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Pulvinar projections to the striatum and amygdala in the tree shrew.

Day-Brown JD, Wei H, Chomsung RD, Petry HM, Bickford ME - Front Neuroanat (2010)

Bottom Line: Both capabilities are thought to be mediated by pathways from the retina through the superior colliculus (SC) and pulvinar nucleus.Using immunocytochemical staining for substance P (SP) and parvalbumin (PV) to reveal the patch/matrix organization of tree shrew striatum, we found that SP-rich/PV-poor patches interlock with a PV-rich/SP-poor matrix.Electron microscopy revealed that the postsynaptic targets of tracer-labeled pulvino-striatal and pulvino-amygdala terminals are spines, demonstrating that the pulvinar nucleus projects to the spiny output cells of the striatum matrix and the lateral amygdala, potentially relaying: (1) topographic visual information from SC to striatum to aid in guiding precise movements, and (2) non-topographic visual information from SC to the amygdala alerting the animal to potentially dangerous visual images.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomical Sciences and Neurobiology, University of Louisville Medical Center Louisville, KY, USA.

ABSTRACT
Visually guided movement is possible in the absence of conscious visual perception, a phenomenon referred to as "blindsight." Similarly, fearful images can elicit emotional responses in the absence of their conscious perception. Both capabilities are thought to be mediated by pathways from the retina through the superior colliculus (SC) and pulvinar nucleus. To define potential pathways that underlie behavioral responses to unperceived visual stimuli, we examined the projections from the pulvinar nucleus to the striatum and amygdala in the tree shrew (Tupaia belangeri), a species considered to be a prototypical primate. The tree shrew brain has a large pulvinar nucleus that contains two SC-recipient subdivisions; the dorsal (Pd) and central (Pc) pulvinar both receive topographic ("specific") projections from SC, and Pd receives an additional non-topographic ("diffuse") projection from SC (Chomsung et al., 2008). Anterograde and retrograde tract tracing revealed that both Pd and Pc project to the caudate and putamen, and Pd, but not Pc, additionally projects to the lateral amygdala. Using immunocytochemical staining for substance P (SP) and parvalbumin (PV) to reveal the patch/matrix organization of tree shrew striatum, we found that SP-rich/PV-poor patches interlock with a PV-rich/SP-poor matrix. Confocal microscopy revealed that tracer-labeled pulvino-striatal terminals preferentially innervate the matrix. Electron microscopy revealed that the postsynaptic targets of tracer-labeled pulvino-striatal and pulvino-amygdala terminals are spines, demonstrating that the pulvinar nucleus projects to the spiny output cells of the striatum matrix and the lateral amygdala, potentially relaying: (1) topographic visual information from SC to striatum to aid in guiding precise movements, and (2) non-topographic visual information from SC to the amygdala alerting the animal to potentially dangerous visual images.

No MeSH data available.


