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Molecular characterization of Borrelia persica, the agent of tick borne relapsing fever in Israel and the Palestinian Authority.

Safdie G, Farrah IY, Yahia R, Marva E, Wilamowski A, Sawalha SS, Wald N, Schmiedel J, Moter A, Göbel UB, Bercovier H, Abdeen Z, Assous MV, Fishman Y - PLoS ONE (2010)

Bottom Line: In one sample we sequenced 7231 contiguous base pairs that covered completely the region from the 5'end of the 16S rRNA gene to the 5'end of the 23S rRNA gene comprising the whole 16S rRNA (rrs), and the following genes: Ala tRNA (alaT), Ile tRNA (ileT), adenylosuccinate lyase (purB), adenylosuccinate synthetase (purA), methylpurine-DNA glycosylase (mag), hypoxanthine-guanine phosphoribosyltransferase (hpt), an hydrolase (HAD superfamily) and a 135 bp 5' fragment of the 23S rRNA (rrlA) genes.Phylogenic sequence analysis defined all the Borrelia isolates from O. tholozani and from human TBRF cases in Israel and the West Bank as B. persica that clustered between the African and the New World TBRF species.Variants of B. persica were found among the different genes of the different isolates even in the same sampling area.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Genetics and Microbiology, Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem, Israel.

ABSTRACT
The identification of the Tick Borne Relapsing Fever (TBRF) agent in Israel and the Palestinian Authority relies on the morphology and the association of Borrelia persica with its vector Ornithodoros tholozani. Molecular based data on B. persica are very scarce as the organism is still non-cultivable. In this study, we were able to sequence three complete 16S rRNA genes, 12 partial flaB genes, 18 partial glpQ genes, 16 rrs-ileT intergenic spacers (IGS) from nine ticks and ten human blood samples originating from the West Bank and Israel. In one sample we sequenced 7231 contiguous base pairs that covered completely the region from the 5'end of the 16S rRNA gene to the 5'end of the 23S rRNA gene comprising the whole 16S rRNA (rrs), and the following genes: Ala tRNA (alaT), Ile tRNA (ileT), adenylosuccinate lyase (purB), adenylosuccinate synthetase (purA), methylpurine-DNA glycosylase (mag), hypoxanthine-guanine phosphoribosyltransferase (hpt), an hydrolase (HAD superfamily) and a 135 bp 5' fragment of the 23S rRNA (rrlA) genes. Phylogenic sequence analysis defined all the Borrelia isolates from O. tholozani and from human TBRF cases in Israel and the West Bank as B. persica that clustered between the African and the New World TBRF species. Gene organization of the intergenic spacer between the 16S rRNA and the 23S rRNA was similar to that of other TBRF Borrelia species and different from the Lyme disease Borrelia species. Variants of B. persica were found among the different genes of the different isolates even in the same sampling area.

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Related in: MedlinePlus

Phylogenic tree based on rrs sequences.The complete rrs sequences of B. persica isolates in Israel were compared to rrs sequences from B. persica (Iran) and other Borrelia species (accession numbers are given in parentheses). The phylogenic tree was inferred using the UPGMA method. Parameters were as described in Figure 2. All positions containing gaps and missing data were eliminated from the dataset (Complete deletion option). There were a total of 1522 positions in the final dataset.
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pone-0014105-g004: Phylogenic tree based on rrs sequences.The complete rrs sequences of B. persica isolates in Israel were compared to rrs sequences from B. persica (Iran) and other Borrelia species (accession numbers are given in parentheses). The phylogenic tree was inferred using the UPGMA method. Parameters were as described in Figure 2. All positions containing gaps and missing data were eliminated from the dataset (Complete deletion option). There were a total of 1522 positions in the final dataset.

Mentions: The relationship among the TBRF species is also reflected in the phylogenic tree presented in Figure 4. The analyzed Borrelia species were separated into two clusters, one including the New World species B. turicatae and B. hermsii and, the second dividing into two branches that included on the one hand the African strains B. hispanica, B. duttonii and B. recurrentis and on the other hand all Israeli isolates that grouped together with the reference Iranian B. persica strain.


