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Molecular characterization of Borrelia persica, the agent of tick borne relapsing fever in Israel and the Palestinian Authority.

Safdie G, Farrah IY, Yahia R, Marva E, Wilamowski A, Sawalha SS, Wald N, Schmiedel J, Moter A, Göbel UB, Bercovier H, Abdeen Z, Assous MV, Fishman Y - PLoS ONE (2010)

Bottom Line: In one sample we sequenced 7231 contiguous base pairs that covered completely the region from the 5'end of the 16S rRNA gene to the 5'end of the 23S rRNA gene comprising the whole 16S rRNA (rrs), and the following genes: Ala tRNA (alaT), Ile tRNA (ileT), adenylosuccinate lyase (purB), adenylosuccinate synthetase (purA), methylpurine-DNA glycosylase (mag), hypoxanthine-guanine phosphoribosyltransferase (hpt), an hydrolase (HAD superfamily) and a 135 bp 5' fragment of the 23S rRNA (rrlA) genes.Phylogenic sequence analysis defined all the Borrelia isolates from O. tholozani and from human TBRF cases in Israel and the West Bank as B. persica that clustered between the African and the New World TBRF species.Variants of B. persica were found among the different genes of the different isolates even in the same sampling area.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Genetics and Microbiology, Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem, Israel.

ABSTRACT
The identification of the Tick Borne Relapsing Fever (TBRF) agent in Israel and the Palestinian Authority relies on the morphology and the association of Borrelia persica with its vector Ornithodoros tholozani. Molecular based data on B. persica are very scarce as the organism is still non-cultivable. In this study, we were able to sequence three complete 16S rRNA genes, 12 partial flaB genes, 18 partial glpQ genes, 16 rrs-ileT intergenic spacers (IGS) from nine ticks and ten human blood samples originating from the West Bank and Israel. In one sample we sequenced 7231 contiguous base pairs that covered completely the region from the 5'end of the 16S rRNA gene to the 5'end of the 23S rRNA gene comprising the whole 16S rRNA (rrs), and the following genes: Ala tRNA (alaT), Ile tRNA (ileT), adenylosuccinate lyase (purB), adenylosuccinate synthetase (purA), methylpurine-DNA glycosylase (mag), hypoxanthine-guanine phosphoribosyltransferase (hpt), an hydrolase (HAD superfamily) and a 135 bp 5' fragment of the 23S rRNA (rrlA) genes. Phylogenic sequence analysis defined all the Borrelia isolates from O. tholozani and from human TBRF cases in Israel and the West Bank as B. persica that clustered between the African and the New World TBRF species. Gene organization of the intergenic spacer between the 16S rRNA and the 23S rRNA was similar to that of other TBRF Borrelia species and different from the Lyme disease Borrelia species. Variants of B. persica were found among the different genes of the different isolates even in the same sampling area.

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Related in: MedlinePlus

Phylogenetic tree based on purA nucleotide sequences.The complete purA sequences of B. persica isolates in Israel and the West Bank were compared to purA sequences from other Borrelia species (accession number are given in parentheses). The Phylogenic tree was inferred using the UPGMA method. Parameters were as described in Figure 2. All positions containing gaps and missing data were eliminated from the dataset (Complete deletion option). There were a total of 1284 positions in the final dataset.
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pone-0014105-g005: Phylogenetic tree based on purA nucleotide sequences.The complete purA sequences of B. persica isolates in Israel and the West Bank were compared to purA sequences from other Borrelia species (accession number are given in parentheses). The Phylogenic tree was inferred using the UPGMA method. Parameters were as described in Figure 2. All positions containing gaps and missing data were eliminated from the dataset (Complete deletion option). There were a total of 1284 positions in the final dataset.

Mentions: Among the genes in the large 7231 bp contig, the hypoxanthine-guanine phosphoribosyltransferase (hpt), adenylosuccinate lyase (purB) and adenylosuccinate synthase (purA) belong to a group of six open reading frames found in the TBRF Borrelia species but not in B. burgdorferi [24]. The 1284 bp sequence of the purA gene was amplified and sequenced. Analysis of sequences from eight B. persica isolates, seven from human hosts and one from O. tholozani revealed the presence of 4 genovars reflecting five base pair substitutions, two of which result in amino acid substitutions Ser to Leu in genovar P3 and Ser to Pro in genovar P4) (Table 6). The data obtained from the analysis of purA sequences confirmed that all the local strains belonged to a single phylogenetic cluster (Figure 5).


