Limits...
Free radical scavenging activities of Cnidium officinale Makino and Ligusticum chuanxiong Hort. methanolic extracts.

Ramalingam M, Yong-Ki P - Pharmacogn Mag (2010)

Bottom Line: In addition, the cell viability and nitric oxide release were assayed using Neuro-2a (N2a) cells.The methanolic extracts of C. officinale and L. chuanxiong showed scavenging activities of free radicals with an additional antioxidant capacity.Results of the present study suggests that the extracts of C. officinale and L. chuanxiong have comparatively similar free radical scavenging activities in vitro and may have important health effects.

View Article: PubMed Central - PubMed

Affiliation: Oriental Medicine Research Institute, Dongguk University, 707, Seokjangdong, Gyeongju, Gyeongbuk 780-714, Republic of Korea.

ABSTRACT

Background: Antioxidants from natural resources possess multifaceted and importance of the activities provides substantial scope in neurodegenerative diseases. The aim of this study was to assess and compare the free radical scavenging activities of Cnidium officinale and Ligusticum chuanxiong, which are closely related species.

Materials and methods: The scavenging activities of plant materials were evaluated using Trolox equivalent antioxidant capacity (TEAC), oxygen radical absorbance capacity (ORAC) and 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide radical (O(2)·(-)), hydrogen peroxide (H(2)O(2)), hydroxyl (OH·), nitric oxide radical (NO·) and metal chelation. In addition, the cell viability and nitric oxide release were assayed using Neuro-2a (N2a) cells.

Results: The methanolic extracts of C. officinale and L. chuanxiong showed scavenging activities of free radicals with an additional antioxidant capacity. Moreover, the efficacy on the cell viability and nitric oxide release in cell culture model has been established.

Conclusion: Results of the present study suggests that the extracts of C. officinale and L. chuanxiong have comparatively similar free radical scavenging activities in vitro and may have important health effects.

No MeSH data available.


Related in: MedlinePlus

Effect of C. officinale and L. chuanxiong methanolic extracts on cell viability in N2a cells. Values are means ± SD of three measurements. *P < 0.05 compared with untreated normal (ANOVA/Tukey)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2992148&req=5

Figure 0001: Effect of C. officinale and L. chuanxiong methanolic extracts on cell viability in N2a cells. Values are means ± SD of three measurements. *P < 0.05 compared with untreated normal (ANOVA/Tukey)

Mentions: To determine the effects of C. officinale and L. chuanxiong on cell viability, the N2a cells were exposed to C. officinale and L. chuanxiong (50–500 µg/ml) for an incubation time of 1 h. In Figure 1, the MTT test after 1 h of incubation with C. officinale does not indicate any significant viability difference in treated N2a cell cultures in comparison with control. By MTT test after 1 h with L. chuanxiong, a significant increase of viability was observed in L. chuanxiong 500 µg/ml treated N2a cells in comparison with control. As shown in Figure 2, NO determination was performed after 1 h of incubation in the presence of C. officinale and L. chuanxiong (50–500 µg/ml). Treatment with L. chuanxiong did not decrease the release of NO significantly when compared to control, but 500 µg/ml C. officinale decreased significantly the NO release. From this result, it can be concluded that the methanolic extracts of C. officinale and L. chuanxiong, at the doses used, have no toxicity effects.


Free radical scavenging activities of Cnidium officinale Makino and Ligusticum chuanxiong Hort. methanolic extracts.

Ramalingam M, Yong-Ki P - Pharmacogn Mag (2010)

Effect of C. officinale and L. chuanxiong methanolic extracts on cell viability in N2a cells. Values are means ± SD of three measurements. *P < 0.05 compared with untreated normal (ANOVA/Tukey)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2992148&req=5

Figure 0001: Effect of C. officinale and L. chuanxiong methanolic extracts on cell viability in N2a cells. Values are means ± SD of three measurements. *P < 0.05 compared with untreated normal (ANOVA/Tukey)
Mentions: To determine the effects of C. officinale and L. chuanxiong on cell viability, the N2a cells were exposed to C. officinale and L. chuanxiong (50–500 µg/ml) for an incubation time of 1 h. In Figure 1, the MTT test after 1 h of incubation with C. officinale does not indicate any significant viability difference in treated N2a cell cultures in comparison with control. By MTT test after 1 h with L. chuanxiong, a significant increase of viability was observed in L. chuanxiong 500 µg/ml treated N2a cells in comparison with control. As shown in Figure 2, NO determination was performed after 1 h of incubation in the presence of C. officinale and L. chuanxiong (50–500 µg/ml). Treatment with L. chuanxiong did not decrease the release of NO significantly when compared to control, but 500 µg/ml C. officinale decreased significantly the NO release. From this result, it can be concluded that the methanolic extracts of C. officinale and L. chuanxiong, at the doses used, have no toxicity effects.

Bottom Line: In addition, the cell viability and nitric oxide release were assayed using Neuro-2a (N2a) cells.The methanolic extracts of C. officinale and L. chuanxiong showed scavenging activities of free radicals with an additional antioxidant capacity.Results of the present study suggests that the extracts of C. officinale and L. chuanxiong have comparatively similar free radical scavenging activities in vitro and may have important health effects.

View Article: PubMed Central - PubMed

Affiliation: Oriental Medicine Research Institute, Dongguk University, 707, Seokjangdong, Gyeongju, Gyeongbuk 780-714, Republic of Korea.

ABSTRACT

Background: Antioxidants from natural resources possess multifaceted and importance of the activities provides substantial scope in neurodegenerative diseases. The aim of this study was to assess and compare the free radical scavenging activities of Cnidium officinale and Ligusticum chuanxiong, which are closely related species.

Materials and methods: The scavenging activities of plant materials were evaluated using Trolox equivalent antioxidant capacity (TEAC), oxygen radical absorbance capacity (ORAC) and 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide radical (O(2)·(-)), hydrogen peroxide (H(2)O(2)), hydroxyl (OH·), nitric oxide radical (NO·) and metal chelation. In addition, the cell viability and nitric oxide release were assayed using Neuro-2a (N2a) cells.

Results: The methanolic extracts of C. officinale and L. chuanxiong showed scavenging activities of free radicals with an additional antioxidant capacity. Moreover, the efficacy on the cell viability and nitric oxide release in cell culture model has been established.

Conclusion: Results of the present study suggests that the extracts of C. officinale and L. chuanxiong have comparatively similar free radical scavenging activities in vitro and may have important health effects.

No MeSH data available.


Related in: MedlinePlus