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Determination of total flavonoids in three Sedum crude drugs by UV-Vis spectrophotometry.

Chen Y, Wang J, Wan D - Pharmacogn Mag (2010)

Bottom Line: A simple, rapid UV-Vis spectrophotometry method for the determination of total flavonoids in Sedum sarmentosum Bunge., S. lineare Thunb., and S. erythrostictum Migo. was developed, with a good linearity, precision, and stability.The detection wavelength was set at 500 nm, and an extraction solvent was optimized.Through the comparative study of multiple samples of the three plant drugs, their collected seasons and the habitats can be preliminarily ascertained, which may help to control the quality of the medicines to some extent.

View Article: PubMed Central - PubMed

Affiliation: Pharmacy College of South-Central University for Nationalities, Wuhan, Hubei Province - 430074, People' Republic China.

ABSTRACT
A simple, rapid UV-Vis spectrophotometry method for the determination of total flavonoids in Sedum sarmentosum Bunge., S. lineare Thunb., and S. erythrostictum Migo. was developed, with a good linearity, precision, and stability. The detection wavelength was set at 500 nm, and an extraction solvent was optimized. Through the comparative study of multiple samples of the three plant drugs, their collected seasons and the habitats can be preliminarily ascertained, which may help to control the quality of the medicines to some extent.

No MeSH data available.


The relationship between the ethanol concentration and the flavonoid extraction yield of S. sarmentosum Bunge.
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Figure 0001: The relationship between the ethanol concentration and the flavonoid extraction yield of S. sarmentosum Bunge.

Mentions: Since flavonoids contain some hydroxyl groups, they often dissolve easily in alcohol or alcohol–water mixtures. The S. sarmentosum Bunge. sample (Jianshi, June 28, 2006) was employed here, trying to find out the optimum solvent for sample extraction. The relationship between alcohol concentration and the yield of the total flavonoids is shown in Figure 1. In the figure, we could see that the extraction yield kept increasing and reached the top when the ethanol concentration went up to 70%, and then began to fall sharply as the ethanol concentration continued growing. Hence, the optimum extraction solvent turned out to be 70% ethanol.


Determination of total flavonoids in three Sedum crude drugs by UV-Vis spectrophotometry.

Chen Y, Wang J, Wan D - Pharmacogn Mag (2010)

The relationship between the ethanol concentration and the flavonoid extraction yield of S. sarmentosum Bunge.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2992136&req=5

Figure 0001: The relationship between the ethanol concentration and the flavonoid extraction yield of S. sarmentosum Bunge.
Mentions: Since flavonoids contain some hydroxyl groups, they often dissolve easily in alcohol or alcohol–water mixtures. The S. sarmentosum Bunge. sample (Jianshi, June 28, 2006) was employed here, trying to find out the optimum solvent for sample extraction. The relationship between alcohol concentration and the yield of the total flavonoids is shown in Figure 1. In the figure, we could see that the extraction yield kept increasing and reached the top when the ethanol concentration went up to 70%, and then began to fall sharply as the ethanol concentration continued growing. Hence, the optimum extraction solvent turned out to be 70% ethanol.

Bottom Line: A simple, rapid UV-Vis spectrophotometry method for the determination of total flavonoids in Sedum sarmentosum Bunge., S. lineare Thunb., and S. erythrostictum Migo. was developed, with a good linearity, precision, and stability.The detection wavelength was set at 500 nm, and an extraction solvent was optimized.Through the comparative study of multiple samples of the three plant drugs, their collected seasons and the habitats can be preliminarily ascertained, which may help to control the quality of the medicines to some extent.

View Article: PubMed Central - PubMed

Affiliation: Pharmacy College of South-Central University for Nationalities, Wuhan, Hubei Province - 430074, People' Republic China.

ABSTRACT
A simple, rapid UV-Vis spectrophotometry method for the determination of total flavonoids in Sedum sarmentosum Bunge., S. lineare Thunb., and S. erythrostictum Migo. was developed, with a good linearity, precision, and stability. The detection wavelength was set at 500 nm, and an extraction solvent was optimized. Through the comparative study of multiple samples of the three plant drugs, their collected seasons and the habitats can be preliminarily ascertained, which may help to control the quality of the medicines to some extent.

No MeSH data available.