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Molecular characterization of Borrelia persica, the agent of tick borne relapsing fever in Israel and the Palestinian Authority.

Safdie G, Farrah IY, Yahia R, Marva E, Wilamowski A, Sawalha SS, Wald N, Schmiedel J, Moter A, Göbel UB, Bercovier H, Abdeen Z, Assous MV, Fishman Y - PLoS ONE (2010)

Bottom Line: In one sample we sequenced 7231 contiguous base pairs that covered completely the region from the 5'end of the 16S rRNA gene to the 5'end of the 23S rRNA gene comprising the whole 16S rRNA (rrs), and the following genes: Ala tRNA (alaT), Ile tRNA (ileT), adenylosuccinate lyase (purB), adenylosuccinate synthetase (purA), methylpurine-DNA glycosylase (mag), hypoxanthine-guanine phosphoribosyltransferase (hpt), an hydrolase (HAD superfamily) and a 135 bp 5' fragment of the 23S rRNA (rrlA) genes.Phylogenic sequence analysis defined all the Borrelia isolates from O. tholozani and from human TBRF cases in Israel and the West Bank as B. persica that clustered between the African and the New World TBRF species.Variants of B. persica were found among the different genes of the different isolates even in the same sampling area.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Genetics and Microbiology, Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem, Israel.

ABSTRACT
The identification of the Tick Borne Relapsing Fever (TBRF) agent in Israel and the Palestinian Authority relies on the morphology and the association of Borrelia persica with its vector Ornithodoros tholozani. Molecular based data on B. persica are very scarce as the organism is still non-cultivable. In this study, we were able to sequence three complete 16S rRNA genes, 12 partial flaB genes, 18 partial glpQ genes, 16 rrs-ileT intergenic spacers (IGS) from nine ticks and ten human blood samples originating from the West Bank and Israel. In one sample we sequenced 7231 contiguous base pairs that covered completely the region from the 5'end of the 16S rRNA gene to the 5'end of the 23S rRNA gene comprising the whole 16S rRNA (rrs), and the following genes: Ala tRNA (alaT), Ile tRNA (ileT), adenylosuccinate lyase (purB), adenylosuccinate synthetase (purA), methylpurine-DNA glycosylase (mag), hypoxanthine-guanine phosphoribosyltransferase (hpt), an hydrolase (HAD superfamily) and a 135 bp 5' fragment of the 23S rRNA (rrlA) genes. Phylogenic sequence analysis defined all the Borrelia isolates from O. tholozani and from human TBRF cases in Israel and the West Bank as B. persica that clustered between the African and the New World TBRF species. Gene organization of the intergenic spacer between the 16S rRNA and the 23S rRNA was similar to that of other TBRF Borrelia species and different from the Lyme disease Borrelia species. Variants of B. persica were found among the different genes of the different isolates even in the same sampling area.

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Related in: MedlinePlus

Phylogenetic tree based on the concatenated alignments of purA, glpQ, flaB and rrs-ileT IGS nucleotide sequences.The tree was inferred using the PHYML program. The percentage of trees in which the associated taxa clustered together in the bootstrap test (100 replicates) is shown next to the branches if higher than 80%. The tree was arbitrarily rooted at midpoint.
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pone-0014105-g007: Phylogenetic tree based on the concatenated alignments of purA, glpQ, flaB and rrs-ileT IGS nucleotide sequences.The tree was inferred using the PHYML program. The percentage of trees in which the associated taxa clustered together in the bootstrap test (100 replicates) is shown next to the branches if higher than 80%. The tree was arbitrarily rooted at midpoint.

Mentions: To definitively confirm the speciation of the Israeli and Palestinian authority Borrelia isolates, MLSA was performed on seven isolates originating from human and tick samples and belonging to both 16S rRNA genovars (Figure 7). The MLSA study was based on genes of different loci both coding (purA, flaB and glpQ) and the non-coding (rrs-ileT IGS) sequences. The alignment of the sequences for each of the loci reveals a low level of diversity among the isolates (Table 4). A concatenated sequence based on these genes was used for a phylogenic analysis with homologous loci of other TBRF Borrelia species. The seven independent local isolates formed a unique cluster separated from all other species (Figure 7).


