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Pulmonary exposure to carbon black nanoparticles and vascular effects.

Vesterdal LK, Folkmann JK, Jacobsen NR, Sheykhzade M, Wallin H, Loft S, Møller P - Part Fibre Toxicol (2010)

Bottom Line: Exposure to small size particulates is regarded as a risk factor for cardiovascular diseases.Exposure to nano-sized carbon black particles is associated with modest vasomotor impairment, which is associated neither with nitrosative stress nor with any obvious increases in the expression of cell adhesion proteins on endothelial cells or in plaque progression.Evidence of pulmonary inflammation was observed, but only in animals exposed to higher doses.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Public Health, Section of Environmental Health, University of Copenhagen, Copenhagen, Denmark.

ABSTRACT

Background: Exposure to small size particulates is regarded as a risk factor for cardiovascular diseases.

Methods: We exposed young and aged apolipoprotein E knockout mice (apoE-/-) to carbon black (Printex 90, 14 nm) by intratracheal instillation, with different dosing and timing, and measured vasomotor function, progression of atherosclerotic plaques, and VCAM-1, ICAM-1, and 3-nitrotyrosine in blood vessels. The mRNA expression of VCAM-1, ICAM-1, HO-1, and MCP-1 was examined in lung tissue.

Results: Young apoE-/- mice exposed to two consecutive 0.5 mg/kg doses of carbon black exhibited lower acetylcholine-induced vasorelaxation in aorta segments mounted in myographs, whereas single doses of 0.05-2.7 mg/kg produced no such effects. The phenylephrine-dependent vasocontraction response was shifted toward a lower responsiveness in the mice exposed once to a low dose for 24 hours. No effects were seen on the progression of atherosclerotic plaques in the aged apoE-/- mice or on the expression of VCAM-1 and ICAM-1 and the presence of 3-nitrotyrosine in the vascular tissue of either young or aged apoE-/- mice. The expression of MCP-1 mRNA was increased in the lungs of young apoE-/- mice exposed to 0.9-2.7 mg/kg carbon black for 24 hours and of aged apoE-/- mice exposed to two consecutive 0.5 mg/kg doses of carbon black seven and five weeks prior to sacrifice.

Conclusion: Exposure to nano-sized carbon black particles is associated with modest vasomotor impairment, which is associated neither with nitrosative stress nor with any obvious increases in the expression of cell adhesion proteins on endothelial cells or in plaque progression. Evidence of pulmonary inflammation was observed, but only in animals exposed to higher doses.

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Expression of VCAM-1 and ICAM-1 on the endothelium and the presence of 3-nitrotyrosine (3NT) in the vascular tissue of aorta from 11-13 weeks old apoE-/- mice exposed to carbon black by i.t. instillation. The antibodies used were HRP-streptavidin conjugated and visualized with DAB. On the left, the data are depicted as geometric means of ranked values representing the amount of staining assessed in 4 sections of aorta from each animal. The medians are depicted as horizontal lines (n = 10-20); each symbol represent the result from one animal. On the right, images are shown of aorta sections representative of the median in the control group (left) and the 2.7 mg/kg exposed group (right). Binding of antibodies can be seen as brown staining of the tissue.
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Figure 5: Expression of VCAM-1 and ICAM-1 on the endothelium and the presence of 3-nitrotyrosine (3NT) in the vascular tissue of aorta from 11-13 weeks old apoE-/- mice exposed to carbon black by i.t. instillation. The antibodies used were HRP-streptavidin conjugated and visualized with DAB. On the left, the data are depicted as geometric means of ranked values representing the amount of staining assessed in 4 sections of aorta from each animal. The medians are depicted as horizontal lines (n = 10-20); each symbol represent the result from one animal. On the right, images are shown of aorta sections representative of the median in the control group (left) and the 2.7 mg/kg exposed group (right). Binding of antibodies can be seen as brown staining of the tissue.

Mentions: VCAM-1, ICAM-1, and 3-nitrotyrosine was visualized by immunohistochemistry in aorta sections of young apoE-/- mice exposed to either 0.9 or 2.7 mg/kg CB or vehicle 24 hours prior to sacrifice. No significant effect of CB exposure on the expression of the surface proteins or on the presence of 3-nitrotyrosine could be observed when compared to the control group (Figure 5a-c).


