Limits...
Use of in vitro assays to assess the potential antiproliferative and cytotoxic effects of saffron (Crocus sativus L.) in human lung cancer cell line.

Samarghandian S, Boskabady MH, Davoodi S - Pharmacogn Mag (2010)

Bottom Line: However, the extract at different concentrations could not significantly decrease the cell viability in L929 cells.Morphology of MCF7 cells treated with the ethanolic extract confirmed the MTT results.We also showed that even higher concentrations of saffron is safe for L929, but the extract exerts pro-apoptotic effects in a lung cancer-derived cell line and could be considered as a potential chemotherapeutic agent in lung cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, School of Medicine, Mashhad University Medical Sciences, Mashhad, Iran.

ABSTRACT

Background: Saffron is harvested from the dried, dark red stigmas of Crocus sativus flowers. It is used as a spice for flavoring and coloring food as a perfume. It is often used for treating several diseases. We investigated the potential of the ethanolic extract of saffron to induce antiproliferative and cytotoxic effects in cultured carcinomic human alveolar basal epithelial cells in comparison with non-malignant (L929) cells.

Materials and methods: Both cells were cultured in Dulbecco's modified Eagle's medium and treated with the ethanolic extract of saffron at various concentrations for two consecutive days. Our study resulted in sequences of events marked by apoptosis, such as loss of cell viability, morphology changes that were evaluated by MTT assay and invert-microscope, respectively.

Results: The results showed that the ethanolic extract of saffron decreased cell viability in malignant cells as a concentration and time-dependent manner. The IC (50) values against the lung cancer cell line were determined as 1500 and 565 μg/ml after 24 and 48 h, respectively. However, the extract at different concentrations could not significantly decrease the cell viability in L929 cells. Morphology of MCF7 cells treated with the ethanolic extract confirmed the MTT results.

Conclusion: We also showed that even higher concentrations of saffron is safe for L929, but the extract exerts pro-apoptotic effects in a lung cancer-derived cell line and could be considered as a potential chemotherapeutic agent in lung cancer.

No MeSH data available.


Related in: MedlinePlus

Comparison cytotoxicty effect of saffron extract on cell viability of lung cancer cell (A549) and non-malignant cell (L929) line. Morphological changes of cells after treatment with different concentration of saffron extract for 48 hours. A=control; B=500 (μg/ml); C= 1500 (μg/ml) saffron extract
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2992145&req=5

Figure 0004: Comparison cytotoxicty effect of saffron extract on cell viability of lung cancer cell (A549) and non-malignant cell (L929) line. Morphological changes of cells after treatment with different concentration of saffron extract for 48 hours. A=control; B=500 (μg/ml); C= 1500 (μg/ml) saffron extract

Mentions: In order to evaluate the effect of the ethanolic extract of saffron on the growth of human lung cancer cells and L929, the cells were incubated with different concentrations of the ethanolic saffron extract (500, 1000, 1500 and 2000 μg/ml) for 24 and 48 h, and their growth inhibitory effects were compared. The impact of the saffron extract on cell viability was quantitated by the MTT assay. The ethanolic saffron extract showed significantly high growth inhibitory effects on the lung cancer cell line in a concentration and time-dependent manner compared with the L929 cell line. As shown in Figure 3, the ethanolic extract of saffron (1000, 1500, 2000 μg/ml) decreased the cell viability in malignant cells but not in non-malignant cells after 24 h. This toxicity was consistent with morphologic changes. However, the extract, at different concentrations, could not significantly decrease the cell viability in L929 cells. After 48 h, a lower concentration of the ethanolic extract of saffron (500 μg/ml) dramatically decreased cell viability in the lung cancer cell line so that significant growth inhibition was initiated at 500 μg/ml after 48 h [Figure 4]. Therefore, exposure of the lung cancer cell line for 24 h significantly decreased the number of cells at a dose of 1000 μg/ml (P < 0.01), 1500 and 2000 μg/ml (P < 0.001). The dose-inducing 50% cell growth inhibition (IC50) against malignant cells was determined at 1500 and 565 μg/ml after 24 and 48 h, respectively [Table 1].


