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Cytotoxic and growth inhibitory effects of the methanol extract Struchium sparganophora Ktze (Asteraceae) leaves.

Ayinde BA, Agbakwuru U - Pharmacogn Mag (2010)

Bottom Line: At the same concentration, the chloroform and the aqueous fractions showed 32.51 and 43.81% inhibitions.The plant material was observed to contain alkaloids, tannins, saponins and flavonoids, with no traces of anthracene derivatives.The results suggest the probable use of the plant in preparing recipes for tumor-related ailments.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacognosy, Faculty of Pharmacy, University of Benin, Benin City, Nigeria.

ABSTRACT

Background: Global research into medicinal plants used in treating tumor-related ailments has become imperative due to the emergence of various forms of cancer diseases. Usually consumed as a vegetable, Struchium sparganophora is indicated in traditional herbal medicine as one of the plants used in treating tumor-related ailments.

Materials and methods: This claim was examined using bench-top assay methods involving the cytotoxicity of the methanol extract of the leaves to tadpoles of Raniceps ranninus at 10, 20, 40 and 80 μg/ml. Also, the growth inhibitory effects of the extract on guinea corn radicle at 0.5, 1.0, 2 and 4 mg/ml in addition to evaluation of the phytochemical constituents of the leaves was performed. After 24 h, the crude extract and the chloroform fraction produced the highest cytotoxicity of 96.67 ± 4.71%, each at a concentration of 80 μg/ml, while the aqueous fraction produced 100% cytotoxicity at a concentration of 20 μg/ml.

Results: The crude extract had an LC50 of 26 μg/ml, the chloroform fraction had 6.25 while the aqueous fraction had 5 μg/ml. On the inhibition of the guinea corn radicle growth, after 96 h, the controls had an average length of 67.81 ± 2.6 mm, whereas the seeds treated with 4 mg/ml of the crude extract had an average length of 35.83 ±1.75 mm, indicating 47.81% reduction in length. At the same concentration, the chloroform and the aqueous fractions showed 32.51 and 43.81% inhibitions. The plant material was observed to contain alkaloids, tannins, saponins and flavonoids, with no traces of anthracene derivatives.

Conclusion: The results suggest the probable use of the plant in preparing recipes for tumor-related ailments.

No MeSH data available.


Related in: MedlinePlus

Inhibitory effects of the chloroform fraction of S. sparganophora leaves on length of Sorghum bicolor radicle
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Figure 0004: Inhibitory effects of the chloroform fraction of S. sparganophora leaves on length of Sorghum bicolor radicle

Mentions: The various concentrations of the aqueous and chloroform fractions were equally observed to inhibit the growth of the radicles of the seeds. The aqueous fraction was observed to be more effective than the chloroform fraction. At 96 h, the average length of 51.4 ± 2.25 mm in the controls was reduced to 31.48 ± 1.89 mm and 28.88 ±2.09 mm in seeds treated with 2 and 4 mg/ml concentrations, showing 38.76 and 43.81%, reductions respectively [Figure 3]. The chloroform fraction reduced the control lengths of 44.45 ± 2.67 to 31.57 ± 2.55 and 30.0 ± 4.3 mm, indicating 29 and 32.51% reductions, respectively [Figure 4].


Cytotoxic and growth inhibitory effects of the methanol extract Struchium sparganophora Ktze (Asteraceae) leaves.

Ayinde BA, Agbakwuru U - Pharmacogn Mag (2010)

Inhibitory effects of the chloroform fraction of S. sparganophora leaves on length of Sorghum bicolor radicle
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2992142&req=5

Figure 0004: Inhibitory effects of the chloroform fraction of S. sparganophora leaves on length of Sorghum bicolor radicle
Mentions: The various concentrations of the aqueous and chloroform fractions were equally observed to inhibit the growth of the radicles of the seeds. The aqueous fraction was observed to be more effective than the chloroform fraction. At 96 h, the average length of 51.4 ± 2.25 mm in the controls was reduced to 31.48 ± 1.89 mm and 28.88 ±2.09 mm in seeds treated with 2 and 4 mg/ml concentrations, showing 38.76 and 43.81%, reductions respectively [Figure 3]. The chloroform fraction reduced the control lengths of 44.45 ± 2.67 to 31.57 ± 2.55 and 30.0 ± 4.3 mm, indicating 29 and 32.51% reductions, respectively [Figure 4].

Bottom Line: At the same concentration, the chloroform and the aqueous fractions showed 32.51 and 43.81% inhibitions.The plant material was observed to contain alkaloids, tannins, saponins and flavonoids, with no traces of anthracene derivatives.The results suggest the probable use of the plant in preparing recipes for tumor-related ailments.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacognosy, Faculty of Pharmacy, University of Benin, Benin City, Nigeria.

ABSTRACT

Background: Global research into medicinal plants used in treating tumor-related ailments has become imperative due to the emergence of various forms of cancer diseases. Usually consumed as a vegetable, Struchium sparganophora is indicated in traditional herbal medicine as one of the plants used in treating tumor-related ailments.

Materials and methods: This claim was examined using bench-top assay methods involving the cytotoxicity of the methanol extract of the leaves to tadpoles of Raniceps ranninus at 10, 20, 40 and 80 μg/ml. Also, the growth inhibitory effects of the extract on guinea corn radicle at 0.5, 1.0, 2 and 4 mg/ml in addition to evaluation of the phytochemical constituents of the leaves was performed. After 24 h, the crude extract and the chloroform fraction produced the highest cytotoxicity of 96.67 ± 4.71%, each at a concentration of 80 μg/ml, while the aqueous fraction produced 100% cytotoxicity at a concentration of 20 μg/ml.

Results: The crude extract had an LC50 of 26 μg/ml, the chloroform fraction had 6.25 while the aqueous fraction had 5 μg/ml. On the inhibition of the guinea corn radicle growth, after 96 h, the controls had an average length of 67.81 ± 2.6 mm, whereas the seeds treated with 4 mg/ml of the crude extract had an average length of 35.83 ±1.75 mm, indicating 47.81% reduction in length. At the same concentration, the chloroform and the aqueous fractions showed 32.51 and 43.81% inhibitions. The plant material was observed to contain alkaloids, tannins, saponins and flavonoids, with no traces of anthracene derivatives.

Conclusion: The results suggest the probable use of the plant in preparing recipes for tumor-related ailments.

No MeSH data available.


Related in: MedlinePlus