioma and consists of 30 fractions of 2 Gy each for 6-7 weeks in the tumor volume with margins. However. in preclinical studies, many different radiation schedules are used. The main purpose of this work was to review the relevant literature and to propose an external whole-brain irradiation (WBI) protocol for a rat 9L glioma model.

Materials and methods: 9L cells were implanted in the striatum of twenty 344-Fisher rats to induce a brain tumor. On day 8, animals were randomized in two groups: an untreated group and an irradiated group with three fractions of 6 Gy at day 8, 11 and 14. Survival and toxicity were assessed.

Results: Irradiated rats had significantly a longer survival (p = 0.01). No deaths occurred due to the treatment. Toxicities of reduced weight and alopecia were increased during the radiation period but no serious morbidity or mortality was observed. Moreover, abnormalities disappeared the week following the end of the therapeutic schedule.

Conclusions: Delivering 18 Gy in 3 fractions of 6 Gy every 3 days, with mild anaesthesia, is safe, easy to reproduce and allows for standardisation in preclinical studies of different treatment regimens glioma rat model.

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Dose distribution in one hemibrain (A) and in the whole rat brain (B).
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Figure 6: Dose distribution in one hemibrain (A) and in the whole rat brain (B).

Mentions: Some investigators use Plexiglas stereotactic frames for rat positioning and treat just one hemi-brain. Previously, in our laboratory, we used a fractionated radiotherapy in one hemi-brain [6]. We found that the volume of interest is better covered when the whole brain is treated, as opposed to hemi-brain irradiation, due to the small size of a rat brain (figure 6). The Dose Volume histogram (DVH) obtained for these two treatment modalities are represented in figure 7.


External irradiation models for intracranial 9L glioma studies.

Vinchon-Petit S, Jarnet D, Jadaud E, Feuvret L, Garcion E, Menei P - J. Exp. Clin. Cancer Res. (2010)

Dose distribution in one hemibrain (A) and in the whole rat brain (B).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2992475&req=5

Figure 6: Dose distribution in one hemibrain (A) and in the whole rat brain (B).
Mentions: Some investigators use Plexiglas stereotactic frames for rat positioning and treat just one hemi-brain. Previously, in our laboratory, we used a fractionated radiotherapy in one hemi-brain [6]. We found that the volume of interest is better covered when the whole brain is treated, as opposed to hemi-brain irradiation, due to the small size of a rat brain (figure 6). The Dose Volume histogram (DVH) obtained for these two treatment modalities are represented in figure 7.

Bottom Line: Toxicities of reduced weight and alopecia were increased during the radiation period but no serious morbidity or mortality was observed.Moreover, abnormalities disappeared the week following the end of the therapeutic schedule.Delivering 18 Gy in 3 fractions of 6 Gy every 3 days, with mild anaesthesia, is safe, easy to reproduce and allows for standardisation in preclinical studies of different treatment regimens glioma rat model.

View Article: PubMed Central - HTML - PubMed

Affiliation: INSERM, U646, Université d'Angers, Angers, F-49100, France. skaya2@free.fr

ABSTRACT

Purpose: Radiotherapy has been shown to be an effective for the treatment human glioma and consists of 30 fractions of 2 Gy each for 6-7 weeks in the tumor volume with margins. However. in preclinical studies, many different radiation schedules are used. The main purpose of this work was to review the relevant literature and to propose an external whole-brain irradiation (WBI) protocol for a rat 9L glioma model.

Materials and methods: 9L cells were implanted in the striatum of twenty 344-Fisher rats to induce a brain tumor. On day 8, animals were randomized in two groups: an untreated group and an irradiated group with three fractions of 6 Gy at day 8, 11 and 14. Survival and toxicity were assessed.

Results: Irradiated rats had significantly a longer survival (p = 0.01). No deaths occurred due to the treatment. Toxicities of reduced weight and alopecia were increased during the radiation period but no serious morbidity or mortality was observed. Moreover, abnormalities disappeared the week following the end of the therapeutic schedule.

Conclusions: Delivering 18 Gy in 3 fractions of 6 Gy every 3 days, with mild anaesthesia, is safe, easy to reproduce and allows for standardisation in preclinical studies of different treatment regimens glioma rat model.

Show MeSH
Related in: MedlinePlus