Molecular characterization of Borrelia persica, the agent of tick borne relapsing fever in Israel and the Palestinian Authority.

Safdie G, Farrah IY, Yahia R, Marva E, Wilamowski A, Sawalha SS, Wald N, Schmiedel J, Moter A, Göbel UB, Bercovier H, Abdeen Z, Assous MV, Fishman Y - PLoS ONE (2010)

Phylogenetic tree based on the concatenated alignments of purA, glpQ, flaB and rrs-ileT IGS nucleotide sequences.The tree was inferred using the PHYML program. The percentage of trees in which the associated taxa clustered together in the bootstrap test (100 replicates) is shown next to the branches if higher than 80%. The tree was arbitrarily rooted at midpoint.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2991353&req=5

pone-0014105-g007: Phylogenetic tree based on the concatenated alignments of purA, glpQ, flaB and rrs-ileT IGS nucleotide sequences.The tree was inferred using the PHYML program. The percentage of trees in which the associated taxa clustered together in the bootstrap test (100 replicates) is shown next to the branches if higher than 80%. The tree was arbitrarily rooted at midpoint.
Mentions: To definitively confirm the speciation of the Israeli and Palestinian authority Borrelia isolates, MLSA was performed on seven isolates originating from human and tick samples and belonging to both 16S rRNA genovars (Figure 7). The MLSA study was based on genes of different loci both coding (purA, flaB and glpQ) and the non-coding (rrs-ileT IGS) sequences. The alignment of the sequences for each of the loci reveals a low level of diversity among the isolates (Table 4). A concatenated sequence based on these genes was used for a phylogenic analysis with homologous loci of other TBRF Borrelia species. The seven independent local isolates formed a unique cluster separated from all other species (Figure 7).

Bottom Line: In one sample we sequenced 7231 contiguous base pairs that covered completely the region from the 5'end of the 16S rRNA gene to the 5'end of the 23S rRNA gene comprising the whole 16S rRNA (rrs), and the following genes: Ala tRNA (alaT), Ile tRNA (ileT), adenylosuccinate lyase (purB), adenylosuccinate synthetase (purA), methylpurine-DNA glycosylase (mag), hypoxanthine-guanine phosphoribosyltransferase (hpt), an hydrolase (HAD superfamily) and a 135 bp 5' fragment of the 23S rRNA (rrlA) genes.Phylogenic sequence analysis defined all the Borrelia isolates from O. tholozani and from human TBRF cases in Israel and the West Bank as B. persica that clustered between the African and the New World TBRF species.Variants of B. persica were found among the different genes of the different isolates even in the same sampling area.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Genetics and Microbiology, Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem, Israel.

ABSTRACT
The identification of the Tick Borne Relapsing Fever (TBRF) agent in Israel and the Palestinian Authority relies on the morphology and the association of Borrelia persica with its vector Ornithodoros tholozani. Molecular based data on B. persica are very scarce as the organism is still non-cultivable. In this study, we were able to sequence three complete 16S rRNA genes, 12 partial flaB genes, 18 partial glpQ genes, 16 rrs-ileT intergenic spacers (IGS) from nine ticks and ten human blood samples originating from the West Bank and Israel. In one sample we sequenced 7231 contiguous base pairs that covered completely the region from the 5'end of the 16S rRNA gene to the 5'end of the 23S rRNA gene comprising the whole 16S rRNA (rrs), and the following genes: Ala tRNA (alaT), Ile tRNA (ileT), adenylosuccinate lyase (purB), adenylosuccinate synthetase (purA), methylpurine-DNA glycosylase (mag), hypoxanthine-guanine phosphoribosyltransferase (hpt), an hydrolase (HAD superfamily) and a 135 bp 5' fragment of the 23S rRNA (rrlA) genes. Phylogenic sequence analysis defined all the Borrelia isolates from O. tholozani and from human TBRF cases in Israel and the West Bank as B. persica that clustered between the African and the New World TBRF species. Gene organization of the intergenic spacer between the 16S rRNA and the 23S rRNA was similar to that of other TBRF Borrelia species and different from the Lyme disease Borrelia species. Variants of B. persica were found among the different genes of the different isolates even in the same sampling area.

Show MeSH
Related in: MedlinePlus