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External irradiation models for intracranial 9L glioma studies.

Vinchon-Petit S, Jarnet D, Jadaud E, Feuvret L, Garcion E, Menei P - J. Exp. Clin. Cancer Res. (2010)

Bottom Line: Toxicities of reduced weight and alopecia were increased during the radiation period but no serious morbidity or mortality was observed.Moreover, abnormalities disappeared the week following the end of the therapeutic schedule.Delivering 18 Gy in 3 fractions of 6 Gy every 3 days, with mild anaesthesia, is safe, easy to reproduce and allows for standardisation in preclinical studies of different treatment regimens glioma rat model.

View Article: PubMed Central - HTML - PubMed

Affiliation: INSERM, U646, Université d'Angers, Angers, F-49100, France. skaya2@free.fr

ABSTRACT

Purpose: Radiotherapy has been shown to be an effective for the treatment human glioma and consists of 30 fractions of 2 Gy each for 6-7 weeks in the tumor volume with margins. However. in preclinical studies, many different radiation schedules are used. The main purpose of this work was to review the relevant literature and to propose an external whole-brain irradiation (WBI) protocol for a rat 9L glioma model.

Materials and methods: 9L cells were implanted in the striatum of twenty 344-Fisher rats to induce a brain tumor. On day 8, animals were randomized in two groups: an untreated group and an irradiated group with three fractions of 6 Gy at day 8, 11 and 14. Survival and toxicity were assessed.

Results: Irradiated rats had significantly a longer survival (p = 0.01). No deaths occurred due to the treatment. Toxicities of reduced weight and alopecia were increased during the radiation period but no serious morbidity or mortality was observed. Moreover, abnormalities disappeared the week following the end of the therapeutic schedule.

Conclusions: Delivering 18 Gy in 3 fractions of 6 Gy every 3 days, with mild anaesthesia, is safe, easy to reproduce and allows for standardisation in preclinical studies of different treatment regimens glioma rat model.

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Related in: MedlinePlus

Radiation therapy position. An equivalent tissue of 1.5 cm was laid on the rat head in order to improve the dose distribution in brain.
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Figure 1: Radiation therapy position. An equivalent tissue of 1.5 cm was laid on the rat head in order to improve the dose distribution in brain.

Mentions: Rats were irradiated using a 6-MV linear accelerator (Saturn 41 type, Varian Medical Systems, Salt Lake City, USA), under mild anaesthesia by isoflurane (4.5% during 2 minutes then 2% for the treatment) + O2 3 L/min. Oxygen masks were connected and four rats were placed in a reproducible way, in a prone position on the linac couch with laser alignment. The WBI was delivered by one photon beam (6 MV-energy, DSP 100 and 4 Gy/min). The radiation field was 15 × 15 cm at source-axis distance of 100 cm. The isocenter was in the midline of the brain and the posterior limit of the field corresponded to the line passing by the posterior part of the 2 ears (Figure 1).


External irradiation models for intracranial 9L glioma studies.

Vinchon-Petit S, Jarnet D, Jadaud E, Feuvret L, Garcion E, Menei P - J. Exp. Clin. Cancer Res. (2010)

Radiation therapy position. An equivalent tissue of 1.5 cm was laid on the rat head in order to improve the dose distribution in brain.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2992475&req=5

Figure 1: Radiation therapy position. An equivalent tissue of 1.5 cm was laid on the rat head in order to improve the dose distribution in brain.
Mentions: Rats were irradiated using a 6-MV linear accelerator (Saturn 41 type, Varian Medical Systems, Salt Lake City, USA), under mild anaesthesia by isoflurane (4.5% during 2 minutes then 2% for the treatment) + O2 3 L/min. Oxygen masks were connected and four rats were placed in a reproducible way, in a prone position on the linac couch with laser alignment. The WBI was delivered by one photon beam (6 MV-energy, DSP 100 and 4 Gy/min). The radiation field was 15 × 15 cm at source-axis distance of 100 cm. The isocenter was in the midline of the brain and the posterior limit of the field corresponded to the line passing by the posterior part of the 2 ears (Figure 1).

Bottom Line: Toxicities of reduced weight and alopecia were increased during the radiation period but no serious morbidity or mortality was observed.Moreover, abnormalities disappeared the week following the end of the therapeutic schedule.Delivering 18 Gy in 3 fractions of 6 Gy every 3 days, with mild anaesthesia, is safe, easy to reproduce and allows for standardisation in preclinical studies of different treatment regimens glioma rat model.

View Article: PubMed Central - HTML - PubMed

Affiliation: INSERM, U646, Université d'Angers, Angers, F-49100, France. skaya2@free.fr

ABSTRACT

Purpose: Radiotherapy has been shown to be an effective for the treatment human glioma and consists of 30 fractions of 2 Gy each for 6-7 weeks in the tumor volume with margins. However. in preclinical studies, many different radiation schedules are used. The main purpose of this work was to review the relevant literature and to propose an external whole-brain irradiation (WBI) protocol for a rat 9L glioma model.

Materials and methods: 9L cells were implanted in the striatum of twenty 344-Fisher rats to induce a brain tumor. On day 8, animals were randomized in two groups: an untreated group and an irradiated group with three fractions of 6 Gy at day 8, 11 and 14. Survival and toxicity were assessed.

Results: Irradiated rats had significantly a longer survival (p = 0.01). No deaths occurred due to the treatment. Toxicities of reduced weight and alopecia were increased during the radiation period but no serious morbidity or mortality was observed. Moreover, abnormalities disappeared the week following the end of the therapeutic schedule.

Conclusions: Delivering 18 Gy in 3 fractions of 6 Gy every 3 days, with mild anaesthesia, is safe, easy to reproduce and allows for standardisation in preclinical studies of different treatment regimens glioma rat model.

Show MeSH
Related in: MedlinePlus