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Tissue culture and generation of autotetraploid plants of Sophora flavescens Aiton.

Kun-Hua W, Shan-Lin G, He-Ping H - Pharmacogn Mag (2010)

Bottom Line: The chromosome number of the autotetraploid plantlet was 2n = 4× = 36.All tetraploid plants showed typical polyploid characteristics.Obtained autotetraploid lines will be of important genetic and breeding value and can be used for further selection and plant breeding.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics and Breeding, China Pharmaceutical University, Nanjing, Jiangsu - 211 198, People's Republic of China.

ABSTRACT

Background: Sophora flavescens Aiton is an important medicinal plant in China. Early in vitro researches of S. flavescens were focused on callus induction and cell suspension culture, only a few were concerned with in vitro multiplication.

Objective: To establish and optimize the rapid propagation technology of S. flavescens and to generate and characterize polyploid plants of S. flavescens.

Materials and methods: The different concentrations of 6-benzylaminopurine (BAP), indole-3-acetic acid (IAA) and kinetin (KT) were used to establish and screen the optimal rapid propagation technology of S. flavescens by orthogonal test; 0.2% colchicine solution was used to induce polyploid plants and the induced buds were identified by root-tip chromosome determination and stomatal apparatus observation.

Results: A large number of buds could be induced directly from epicotyl and hypocotyl explants on the Murashige and Skoog medium (MS; 1962) supplemented with 1.4-1.6 mg/l 6-benzylaminopurine (BAP) and 0.3 mg/l indole-3-acetic acid (IAA). More than 50 lines of autotetraploid plants were obtained. The chromosome number of the autotetraploid plantlet was 2n = 4× = 36. All tetraploid plants showed typical polyploid characteristics.

Conclusion: Obtained autotetraploid lines will be of important genetic and breeding value and can be used for further selection and plant breeding.

No MeSH data available.


Related in: MedlinePlus

(a) The chromosome of diploid plant, 2n=2×=18 (bar: 6.04 × 10-4 cm). (b) The chromosome of tetraploid plant, 2n=4×=36 (bar: 6.04 × 10-4 cm). (c,d). Stomatal apparatus of diploid and tetraploid plants in Sophora flavescens from glasshouse. Each Stomatal apparatus was obtained from the same part of diploid (c) and tetraploid (d) unifoliate leaves in the glasshouse (bar: 7.36 × 10-3 cm)
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Figure 0002: (a) The chromosome of diploid plant, 2n=2×=18 (bar: 6.04 × 10-4 cm). (b) The chromosome of tetraploid plant, 2n=4×=36 (bar: 6.04 × 10-4 cm). (c,d). Stomatal apparatus of diploid and tetraploid plants in Sophora flavescens from glasshouse. Each Stomatal apparatus was obtained from the same part of diploid (c) and tetraploid (d) unifoliate leaves in the glasshouse (bar: 7.36 × 10-3 cm)

Mentions: Chromosome determination was performed on root tips of the rooted plantlets. According to the chromosome counts, 51 plantlets were tetraploid. The data in Table 6 indicated that immersing buds in the 0.2% (w/v) colchicine solution for 12, 24, 36, 48, 60, 72, 84, and 96 h was efficient for the induction of buds to produce polyploid buds [Table 6]. The percentage of buds with polyploid buds was 28% when immersing buds in 0.2% colchicine for 48 h. This is by far the highest induction ratio in our experiments. Chromosome counts revealed that the tetraploid plantlets had 36 chromosomes (4× = 36) [Figures 2a and b].


Tissue culture and generation of autotetraploid plants of Sophora flavescens Aiton.

Kun-Hua W, Shan-Lin G, He-Ping H - Pharmacogn Mag (2010)

(a) The chromosome of diploid plant, 2n=2×=18 (bar: 6.04 × 10-4 cm). (b) The chromosome of tetraploid plant, 2n=4×=36 (bar: 6.04 × 10-4 cm). (c,d). Stomatal apparatus of diploid and tetraploid plants in Sophora flavescens from glasshouse. Each Stomatal apparatus was obtained from the same part of diploid (c) and tetraploid (d) unifoliate leaves in the glasshouse (bar: 7.36 × 10-3 cm)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2992141&req=5

Figure 0002: (a) The chromosome of diploid plant, 2n=2×=18 (bar: 6.04 × 10-4 cm). (b) The chromosome of tetraploid plant, 2n=4×=36 (bar: 6.04 × 10-4 cm). (c,d). Stomatal apparatus of diploid and tetraploid plants in Sophora flavescens from glasshouse. Each Stomatal apparatus was obtained from the same part of diploid (c) and tetraploid (d) unifoliate leaves in the glasshouse (bar: 7.36 × 10-3 cm)
Mentions: Chromosome determination was performed on root tips of the rooted plantlets. According to the chromosome counts, 51 plantlets were tetraploid. The data in Table 6 indicated that immersing buds in the 0.2% (w/v) colchicine solution for 12, 24, 36, 48, 60, 72, 84, and 96 h was efficient for the induction of buds to produce polyploid buds [Table 6]. The percentage of buds with polyploid buds was 28% when immersing buds in 0.2% colchicine for 48 h. This is by far the highest induction ratio in our experiments. Chromosome counts revealed that the tetraploid plantlets had 36 chromosomes (4× = 36) [Figures 2a and b].

Bottom Line: The chromosome number of the autotetraploid plantlet was 2n = 4× = 36.All tetraploid plants showed typical polyploid characteristics.Obtained autotetraploid lines will be of important genetic and breeding value and can be used for further selection and plant breeding.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics and Breeding, China Pharmaceutical University, Nanjing, Jiangsu - 211 198, People's Republic of China.

ABSTRACT

Background: Sophora flavescens Aiton is an important medicinal plant in China. Early in vitro researches of S. flavescens were focused on callus induction and cell suspension culture, only a few were concerned with in vitro multiplication.

Objective: To establish and optimize the rapid propagation technology of S. flavescens and to generate and characterize polyploid plants of S. flavescens.

Materials and methods: The different concentrations of 6-benzylaminopurine (BAP), indole-3-acetic acid (IAA) and kinetin (KT) were used to establish and screen the optimal rapid propagation technology of S. flavescens by orthogonal test; 0.2% colchicine solution was used to induce polyploid plants and the induced buds were identified by root-tip chromosome determination and stomatal apparatus observation.

Results: A large number of buds could be induced directly from epicotyl and hypocotyl explants on the Murashige and Skoog medium (MS; 1962) supplemented with 1.4-1.6 mg/l 6-benzylaminopurine (BAP) and 0.3 mg/l indole-3-acetic acid (IAA). More than 50 lines of autotetraploid plants were obtained. The chromosome number of the autotetraploid plantlet was 2n = 4× = 36. All tetraploid plants showed typical polyploid characteristics.

Conclusion: Obtained autotetraploid lines will be of important genetic and breeding value and can be used for further selection and plant breeding.

No MeSH data available.


Related in: MedlinePlus