Use of in vitro assays to assess the potential antiproliferative and cytotoxic effects of saffron (Crocus sativus L.) in human lung cancer cell line.

Samarghandian S, Boskabady MH, Davoodi S - Pharmacogn Mag (2010)

Comparison cytotoxicty effect of saffron extract on cell viability of lung cancer cell (A549) and non-malignant cell (L929) line. Morphological changes of cells after treatment with different concentration of saffron extract for 48 hours. A=control; B=500 (μg/ml); C= 1500 (μg/ml) saffron extract
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2992145&req=5

Figure 0004: Comparison cytotoxicty effect of saffron extract on cell viability of lung cancer cell (A549) and non-malignant cell (L929) line. Morphological changes of cells after treatment with different concentration of saffron extract for 48 hours. A=control; B=500 (μg/ml); C= 1500 (μg/ml) saffron extract
Mentions: In order to evaluate the effect of the ethanolic extract of saffron on the growth of human lung cancer cells and L929, the cells were incubated with different concentrations of the ethanolic saffron extract (500, 1000, 1500 and 2000 μg/ml) for 24 and 48 h, and their growth inhibitory effects were compared. The impact of the saffron extract on cell viability was quantitated by the MTT assay. The ethanolic saffron extract showed significantly high growth inhibitory effects on the lung cancer cell line in a concentration and time-dependent manner compared with the L929 cell line. As shown in Figure 3, the ethanolic extract of saffron (1000, 1500, 2000 μg/ml) decreased the cell viability in malignant cells but not in non-malignant cells after 24 h. This toxicity was consistent with morphologic changes. However, the extract, at different concentrations, could not significantly decrease the cell viability in L929 cells. After 48 h, a lower concentration of the ethanolic extract of saffron (500 μg/ml) dramatically decreased cell viability in the lung cancer cell line so that significant growth inhibition was initiated at 500 μg/ml after 48 h [Figure 4]. Therefore, exposure of the lung cancer cell line for 24 h significantly decreased the number of cells at a dose of 1000 μg/ml (P < 0.01), 1500 and 2000 μg/ml (P < 0.001). The dose-inducing 50% cell growth inhibition (IC50) against malignant cells was determined at 1500 and 565 μg/ml after 24 and 48 h, respectively [Table 1].

Bottom Line: However, the extract at different concentrations could not significantly decrease the cell viability in L929 cells.Morphology of MCF7 cells treated with the ethanolic extract confirmed the MTT results.We also showed that even higher concentrations of saffron is safe for L929, but the extract exerts pro-apoptotic effects in a lung cancer-derived cell line and could be considered as a potential chemotherapeutic agent in lung cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, School of Medicine, Mashhad University Medical Sciences, Mashhad, Iran.

ABSTRACT

Background: Saffron is harvested from the dried, dark red stigmas of Crocus sativus flowers. It is used as a spice for flavoring and coloring food as a perfume. It is often used for treating several diseases. We investigated the potential of the ethanolic extract of saffron to induce antiproliferative and cytotoxic effects in cultured carcinomic human alveolar basal epithelial cells in comparison with non-malignant (L929) cells.

Materials and methods: Both cells were cultured in Dulbecco's modified Eagle's medium and treated with the ethanolic extract of saffron at various concentrations for two consecutive days. Our study resulted in sequences of events marked by apoptosis, such as loss of cell viability, morphology changes that were evaluated by MTT assay and invert-microscope, respectively.

Results: The results showed that the ethanolic extract of saffron decreased cell viability in malignant cells as a concentration and time-dependent manner. The IC (50) values against the lung cancer cell line were determined as 1500 and 565 μg/ml after 24 and 48 h, respectively. However, the extract at different concentrations could not significantly decrease the cell viability in L929 cells. Morphology of MCF7 cells treated with the ethanolic extract confirmed the MTT results.

Conclusion: We also showed that even higher concentrations of saffron is safe for L929, but the extract exerts pro-apoptotic effects in a lung cancer-derived cell line and could be considered as a potential chemotherapeutic agent in lung cancer.

No MeSH data available.


Related in: MedlinePlus