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Effect of korean red ginseng on testicular tissue injury after torsion and detorsion.

Kim YH, Kim GH, Shin JH, Kim KS, Lim JS - Korean J Urol (2010)

Bottom Line: Testicular weight was significantly higher in the T+K group than in the T group; however, there were no significant differences in hormone levels between the 4 groups.The mean level of ROS and superoxide production was significantly higher in the T group than in the C group, whereas administration of KRG attenuated this increase.Upon histologic evaluation, the T group was found to have cellular disarray, a lack of cellular cohesiveness, degenerative changes in the germinal cells, and less distinct changes in the seminiferous tubules, whereas the T+K group had a germinal epithelial layer that appeared nearly normal.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Chungnam National University School of Medicine, Daejeon, Korea.

ABSTRACT

Purpose: Korean red ginseng (KRG) is a potent antioxidant and a free radical scavenger. This study was designed to determine whether KRG could protect against dysfunction and oxidative stress induced by torsion-detorsion injury in rat testis.

Materials and methods: Six-week-old male Sprague-Dawley rats were randomly divided into four groups: a sham-operated control group (C), a sham-operated and KRG-treated group (K), a 2 hours torsion and detorsion group (T), and a 2 hours torsion and detorsion and KRG-treated group (T+K). We measured testis weight and hormone levels and reactive oxygen species (ROS) from the left renal vein. Superoxide generation was measured on the basis of lucigenin-enhanced chemiluminescence in testis tissue.

Results: Testicular weight was significantly higher in the T+K group than in the T group; however, there were no significant differences in hormone levels between the 4 groups. The mean level of ROS and superoxide production was significantly higher in the T group than in the C group, whereas administration of KRG attenuated this increase. Upon histologic evaluation, the T group was found to have cellular disarray, a lack of cellular cohesiveness, degenerative changes in the germinal cells, and less distinct changes in the seminiferous tubules, whereas the T+K group had a germinal epithelial layer that appeared nearly normal.

Conclusions: The present study demonstrated that KRG recovered the testis dysfunction in the rat testis by suppressing superoxide production.

No MeSH data available.


Related in: MedlinePlus

Weight of the testis. C: a sham-operated group, K: a sham-operated and KRG-treated group, T: a 2 hours torsion and detorsion group, T+K: a 2 hours torsion and detorsion and KRG-treated group.
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Figure 1: Weight of the testis. C: a sham-operated group, K: a sham-operated and KRG-treated group, T: a 2 hours torsion and detorsion group, T+K: a 2 hours torsion and detorsion and KRG-treated group.

Mentions: The testicular weights of groups C and K were significantly different from the weights of groups T and T+K. In addition, although there were no significant differences in the testicular weights of groups C and K, the testicular weights of groups T and T+K differed significantly (Fig. 1).


Effect of korean red ginseng on testicular tissue injury after torsion and detorsion.

Kim YH, Kim GH, Shin JH, Kim KS, Lim JS - Korean J Urol (2010)

Weight of the testis. C: a sham-operated group, K: a sham-operated and KRG-treated group, T: a 2 hours torsion and detorsion group, T+K: a 2 hours torsion and detorsion and KRG-treated group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2991579&req=5

Figure 1: Weight of the testis. C: a sham-operated group, K: a sham-operated and KRG-treated group, T: a 2 hours torsion and detorsion group, T+K: a 2 hours torsion and detorsion and KRG-treated group.
Mentions: The testicular weights of groups C and K were significantly different from the weights of groups T and T+K. In addition, although there were no significant differences in the testicular weights of groups C and K, the testicular weights of groups T and T+K differed significantly (Fig. 1).

Bottom Line: Testicular weight was significantly higher in the T+K group than in the T group; however, there were no significant differences in hormone levels between the 4 groups.The mean level of ROS and superoxide production was significantly higher in the T group than in the C group, whereas administration of KRG attenuated this increase.Upon histologic evaluation, the T group was found to have cellular disarray, a lack of cellular cohesiveness, degenerative changes in the germinal cells, and less distinct changes in the seminiferous tubules, whereas the T+K group had a germinal epithelial layer that appeared nearly normal.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Chungnam National University School of Medicine, Daejeon, Korea.

ABSTRACT

Purpose: Korean red ginseng (KRG) is a potent antioxidant and a free radical scavenger. This study was designed to determine whether KRG could protect against dysfunction and oxidative stress induced by torsion-detorsion injury in rat testis.

Materials and methods: Six-week-old male Sprague-Dawley rats were randomly divided into four groups: a sham-operated control group (C), a sham-operated and KRG-treated group (K), a 2 hours torsion and detorsion group (T), and a 2 hours torsion and detorsion and KRG-treated group (T+K). We measured testis weight and hormone levels and reactive oxygen species (ROS) from the left renal vein. Superoxide generation was measured on the basis of lucigenin-enhanced chemiluminescence in testis tissue.

Results: Testicular weight was significantly higher in the T+K group than in the T group; however, there were no significant differences in hormone levels between the 4 groups. The mean level of ROS and superoxide production was significantly higher in the T group than in the C group, whereas administration of KRG attenuated this increase. Upon histologic evaluation, the T group was found to have cellular disarray, a lack of cellular cohesiveness, degenerative changes in the germinal cells, and less distinct changes in the seminiferous tubules, whereas the T+K group had a germinal epithelial layer that appeared nearly normal.

Conclusions: The present study demonstrated that KRG recovered the testis dysfunction in the rat testis by suppressing superoxide production.

No MeSH data available.


Related in: MedlinePlus