Pulmonary exposure to carbon black nanoparticles and vascular effects.

Vesterdal LK, Folkmann JK, Jacobsen NR, Sheykhzade M, Wallin H, Loft S, Møller P - Part Fibre Toxicol (2010)

Expression of VCAM-1 and ICAM-1 on the endothelium and the presence of 3-nitrotyrosine (3NT) in the vascular tissue of aorta from 11-13 weeks old apoE-/- mice exposed to carbon black by i.t. instillation. The antibodies used were HRP-streptavidin conjugated and visualized with DAB. On the left, the data are depicted as geometric means of ranked values representing the amount of staining assessed in 4 sections of aorta from each animal. The medians are depicted as horizontal lines (n = 10-20); each symbol represent the result from one animal. On the right, images are shown of aorta sections representative of the median in the control group (left) and the 2.7 mg/kg exposed group (right). Binding of antibodies can be seen as brown staining of the tissue.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2991279&req=5

Figure 5: Expression of VCAM-1 and ICAM-1 on the endothelium and the presence of 3-nitrotyrosine (3NT) in the vascular tissue of aorta from 11-13 weeks old apoE-/- mice exposed to carbon black by i.t. instillation. The antibodies used were HRP-streptavidin conjugated and visualized with DAB. On the left, the data are depicted as geometric means of ranked values representing the amount of staining assessed in 4 sections of aorta from each animal. The medians are depicted as horizontal lines (n = 10-20); each symbol represent the result from one animal. On the right, images are shown of aorta sections representative of the median in the control group (left) and the 2.7 mg/kg exposed group (right). Binding of antibodies can be seen as brown staining of the tissue.
Mentions: VCAM-1, ICAM-1, and 3-nitrotyrosine was visualized by immunohistochemistry in aorta sections of young apoE-/- mice exposed to either 0.9 or 2.7 mg/kg CB or vehicle 24 hours prior to sacrifice. No significant effect of CB exposure on the expression of the surface proteins or on the presence of 3-nitrotyrosine could be observed when compared to the control group (Figure 5a-c).

Bottom Line: Exposure to small size particulates is regarded as a risk factor for cardiovascular diseases.Exposure to nano-sized carbon black particles is associated with modest vasomotor impairment, which is associated neither with nitrosative stress nor with any obvious increases in the expression of cell adhesion proteins on endothelial cells or in plaque progression.Evidence of pulmonary inflammation was observed, but only in animals exposed to higher doses.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Public Health, Section of Environmental Health, University of Copenhagen, Copenhagen, Denmark.

ABSTRACT

Background: Exposure to small size particulates is regarded as a risk factor for cardiovascular diseases.

Methods: We exposed young and aged apolipoprotein E knockout mice (apoE-/-) to carbon black (Printex 90, 14 nm) by intratracheal instillation, with different dosing and timing, and measured vasomotor function, progression of atherosclerotic plaques, and VCAM-1, ICAM-1, and 3-nitrotyrosine in blood vessels. The mRNA expression of VCAM-1, ICAM-1, HO-1, and MCP-1 was examined in lung tissue.

Results: Young apoE-/- mice exposed to two consecutive 0.5 mg/kg doses of carbon black exhibited lower acetylcholine-induced vasorelaxation in aorta segments mounted in myographs, whereas single doses of 0.05-2.7 mg/kg produced no such effects. The phenylephrine-dependent vasocontraction response was shifted toward a lower responsiveness in the mice exposed once to a low dose for 24 hours. No effects were seen on the progression of atherosclerotic plaques in the aged apoE-/- mice or on the expression of VCAM-1 and ICAM-1 and the presence of 3-nitrotyrosine in the vascular tissue of either young or aged apoE-/- mice. The expression of MCP-1 mRNA was increased in the lungs of young apoE-/- mice exposed to 0.9-2.7 mg/kg carbon black for 24 hours and of aged apoE-/- mice exposed to two consecutive 0.5 mg/kg doses of carbon black seven and five weeks prior to sacrifice.

Conclusion: Exposure to nano-sized carbon black particles is associated with modest vasomotor impairment, which is associated neither with nitrosative stress nor with any obvious increases in the expression of cell adhesion proteins on endothelial cells or in plaque progression. Evidence of pulmonary inflammation was observed, but only in animals exposed to higher doses.

Show MeSH
Related in: MedlinePlus