Related in: MedlinePlus

Pulvinar axons innervate the striatum matrix. (A) Confocal microscope image of substance P (SP, green) staining in the putamen. The matrix is sparsely innervated by thick SP axons, while the patches are densely innervated by fine SP axons. (B–D) Confocal image of a section stained for SP and parvalbumin (PV). The fine SP axons (purple, B) that innervate the patches interdigitate with a dense distribution of cells in the matrix that contain PV (C). (D) Merged image of (B) and (C) illustrates the interlocking arrangement of the SP-rich patches and the PV-rich matrix. (E–G) Pulvino-striatal axons innervate the PV-rich matrix. E illustrates BDA-labeled pulvino-striatal axons (purple) in the putamen. (F) shows a PV stained cell and dendrites (green) in the same section. (G) Merged image of (E) and (F) shows that pulvino-striatal axons target the PV-rich matrix. (H–J) Pulvino-striatal axons innervate the SP-poor matrix. (H) shows BDA-labeled pulvino-strital axons (purple) in the putamen. (I) shows SP staining (green) in the same section. Only the sparse SP axons of the matrix are stained. (J) A merged image of (H) and (I) illustrates that pulvino-striatal axons innervate the SP-poor matrix. All scale bars = 10 μm. Scale bar in (B) also applies to (C,D). Scale bar in (E) also applies to (F,G). Scale bar in (H) also applies to (I,J).
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Figure 4: Pulvinar axons innervate the striatum matrix. (A) Confocal microscope image of substance P (SP, green) staining in the putamen. The matrix is sparsely innervated by thick SP axons, while the patches are densely innervated by fine SP axons. (B–D) Confocal image of a section stained for SP and parvalbumin (PV). The fine SP axons (purple, B) that innervate the patches interdigitate with a dense distribution of cells in the matrix that contain PV (C). (D) Merged image of (B) and (C) illustrates the interlocking arrangement of the SP-rich patches and the PV-rich matrix. (E–G) Pulvino-striatal axons innervate the PV-rich matrix. E illustrates BDA-labeled pulvino-striatal axons (purple) in the putamen. (F) shows a PV stained cell and dendrites (green) in the same section. (G) Merged image of (E) and (F) shows that pulvino-striatal axons target the PV-rich matrix. (H–J) Pulvino-striatal axons innervate the SP-poor matrix. (H) shows BDA-labeled pulvino-strital axons (purple) in the putamen. (I) shows SP staining (green) in the same section. Only the sparse SP axons of the matrix are stained. (J) A merged image of (H) and (I) illustrates that pulvino-striatal axons innervate the SP-poor matrix. All scale bars = 10 μm. Scale bar in (B) also applies to (C,D). Scale bar in (E) also applies to (F,G). Scale bar in (H) also applies to (I,J).

Mentions: As illustrated in Figures 3 and 4, several different antibodies reveal the striosomes and matrix of the tree shrew caudate and putamen. An antibody against substance P stains dense patches of terminals (3A-C, G), while an antibody against parvalbumin intensely stained cells and neuropil within the matrix (3D, E). Interdigitating substance P and parvalbumin staining patterns are illustrated in Figures 4B–D. As recently demonstrated in primate tissue (Mikula et al., 2009), an antibody against KChIP stained cells throughout the matrix of the caudate and putamen and dense patches of terminals in striosomes (3F) in the tree shrew striatum.


Pulvinar projections to the striatum and amygdala in the tree shrew.

Day-Brown JD, Wei H, Chomsung RD, Petry HM, Bickford ME - Front Neuroanat (2010)

Pulvinar axons innervate the striatum matrix. (A) Confocal microscope image of substance P (SP, green) staining in the putamen. The matrix is sparsely innervated by thick SP axons, while the patches are densely innervated by fine SP axons. (B–D) Confocal image of a section stained for SP and parvalbumin (PV). The fine SP axons (purple, B) that innervate the patches interdigitate with a dense distribution of cells in the matrix that contain PV (C). (D) Merged image of (B) and (C) illustrates the interlocking arrangement of the SP-rich patches and the PV-rich matrix. (E–G) Pulvino-striatal axons innervate the PV-rich matrix. E illustrates BDA-labeled pulvino-striatal axons (purple) in the putamen. (F) shows a PV stained cell and dendrites (green) in the same section. (G) Merged image of (E) and (F) shows that pulvino-striatal axons target the PV-rich matrix. (H–J) Pulvino-striatal axons innervate the SP-poor matrix. (H) shows BDA-labeled pulvino-strital axons (purple) in the putamen. (I) shows SP staining (green) in the same section. Only the sparse SP axons of the matrix are stained. (J) A merged image of (H) and (I) illustrates that pulvino-striatal axons innervate the SP-poor matrix. All scale bars = 10 μm. Scale bar in (B) also applies to (C,D). Scale bar in (E) also applies to (F,G). Scale bar in (H) also applies to (I,J).
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Figure 4: Pulvinar axons innervate the striatum matrix. (A) Confocal microscope image of substance P (SP, green) staining in the putamen. The matrix is sparsely innervated by thick SP axons, while the patches are densely innervated by fine SP axons. (B–D) Confocal image of a section stained for SP and parvalbumin (PV). The fine SP axons (purple, B) that innervate the patches interdigitate with a dense distribution of cells in the matrix that contain PV (C). (D) Merged image of (B) and (C) illustrates the interlocking arrangement of the SP-rich patches and the PV-rich matrix. (E–G) Pulvino-striatal axons innervate the PV-rich matrix. E illustrates BDA-labeled pulvino-striatal axons (purple) in the putamen. (F) shows a PV stained cell and dendrites (green) in the same section. (G) Merged image of (E) and (F) shows that pulvino-striatal axons target the PV-rich matrix. (H–J) Pulvino-striatal axons innervate the SP-poor matrix. (H) shows BDA-labeled pulvino-strital axons (purple) in the putamen. (I) shows SP staining (green) in the same section. Only the sparse SP axons of the matrix are stained. (J) A merged image of (H) and (I) illustrates that pulvino-striatal axons innervate the SP-poor matrix. All scale bars = 10 μm. Scale bar in (B) also applies to (C,D). Scale bar in (E) also applies to (F,G). Scale bar in (H) also applies to (I,J).
Mentions: As illustrated in Figures 3 and 4, several different antibodies reveal the striosomes and matrix of the tree shrew caudate and putamen. An antibody against substance P stains dense patches of terminals (3A-C, G), while an antibody against parvalbumin intensely stained cells and neuropil within the matrix (3D, E). Interdigitating substance P and parvalbumin staining patterns are illustrated in Figures 4B–D. As recently demonstrated in primate tissue (Mikula et al., 2009), an antibody against KChIP stained cells throughout the matrix of the caudate and putamen and dense patches of terminals in striosomes (3F) in the tree shrew striatum.

