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Fractional extraction and structural characterization of opium poppy and cotton stalks hemicelluloses.

Cengiz M, Dincturk OD, Turgut Sahin H - Pharmacogn Mag (2010)

Bottom Line: The 2.0 and 3.0% H(2)O(2)extractions resulted in a yield of 0.8 and 0.71%, respectively, accounting for 3.2 and 2.9% of the hemicelluloses present in the opium poppy stalks.A similar result was also obtained for cotton stalks.It was found that alkaline peroxide is an effective agent for solubilization of hemicelluloses from opium poppy and cotton stalks.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Faculty of Art and Sciences, S.Demirel University, East Campus, 32260 Cunur-Isparta, Turkey.

ABSTRACT
Hemicellulosic moieties from opium poppy and cotton stalks were solublized in water at varying alkali concentrations (NaOH) and peroxide (H(2)O(2)). The hemicelluloses were then be precipitated from the solutions by acidification. The 2.0 and 3.0% H(2)O(2)extractions resulted in a yield of 0.8 and 0.71%, respectively, accounting for 3.2 and 2.9% of the hemicelluloses present in the opium poppy stalks. A similar result was also obtained for cotton stalks. It was found that alkaline peroxide is an effective agent for solubilization of hemicelluloses from opium poppy and cotton stalks.

No MeSH data available.


FT-IR spectra of hemicellulosic fractions released during the sequential treatment of opium poppy with water (A), 2.0 M NaOH (B), 0.5% (C) and 3.0% H2O2 (D)
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Figure 0001: FT-IR spectra of hemicellulosic fractions released during the sequential treatment of opium poppy with water (A), 2.0 M NaOH (B), 0.5% (C) and 3.0% H2O2 (D)

Mentions: Figure 1 illustrates the comparative FT-IR diagrams of hemicellulosic moieties on the fractional extracted oppium popy stalks. Those spectra clearly show the typical signal pattern for hemicellulosic moiety that are various forms of glucose, xylan and arabinose structures of the substrates which were chracterized earlier by Çopuroğlu (2004). However, the water solubilized hemicellulosic fractions shown typical stretching and bending vibrations of C–O, C–C, C–OH, and C–O–C groups at around 1000 cm-1 [Figure 1A]. The bands between 1175 and 1000 cm-1are typical of xylans. In the carbonyl stretching region, in addition to an intensive signal due to the absorbed water at 1640 cm-1, a small band at 1740 cm-1in alkali-soluble hemicellulosic fraction [Figure 1B] is assigned to the acetyl, uronic, and ferulic ester groups of the polysaccharides, whereas the absence of this signal in the spectrum of water-soluble hemicellulosic fraction demonstrated that the water treatment under the condition used cleaved this ester bond from the hemicelluloses. Figure 1 also illustrates the FT-IR spectra of hemicellulosic fractions released during the treatment with 0.5% [Figure 1C] and 3.0% H2O2 [Figure 1D]. The most obvious feature is the similarity of the spectra, indicating a similar structure of the hemicelluloses. As expected, the absence of a signal around 1720 cm-1for carbonyl stretching in all these two [Figure 1C and D] spectra indicated that the sequential treatments with alkaline peroxide under the conditions given did not significantly attack the glycosidic linkages and hydroxyl groups of hemicelluloses.


Fractional extraction and structural characterization of opium poppy and cotton stalks hemicelluloses.

Cengiz M, Dincturk OD, Turgut Sahin H - Pharmacogn Mag (2010)

FT-IR spectra of hemicellulosic fractions released during the sequential treatment of opium poppy with water (A), 2.0 M NaOH (B), 0.5% (C) and 3.0% H2O2 (D)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2992146&req=5

Figure 0001: FT-IR spectra of hemicellulosic fractions released during the sequential treatment of opium poppy with water (A), 2.0 M NaOH (B), 0.5% (C) and 3.0% H2O2 (D)
Mentions: Figure 1 illustrates the comparative FT-IR diagrams of hemicellulosic moieties on the fractional extracted oppium popy stalks. Those spectra clearly show the typical signal pattern for hemicellulosic moiety that are various forms of glucose, xylan and arabinose structures of the substrates which were chracterized earlier by Çopuroğlu (2004). However, the water solubilized hemicellulosic fractions shown typical stretching and bending vibrations of C–O, C–C, C–OH, and C–O–C groups at around 1000 cm-1 [Figure 1A]. The bands between 1175 and 1000 cm-1are typical of xylans. In the carbonyl stretching region, in addition to an intensive signal due to the absorbed water at 1640 cm-1, a small band at 1740 cm-1in alkali-soluble hemicellulosic fraction [Figure 1B] is assigned to the acetyl, uronic, and ferulic ester groups of the polysaccharides, whereas the absence of this signal in the spectrum of water-soluble hemicellulosic fraction demonstrated that the water treatment under the condition used cleaved this ester bond from the hemicelluloses. Figure 1 also illustrates the FT-IR spectra of hemicellulosic fractions released during the treatment with 0.5% [Figure 1C] and 3.0% H2O2 [Figure 1D]. The most obvious feature is the similarity of the spectra, indicating a similar structure of the hemicelluloses. As expected, the absence of a signal around 1720 cm-1for carbonyl stretching in all these two [Figure 1C and D] spectra indicated that the sequential treatments with alkaline peroxide under the conditions given did not significantly attack the glycosidic linkages and hydroxyl groups of hemicelluloses.

Bottom Line: The 2.0 and 3.0% H(2)O(2)extractions resulted in a yield of 0.8 and 0.71%, respectively, accounting for 3.2 and 2.9% of the hemicelluloses present in the opium poppy stalks.A similar result was also obtained for cotton stalks.It was found that alkaline peroxide is an effective agent for solubilization of hemicelluloses from opium poppy and cotton stalks.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Faculty of Art and Sciences, S.Demirel University, East Campus, 32260 Cunur-Isparta, Turkey.

ABSTRACT
Hemicellulosic moieties from opium poppy and cotton stalks were solublized in water at varying alkali concentrations (NaOH) and peroxide (H(2)O(2)). The hemicelluloses were then be precipitated from the solutions by acidification. The 2.0 and 3.0% H(2)O(2)extractions resulted in a yield of 0.8 and 0.71%, respectively, accounting for 3.2 and 2.9% of the hemicelluloses present in the opium poppy stalks. A similar result was also obtained for cotton stalks. It was found that alkaline peroxide is an effective agent for solubilization of hemicelluloses from opium poppy and cotton stalks.

No MeSH data available.