Molecular characterization of Borrelia persica, the agent of tick borne relapsing fever in Israel and the Palestinian Authority.

Safdie G, Farrah IY, Yahia R, Marva E, Wilamowski A, Sawalha SS, Wald N, Schmiedel J, Moter A, Göbel UB, Bercovier H, Abdeen Z, Assous MV, Fishman Y - PLoS ONE (2010)

Phylogenetic tree based on purA nucleotide sequences.The complete purA sequences of B. persica isolates in Israel and the West Bank were compared to purA sequences from other Borrelia species (accession number are given in parentheses). The Phylogenic tree was inferred using the UPGMA method. Parameters were as described in Figure 2. All positions containing gaps and missing data were eliminated from the dataset (Complete deletion option). There were a total of 1284 positions in the final dataset.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2991353&req=5

pone-0014105-g005: Phylogenetic tree based on purA nucleotide sequences.The complete purA sequences of B. persica isolates in Israel and the West Bank were compared to purA sequences from other Borrelia species (accession number are given in parentheses). The Phylogenic tree was inferred using the UPGMA method. Parameters were as described in Figure 2. All positions containing gaps and missing data were eliminated from the dataset (Complete deletion option). There were a total of 1284 positions in the final dataset.
Mentions: Among the genes in the large 7231 bp contig, the hypoxanthine-guanine phosphoribosyltransferase (hpt), adenylosuccinate lyase (purB) and adenylosuccinate synthase (purA) belong to a group of six open reading frames found in the TBRF Borrelia species but not in B. burgdorferi [24]. The 1284 bp sequence of the purA gene was amplified and sequenced. Analysis of sequences from eight B. persica isolates, seven from human hosts and one from O. tholozani revealed the presence of 4 genovars reflecting five base pair substitutions, two of which result in amino acid substitutions Ser to Leu in genovar P3 and Ser to Pro in genovar P4) (Table 6). The data obtained from the analysis of purA sequences confirmed that all the local strains belonged to a single phylogenetic cluster (Figure 5).

Bottom Line: In one sample we sequenced 7231 contiguous base pairs that covered completely the region from the 5'end of the 16S rRNA gene to the 5'end of the 23S rRNA gene comprising the whole 16S rRNA (rrs), and the following genes: Ala tRNA (alaT), Ile tRNA (ileT), adenylosuccinate lyase (purB), adenylosuccinate synthetase (purA), methylpurine-DNA glycosylase (mag), hypoxanthine-guanine phosphoribosyltransferase (hpt), an hydrolase (HAD superfamily) and a 135 bp 5' fragment of the 23S rRNA (rrlA) genes.Phylogenic sequence analysis defined all the Borrelia isolates from O. tholozani and from human TBRF cases in Israel and the West Bank as B. persica that clustered between the African and the New World TBRF species.Variants of B. persica were found among the different genes of the different isolates even in the same sampling area.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Genetics and Microbiology, Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem, Israel.

ABSTRACT
The identification of the Tick Borne Relapsing Fever (TBRF) agent in Israel and the Palestinian Authority relies on the morphology and the association of Borrelia persica with its vector Ornithodoros tholozani. Molecular based data on B. persica are very scarce as the organism is still non-cultivable. In this study, we were able to sequence three complete 16S rRNA genes, 12 partial flaB genes, 18 partial glpQ genes, 16 rrs-ileT intergenic spacers (IGS) from nine ticks and ten human blood samples originating from the West Bank and Israel. In one sample we sequenced 7231 contiguous base pairs that covered completely the region from the 5'end of the 16S rRNA gene to the 5'end of the 23S rRNA gene comprising the whole 16S rRNA (rrs), and the following genes: Ala tRNA (alaT), Ile tRNA (ileT), adenylosuccinate lyase (purB), adenylosuccinate synthetase (purA), methylpurine-DNA glycosylase (mag), hypoxanthine-guanine phosphoribosyltransferase (hpt), an hydrolase (HAD superfamily) and a 135 bp 5' fragment of the 23S rRNA (rrlA) genes. Phylogenic sequence analysis defined all the Borrelia isolates from O. tholozani and from human TBRF cases in Israel and the West Bank as B. persica that clustered between the African and the New World TBRF species. Gene organization of the intergenic spacer between the 16S rRNA and the 23S rRNA was similar to that of other TBRF Borrelia species and different from the Lyme disease Borrelia species. Variants of B. persica were found among the different genes of the different isolates even in the same sampling area.

Show MeSH
Related in: MedlinePlus