Bottom Line: Both capabilities are thought to be mediated by pathways from the retina through the superior colliculus (SC) and pulvinar nucleus.Using immunocytochemical staining for substance P (SP) and parvalbumin (PV) to reveal the patch/matrix organization of tree shrew striatum, we found that SP-rich/PV-poor patches interlock with a PV-rich/SP-poor matrix.Electron microscopy revealed that the postsynaptic targets of tracer-labeled pulvino-striatal and pulvino-amygdala terminals are spines, demonstrating that the pulvinar nucleus projects to the spiny output cells of the striatum matrix and the lateral amygdala, potentially relaying: (1) topographic visual information from SC to striatum to aid in guiding precise movements, and (2) non-topographic visual information from SC to the amygdala alerting the animal to potentially dangerous visual images.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomical Sciences and Neurobiology, University of Louisville Medical Center Louisville, KY, USA.

ABSTRACT
Visually guided movement is possible in the absence of conscious visual perception, a phenomenon referred to as "blindsight." Similarly, fearful images can elicit emotional responses in the absence of their conscious perception. Both capabilities are thought to be mediated by pathways from the retina through the superior colliculus (SC) and pulvinar nucleus. To define potential pathways that underlie behavioral responses to unperceived visual stimuli, we examined the projections from the pulvinar nucleus to the striatum and amygdala in the tree shrew (Tupaia belangeri), a species considered to be a prototypical primate. The tree shrew brain has a large pulvinar nucleus that contains two SC-recipient subdivisions; the dorsal (Pd) and central (Pc) pulvinar both receive topographic ("specific") projections from SC, and Pd receives an additional non-topographic ("diffuse") projection from SC (Chomsung et al., 2008). Anterograde and retrograde tract tracing revealed that both Pd and Pc project to the caudate and putamen, and Pd, but not Pc, additionally projects to the lateral amygdala. Using immunocytochemical staining for substance P (SP) and parvalbumin (PV) to reveal the patch/matrix organization of tree shrew striatum, we found that SP-rich/PV-poor patches interlock with a PV-rich/SP-poor matrix. Confocal microscopy revealed that tracer-labeled pulvino-striatal terminals preferentially innervate the matrix. Electron microscopy revealed that the postsynaptic targets of tracer-labeled pulvino-striatal and pulvino-amygdala terminals are spines, demonstrating that the pulvinar nucleus projects to the spiny output cells of the striatum matrix and the lateral amygdala, potentially relaying: (1) topographic visual information from SC to striatum to aid in guiding precise movements, and (2) non-topographic visual information from SC to the amygdala alerting the animal to potentially dangerous visual images.

No MeSH data available.


Related in: MedlinePlus