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Biocompatible Polyhydroxyethylaspartamide-based Micelles with Gadolinium for MRI Contrast Agents.

Jeong SY, Kim HJ, Kwak BK, Lee HY, Seong H, Shin BC, Yuk SH, Hwang SJ, Cho SH - Nanoscale Res Lett (2010)

Bottom Line: All the synthesized materials were characterized by proton nuclear magnetic resonance ((1)H NMR).Micelles with PHEA-mPEG-C(16)-ED-DOTA-Gd showed higher relaxivities than the commercially available gadolinium contrast agent.Moreover, the signal intensity of a rabbit liver was effectively increased after intravenous injection of PHEA-mPEG-C(16)-ED-DOTA-Gd.

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ABSTRACT
Biocompatible poly-[N-(2-hydroxyethyl)-d,l-aspartamide]-methoxypoly(ethyleneglycol)-hexadecylamine (PHEA-mPEG-C(16)) conjugated with 1,4,7,10-tetraazacyclododecan-1,4,7,10-tetraacetic acid-gadolinium (DOTA-Gd) via ethylenediamine (ED) was synthesized as a magnetic resonance imaging (MRI) contrast agent. Amphiphilic PHEA-mPEG-C(16)-ED-DOTA-Gd forms micelle in aqueous solution. All the synthesized materials were characterized by proton nuclear magnetic resonance ((1)H NMR). Micelle size and shape were examined by dynamic light scattering (DLS) and atomic force microscopy (AFM). Micelles with PHEA-mPEG-C(16)-ED-DOTA-Gd showed higher relaxivities than the commercially available gadolinium contrast agent. Moreover, the signal intensity of a rabbit liver was effectively increased after intravenous injection of PHEA-mPEG-C(16)-ED-DOTA-Gd.

No MeSH data available.


a ELS data and b AFM image of PHEA-mPEG-C16-ED micelles; average size, 180 nm
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Figure 3: a ELS data and b AFM image of PHEA-mPEG-C16-ED micelles; average size, 180 nm

Mentions: DOTA was conjugated to PHEA-mPEG-C16-ED using EDC/NHS, and then Gd was chelated to PHEA-mPEG-C16-ED-DOTA. Gd in PHEA-mPEG-C16-ED-DOTA-Gd was confirmed by EDS analysis (Fig. 2). Table 2 showed that content of C, H, and N was decreased after incorporation of Gd. The average diameter of prepared micellar PHEA-mPEG-C16-ED-DOTA was 180 nm from the data of ELS and AFM (Fig. 3). That size is suitable for uptake in liver.


Biocompatible Polyhydroxyethylaspartamide-based Micelles with Gadolinium for MRI Contrast Agents.

Jeong SY, Kim HJ, Kwak BK, Lee HY, Seong H, Shin BC, Yuk SH, Hwang SJ, Cho SH - Nanoscale Res Lett (2010)

a ELS data and b AFM image of PHEA-mPEG-C16-ED micelles; average size, 180 nm
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2991228&req=5

Figure 3: a ELS data and b AFM image of PHEA-mPEG-C16-ED micelles; average size, 180 nm
Mentions: DOTA was conjugated to PHEA-mPEG-C16-ED using EDC/NHS, and then Gd was chelated to PHEA-mPEG-C16-ED-DOTA. Gd in PHEA-mPEG-C16-ED-DOTA-Gd was confirmed by EDS analysis (Fig. 2). Table 2 showed that content of C, H, and N was decreased after incorporation of Gd. The average diameter of prepared micellar PHEA-mPEG-C16-ED-DOTA was 180 nm from the data of ELS and AFM (Fig. 3). That size is suitable for uptake in liver.

Bottom Line: All the synthesized materials were characterized by proton nuclear magnetic resonance ((1)H NMR).Micelles with PHEA-mPEG-C(16)-ED-DOTA-Gd showed higher relaxivities than the commercially available gadolinium contrast agent.Moreover, the signal intensity of a rabbit liver was effectively increased after intravenous injection of PHEA-mPEG-C(16)-ED-DOTA-Gd.

View Article: PubMed Central - HTML - PubMed

ABSTRACT
Biocompatible poly-[N-(2-hydroxyethyl)-d,l-aspartamide]-methoxypoly(ethyleneglycol)-hexadecylamine (PHEA-mPEG-C(16)) conjugated with 1,4,7,10-tetraazacyclododecan-1,4,7,10-tetraacetic acid-gadolinium (DOTA-Gd) via ethylenediamine (ED) was synthesized as a magnetic resonance imaging (MRI) contrast agent. Amphiphilic PHEA-mPEG-C(16)-ED-DOTA-Gd forms micelle in aqueous solution. All the synthesized materials were characterized by proton nuclear magnetic resonance ((1)H NMR). Micelle size and shape were examined by dynamic light scattering (DLS) and atomic force microscopy (AFM). Micelles with PHEA-mPEG-C(16)-ED-DOTA-Gd showed higher relaxivities than the commercially available gadolinium contrast agent. Moreover, the signal intensity of a rabbit liver was effectively increased after intravenous injection of PHEA-mPEG-C(16)-ED-DOTA-Gd.

No MeSH data available.