Molecular characterization of Borrelia persica, the agent of tick borne relapsing fever in Israel and the Palestinian Authority.

Safdie G, Farrah IY, Yahia R, Marva E, Wilamowski A, Sawalha SS, Wald N, Schmiedel J, Moter A, Göbel UB, Bercovier H, Abdeen Z, Assous MV, Fishman Y - PLoS ONE (2010)

Phylogenic tree based on rrs sequences.The complete rrs sequences of B. persica isolates in Israel were compared to rrs sequences from B. persica (Iran) and other Borrelia species (accession numbers are given in parentheses). The phylogenic tree was inferred using the UPGMA method. Parameters were as described in Figure 2. All positions containing gaps and missing data were eliminated from the dataset (Complete deletion option). There were a total of 1522 positions in the final dataset.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2991353&req=5

pone-0014105-g004: Phylogenic tree based on rrs sequences.The complete rrs sequences of B. persica isolates in Israel were compared to rrs sequences from B. persica (Iran) and other Borrelia species (accession numbers are given in parentheses). The phylogenic tree was inferred using the UPGMA method. Parameters were as described in Figure 2. All positions containing gaps and missing data were eliminated from the dataset (Complete deletion option). There were a total of 1522 positions in the final dataset.
Mentions: The relationship among the TBRF species is also reflected in the phylogenic tree presented in Figure 4. The analyzed Borrelia species were separated into two clusters, one including the New World species B. turicatae and B. hermsii and, the second dividing into two branches that included on the one hand the African strains B. hispanica, B. duttonii and B. recurrentis and on the other hand all Israeli isolates that grouped together with the reference Iranian B. persica strain.

Bottom Line: In one sample we sequenced 7231 contiguous base pairs that covered completely the region from the 5'end of the 16S rRNA gene to the 5'end of the 23S rRNA gene comprising the whole 16S rRNA (rrs), and the following genes: Ala tRNA (alaT), Ile tRNA (ileT), adenylosuccinate lyase (purB), adenylosuccinate synthetase (purA), methylpurine-DNA glycosylase (mag), hypoxanthine-guanine phosphoribosyltransferase (hpt), an hydrolase (HAD superfamily) and a 135 bp 5' fragment of the 23S rRNA (rrlA) genes.Phylogenic sequence analysis defined all the Borrelia isolates from O. tholozani and from human TBRF cases in Israel and the West Bank as B. persica that clustered between the African and the New World TBRF species.Variants of B. persica were found among the different genes of the different isolates even in the same sampling area.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Genetics and Microbiology, Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem, Israel.

ABSTRACT
The identification of the Tick Borne Relapsing Fever (TBRF) agent in Israel and the Palestinian Authority relies on the morphology and the association of Borrelia persica with its vector Ornithodoros tholozani. Molecular based data on B. persica are very scarce as the organism is still non-cultivable. In this study, we were able to sequence three complete 16S rRNA genes, 12 partial flaB genes, 18 partial glpQ genes, 16 rrs-ileT intergenic spacers (IGS) from nine ticks and ten human blood samples originating from the West Bank and Israel. In one sample we sequenced 7231 contiguous base pairs that covered completely the region from the 5'end of the 16S rRNA gene to the 5'end of the 23S rRNA gene comprising the whole 16S rRNA (rrs), and the following genes: Ala tRNA (alaT), Ile tRNA (ileT), adenylosuccinate lyase (purB), adenylosuccinate synthetase (purA), methylpurine-DNA glycosylase (mag), hypoxanthine-guanine phosphoribosyltransferase (hpt), an hydrolase (HAD superfamily) and a 135 bp 5' fragment of the 23S rRNA (rrlA) genes. Phylogenic sequence analysis defined all the Borrelia isolates from O. tholozani and from human TBRF cases in Israel and the West Bank as B. persica that clustered between the African and the New World TBRF species. Gene organization of the intergenic spacer between the 16S rRNA and the 23S rRNA was similar to that of other TBRF Borrelia species and different from the Lyme disease Borrelia species. Variants of B. persica were found among the different genes of the different isolates even in the same sampling area.

Show MeSH
Related in: MedlinePlus