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Effect of korean red ginseng on testicular tissue injury after torsion and detorsion.

Kim YH, Kim GH, Shin JH, Kim KS, Lim JS - Korean J Urol (2010)

Bottom Line: Testicular weight was significantly higher in the T+K group than in the T group; however, there were no significant differences in hormone levels between the 4 groups.The mean level of ROS and superoxide production was significantly higher in the T group than in the C group, whereas administration of KRG attenuated this increase.Upon histologic evaluation, the T group was found to have cellular disarray, a lack of cellular cohesiveness, degenerative changes in the germinal cells, and less distinct changes in the seminiferous tubules, whereas the T+K group had a germinal epithelial layer that appeared nearly normal.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Chungnam National University School of Medicine, Daejeon, Korea.

ABSTRACT

Purpose: Korean red ginseng (KRG) is a potent antioxidant and a free radical scavenger. This study was designed to determine whether KRG could protect against dysfunction and oxidative stress induced by torsion-detorsion injury in rat testis.

Materials and methods: Six-week-old male Sprague-Dawley rats were randomly divided into four groups: a sham-operated control group (C), a sham-operated and KRG-treated group (K), a 2 hours torsion and detorsion group (T), and a 2 hours torsion and detorsion and KRG-treated group (T+K). We measured testis weight and hormone levels and reactive oxygen species (ROS) from the left renal vein. Superoxide generation was measured on the basis of lucigenin-enhanced chemiluminescence in testis tissue.

Results: Testicular weight was significantly higher in the T+K group than in the T group; however, there were no significant differences in hormone levels between the 4 groups. The mean level of ROS and superoxide production was significantly higher in the T group than in the C group, whereas administration of KRG attenuated this increase. Upon histologic evaluation, the T group was found to have cellular disarray, a lack of cellular cohesiveness, degenerative changes in the germinal cells, and less distinct changes in the seminiferous tubules, whereas the T+K group had a germinal epithelial layer that appeared nearly normal.

Conclusions: The present study demonstrated that KRG recovered the testis dysfunction in the rat testis by suppressing superoxide production.

No MeSH data available.


Related in: MedlinePlus

Lucigenin-enhanced chemiluminescence, reflective of testis superoxide levels. Luminescence was recorded in the absence or presence of NADPH (100 µmol/l). C: a sham-operated group, K: a sham-operated and KRG-treated group, T: a 2 hours torsion and detorsion group, T+K: a 2 hours torsion and detorsion and KRG-treated group.
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Figure 3: Lucigenin-enhanced chemiluminescence, reflective of testis superoxide levels. Luminescence was recorded in the absence or presence of NADPH (100 µmol/l). C: a sham-operated group, K: a sham-operated and KRG-treated group, T: a 2 hours torsion and detorsion group, T+K: a 2 hours torsion and detorsion and KRG-treated group.

Mentions: The basal testis superoxide was quantified by lucigenin-enhanced chemiluminescence. The superoxide levels were significantly higher in the T group (278.3±39.6 for the T group vs. 181±16.8 for the C group and 189.5±13.2 for the K group; all values RLU/1 mg tissue, p<0.05). The superoxide level decreased significantly in response to treatment with KRG (173.3±13.0 RLU/1 mg tissue, p<0.05). NADPH-driven superoxide production was markedly increased in the T group (Fig. 3), whereas it was significantly decreased by treatment with KRG.


Effect of korean red ginseng on testicular tissue injury after torsion and detorsion.

Kim YH, Kim GH, Shin JH, Kim KS, Lim JS - Korean J Urol (2010)

Lucigenin-enhanced chemiluminescence, reflective of testis superoxide levels. Luminescence was recorded in the absence or presence of NADPH (100 µmol/l). C: a sham-operated group, K: a sham-operated and KRG-treated group, T: a 2 hours torsion and detorsion group, T+K: a 2 hours torsion and detorsion and KRG-treated group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2991579&req=5

Figure 3: Lucigenin-enhanced chemiluminescence, reflective of testis superoxide levels. Luminescence was recorded in the absence or presence of NADPH (100 µmol/l). C: a sham-operated group, K: a sham-operated and KRG-treated group, T: a 2 hours torsion and detorsion group, T+K: a 2 hours torsion and detorsion and KRG-treated group.
Mentions: The basal testis superoxide was quantified by lucigenin-enhanced chemiluminescence. The superoxide levels were significantly higher in the T group (278.3±39.6 for the T group vs. 181±16.8 for the C group and 189.5±13.2 for the K group; all values RLU/1 mg tissue, p<0.05). The superoxide level decreased significantly in response to treatment with KRG (173.3±13.0 RLU/1 mg tissue, p<0.05). NADPH-driven superoxide production was markedly increased in the T group (Fig. 3), whereas it was significantly decreased by treatment with KRG.

Bottom Line: Testicular weight was significantly higher in the T+K group than in the T group; however, there were no significant differences in hormone levels between the 4 groups.The mean level of ROS and superoxide production was significantly higher in the T group than in the C group, whereas administration of KRG attenuated this increase.Upon histologic evaluation, the T group was found to have cellular disarray, a lack of cellular cohesiveness, degenerative changes in the germinal cells, and less distinct changes in the seminiferous tubules, whereas the T+K group had a germinal epithelial layer that appeared nearly normal.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Chungnam National University School of Medicine, Daejeon, Korea.

ABSTRACT

Purpose: Korean red ginseng (KRG) is a potent antioxidant and a free radical scavenger. This study was designed to determine whether KRG could protect against dysfunction and oxidative stress induced by torsion-detorsion injury in rat testis.

Materials and methods: Six-week-old male Sprague-Dawley rats were randomly divided into four groups: a sham-operated control group (C), a sham-operated and KRG-treated group (K), a 2 hours torsion and detorsion group (T), and a 2 hours torsion and detorsion and KRG-treated group (T+K). We measured testis weight and hormone levels and reactive oxygen species (ROS) from the left renal vein. Superoxide generation was measured on the basis of lucigenin-enhanced chemiluminescence in testis tissue.

Results: Testicular weight was significantly higher in the T+K group than in the T group; however, there were no significant differences in hormone levels between the 4 groups. The mean level of ROS and superoxide production was significantly higher in the T group than in the C group, whereas administration of KRG attenuated this increase. Upon histologic evaluation, the T group was found to have cellular disarray, a lack of cellular cohesiveness, degenerative changes in the germinal cells, and less distinct changes in the seminiferous tubules, whereas the T+K group had a germinal epithelial layer that appeared nearly normal.

Conclusions: The present study demonstrated that KRG recovered the testis dysfunction in the rat testis by suppressing superoxide production.

No MeSH data available.


Related in: MedlinePlus