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Tissue culture and generation of autotetraploid plants of Sophora flavescens Aiton.

Kun-Hua W, Shan-Lin G, He-Ping H - Pharmacogn Mag (2010)

Bottom Line: The chromosome number of the autotetraploid plantlet was 2n = 4× = 36.All tetraploid plants showed typical polyploid characteristics.Obtained autotetraploid lines will be of important genetic and breeding value and can be used for further selection and plant breeding.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics and Breeding, China Pharmaceutical University, Nanjing, Jiangsu - 211 198, People's Republic of China.

ABSTRACT

Background: Sophora flavescens Aiton is an important medicinal plant in China. Early in vitro researches of S. flavescens were focused on callus induction and cell suspension culture, only a few were concerned with in vitro multiplication.

Objective: To establish and optimize the rapid propagation technology of S. flavescens and to generate and characterize polyploid plants of S. flavescens.

Materials and methods: The different concentrations of 6-benzylaminopurine (BAP), indole-3-acetic acid (IAA) and kinetin (KT) were used to establish and screen the optimal rapid propagation technology of S. flavescens by orthogonal test; 0.2% colchicine solution was used to induce polyploid plants and the induced buds were identified by root-tip chromosome determination and stomatal apparatus observation.

Results: A large number of buds could be induced directly from epicotyl and hypocotyl explants on the Murashige and Skoog medium (MS; 1962) supplemented with 1.4-1.6 mg/l 6-benzylaminopurine (BAP) and 0.3 mg/l indole-3-acetic acid (IAA). More than 50 lines of autotetraploid plants were obtained. The chromosome number of the autotetraploid plantlet was 2n = 4× = 36. All tetraploid plants showed typical polyploid characteristics.

Conclusion: Obtained autotetraploid lines will be of important genetic and breeding value and can be used for further selection and plant breeding.

No MeSH data available.


Related in: MedlinePlus

The buds of Sophora flavescens Ait. (Leguminosae) on MS mediuma supplemented with 1.5 mg/l BAP and 0.3 mg/l IAA (bar: 0.154 cm)
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Figure 0001: The buds of Sophora flavescens Ait. (Leguminosae) on MS mediuma supplemented with 1.5 mg/l BAP and 0.3 mg/l IAA (bar: 0.154 cm)

Mentions: Based upon the orthogonal test, further optimization experiments using BAP concentrations of 1.0, 1.2, 1.4, 1.6, and 1.8 mg/l were combined with a fixed IAA concentration of 0.3 mg/l. The fastest bud growth rate was 5.90 ± 0.14 g/g for cultures on a medium supplemented with BAP at 1.6 mg/l and the highest bud multiplication time was 9.88 ± 0.09 for cultures on a medium supplemented with BAP at 1.4 mg/l [Table 5; Figure 1].Figure 1


Tissue culture and generation of autotetraploid plants of Sophora flavescens Aiton.

Kun-Hua W, Shan-Lin G, He-Ping H - Pharmacogn Mag (2010)

The buds of Sophora flavescens Ait. (Leguminosae) on MS mediuma supplemented with 1.5 mg/l BAP and 0.3 mg/l IAA (bar: 0.154 cm)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2992141&req=5

Figure 0001: The buds of Sophora flavescens Ait. (Leguminosae) on MS mediuma supplemented with 1.5 mg/l BAP and 0.3 mg/l IAA (bar: 0.154 cm)
Mentions: Based upon the orthogonal test, further optimization experiments using BAP concentrations of 1.0, 1.2, 1.4, 1.6, and 1.8 mg/l were combined with a fixed IAA concentration of 0.3 mg/l. The fastest bud growth rate was 5.90 ± 0.14 g/g for cultures on a medium supplemented with BAP at 1.6 mg/l and the highest bud multiplication time was 9.88 ± 0.09 for cultures on a medium supplemented with BAP at 1.4 mg/l [Table 5; Figure 1].Figure 1

Bottom Line: The chromosome number of the autotetraploid plantlet was 2n = 4× = 36.All tetraploid plants showed typical polyploid characteristics.Obtained autotetraploid lines will be of important genetic and breeding value and can be used for further selection and plant breeding.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics and Breeding, China Pharmaceutical University, Nanjing, Jiangsu - 211 198, People's Republic of China.

ABSTRACT

Background: Sophora flavescens Aiton is an important medicinal plant in China. Early in vitro researches of S. flavescens were focused on callus induction and cell suspension culture, only a few were concerned with in vitro multiplication.

Objective: To establish and optimize the rapid propagation technology of S. flavescens and to generate and characterize polyploid plants of S. flavescens.

Materials and methods: The different concentrations of 6-benzylaminopurine (BAP), indole-3-acetic acid (IAA) and kinetin (KT) were used to establish and screen the optimal rapid propagation technology of S. flavescens by orthogonal test; 0.2% colchicine solution was used to induce polyploid plants and the induced buds were identified by root-tip chromosome determination and stomatal apparatus observation.

Results: A large number of buds could be induced directly from epicotyl and hypocotyl explants on the Murashige and Skoog medium (MS; 1962) supplemented with 1.4-1.6 mg/l 6-benzylaminopurine (BAP) and 0.3 mg/l indole-3-acetic acid (IAA). More than 50 lines of autotetraploid plants were obtained. The chromosome number of the autotetraploid plantlet was 2n = 4× = 36. All tetraploid plants showed typical polyploid characteristics.

Conclusion: Obtained autotetraploid lines will be of important genetic and breeding value and can be used for further selection and plant breeding.

No MeSH data available.


Related in: MedlinePlus