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Determination of total flavonoids in three Sedum crude drugs by UV-Vis spectrophotometry.

Chen Y, Wang J, Wan D - Pharmacogn Mag (2010)

Bottom Line: A simple, rapid UV-Vis spectrophotometry method for the determination of total flavonoids in Sedum sarmentosum Bunge., S. lineare Thunb., and S. erythrostictum Migo. was developed, with a good linearity, precision, and stability.The detection wavelength was set at 500 nm, and an extraction solvent was optimized.Through the comparative study of multiple samples of the three plant drugs, their collected seasons and the habitats can be preliminarily ascertained, which may help to control the quality of the medicines to some extent.

View Article: PubMed Central - PubMed

Affiliation: Pharmacy College of South-Central University for Nationalities, Wuhan, Hubei Province - 430074, People' Republic China.

ABSTRACT
A simple, rapid UV-Vis spectrophotometry method for the determination of total flavonoids in Sedum sarmentosum Bunge., S. lineare Thunb., and S. erythrostictum Migo. was developed, with a good linearity, precision, and stability. The detection wavelength was set at 500 nm, and an extraction solvent was optimized. Through the comparative study of multiple samples of the three plant drugs, their collected seasons and the habitats can be preliminarily ascertained, which may help to control the quality of the medicines to some extent.

No MeSH data available.


The relationship between the analysis time and absorption
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Figure 0002: The relationship between the analysis time and absorption

Mentions: After the extraction of S. sarmentosum Bunge., the sample mentioned above was dealt with according to the procedures for the determination of total flavonoids; absorptions were measured every 20 min ranging from 0 min to 120 min [Figure 2]. It’s clear that the absorption of the solution was relatively stable (RSD = 0.78%) if the measurement was carried out within 120 min. Thus, all the analyses should be performed within 2 h after the color reaction.


Determination of total flavonoids in three Sedum crude drugs by UV-Vis spectrophotometry.

Chen Y, Wang J, Wan D - Pharmacogn Mag (2010)

The relationship between the analysis time and absorption
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2992136&req=5

Figure 0002: The relationship between the analysis time and absorption
Mentions: After the extraction of S. sarmentosum Bunge., the sample mentioned above was dealt with according to the procedures for the determination of total flavonoids; absorptions were measured every 20 min ranging from 0 min to 120 min [Figure 2]. It’s clear that the absorption of the solution was relatively stable (RSD = 0.78%) if the measurement was carried out within 120 min. Thus, all the analyses should be performed within 2 h after the color reaction.

Bottom Line: A simple, rapid UV-Vis spectrophotometry method for the determination of total flavonoids in Sedum sarmentosum Bunge., S. lineare Thunb., and S. erythrostictum Migo. was developed, with a good linearity, precision, and stability.The detection wavelength was set at 500 nm, and an extraction solvent was optimized.Through the comparative study of multiple samples of the three plant drugs, their collected seasons and the habitats can be preliminarily ascertained, which may help to control the quality of the medicines to some extent.

View Article: PubMed Central - PubMed

Affiliation: Pharmacy College of South-Central University for Nationalities, Wuhan, Hubei Province - 430074, People' Republic China.

ABSTRACT
A simple, rapid UV-Vis spectrophotometry method for the determination of total flavonoids in Sedum sarmentosum Bunge., S. lineare Thunb., and S. erythrostictum Migo. was developed, with a good linearity, precision, and stability. The detection wavelength was set at 500 nm, and an extraction solvent was optimized. Through the comparative study of multiple samples of the three plant drugs, their collected seasons and the habitats can be preliminarily ascertained, which may help to control the quality of the medicines to some extent.

No MeSH data available.