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Effect of korean red ginseng on testicular tissue injury after torsion and detorsion.

Kim YH, Kim GH, Shin JH, Kim KS, Lim JS - Korean J Urol (2010)

Bottom Line: Testicular weight was significantly higher in the T+K group than in the T group; however, there were no significant differences in hormone levels between the 4 groups.The mean level of ROS and superoxide production was significantly higher in the T group than in the C group, whereas administration of KRG attenuated this increase.Upon histologic evaluation, the T group was found to have cellular disarray, a lack of cellular cohesiveness, degenerative changes in the germinal cells, and less distinct changes in the seminiferous tubules, whereas the T+K group had a germinal epithelial layer that appeared nearly normal.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Chungnam National University School of Medicine, Daejeon, Korea.

ABSTRACT

Purpose: Korean red ginseng (KRG) is a potent antioxidant and a free radical scavenger. This study was designed to determine whether KRG could protect against dysfunction and oxidative stress induced by torsion-detorsion injury in rat testis.

Materials and methods: Six-week-old male Sprague-Dawley rats were randomly divided into four groups: a sham-operated control group (C), a sham-operated and KRG-treated group (K), a 2 hours torsion and detorsion group (T), and a 2 hours torsion and detorsion and KRG-treated group (T+K). We measured testis weight and hormone levels and reactive oxygen species (ROS) from the left renal vein. Superoxide generation was measured on the basis of lucigenin-enhanced chemiluminescence in testis tissue.

Results: Testicular weight was significantly higher in the T+K group than in the T group; however, there were no significant differences in hormone levels between the 4 groups. The mean level of ROS and superoxide production was significantly higher in the T group than in the C group, whereas administration of KRG attenuated this increase. Upon histologic evaluation, the T group was found to have cellular disarray, a lack of cellular cohesiveness, degenerative changes in the germinal cells, and less distinct changes in the seminiferous tubules, whereas the T+K group had a germinal epithelial layer that appeared nearly normal.

Conclusions: The present study demonstrated that KRG recovered the testis dysfunction in the rat testis by suppressing superoxide production.

No MeSH data available.


Related in: MedlinePlus

Representative microphotographs of cross-sections of the seminiferous tubules from SD rats. (A: group C, B: group K, C: group T, D: group T+K) (H&E, ×400). C: a sham-operated group, K: a sham-operated and KRG-treated group, T: a 2 hours torsion and detorsion group, T+K: a 2 hours torsion and detorsion and KRG-treated group.
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Figure 2: Representative microphotographs of cross-sections of the seminiferous tubules from SD rats. (A: group C, B: group K, C: group T, D: group T+K) (H&E, ×400). C: a sham-operated group, K: a sham-operated and KRG-treated group, T: a 2 hours torsion and detorsion group, T+K: a 2 hours torsion and detorsion and KRG-treated group.

Mentions: The normal appearance of the testicular structure of rats was determined by evaluation of group C (Fig. 2A), which had a normal arrangement of germinative cells in the seminiferous tubules. The appearance of group K (Fig. 2B) was similar to that of group C. However, group T (Fig. 2C) showed cellular disarray, a lack of cellular cohesiveness, degenerative changes in the germinal cells, and less distinct changes in the seminiferous tubule. In the T+K group (Fig. 2D), the germinal epithelial layer appeared nearly normal.


Effect of korean red ginseng on testicular tissue injury after torsion and detorsion.

Kim YH, Kim GH, Shin JH, Kim KS, Lim JS - Korean J Urol (2010)

Representative microphotographs of cross-sections of the seminiferous tubules from SD rats. (A: group C, B: group K, C: group T, D: group T+K) (H&E, ×400). C: a sham-operated group, K: a sham-operated and KRG-treated group, T: a 2 hours torsion and detorsion group, T+K: a 2 hours torsion and detorsion and KRG-treated group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2991579&req=5

Figure 2: Representative microphotographs of cross-sections of the seminiferous tubules from SD rats. (A: group C, B: group K, C: group T, D: group T+K) (H&E, ×400). C: a sham-operated group, K: a sham-operated and KRG-treated group, T: a 2 hours torsion and detorsion group, T+K: a 2 hours torsion and detorsion and KRG-treated group.
Mentions: The normal appearance of the testicular structure of rats was determined by evaluation of group C (Fig. 2A), which had a normal arrangement of germinative cells in the seminiferous tubules. The appearance of group K (Fig. 2B) was similar to that of group C. However, group T (Fig. 2C) showed cellular disarray, a lack of cellular cohesiveness, degenerative changes in the germinal cells, and less distinct changes in the seminiferous tubule. In the T+K group (Fig. 2D), the germinal epithelial layer appeared nearly normal.

Bottom Line: Testicular weight was significantly higher in the T+K group than in the T group; however, there were no significant differences in hormone levels between the 4 groups.The mean level of ROS and superoxide production was significantly higher in the T group than in the C group, whereas administration of KRG attenuated this increase.Upon histologic evaluation, the T group was found to have cellular disarray, a lack of cellular cohesiveness, degenerative changes in the germinal cells, and less distinct changes in the seminiferous tubules, whereas the T+K group had a germinal epithelial layer that appeared nearly normal.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Chungnam National University School of Medicine, Daejeon, Korea.

ABSTRACT

Purpose: Korean red ginseng (KRG) is a potent antioxidant and a free radical scavenger. This study was designed to determine whether KRG could protect against dysfunction and oxidative stress induced by torsion-detorsion injury in rat testis.

Materials and methods: Six-week-old male Sprague-Dawley rats were randomly divided into four groups: a sham-operated control group (C), a sham-operated and KRG-treated group (K), a 2 hours torsion and detorsion group (T), and a 2 hours torsion and detorsion and KRG-treated group (T+K). We measured testis weight and hormone levels and reactive oxygen species (ROS) from the left renal vein. Superoxide generation was measured on the basis of lucigenin-enhanced chemiluminescence in testis tissue.

Results: Testicular weight was significantly higher in the T+K group than in the T group; however, there were no significant differences in hormone levels between the 4 groups. The mean level of ROS and superoxide production was significantly higher in the T group than in the C group, whereas administration of KRG attenuated this increase. Upon histologic evaluation, the T group was found to have cellular disarray, a lack of cellular cohesiveness, degenerative changes in the germinal cells, and less distinct changes in the seminiferous tubules, whereas the T+K group had a germinal epithelial layer that appeared nearly normal.

Conclusions: The present study demonstrated that KRG recovered the testis dysfunction in the rat testis by suppressing superoxide production.

No MeSH data available.


Related in: MedlinePlus