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Pulvinar projections to the striatum and amygdala in the tree shrew.

Day-Brown JD, Wei H, Chomsung RD, Petry HM, Bickford ME - Front Neuroanat (2010)

Bottom Line: Both capabilities are thought to be mediated by pathways from the retina through the superior colliculus (SC) and pulvinar nucleus.Using immunocytochemical staining for substance P (SP) and parvalbumin (PV) to reveal the patch/matrix organization of tree shrew striatum, we found that SP-rich/PV-poor patches interlock with a PV-rich/SP-poor matrix.Electron microscopy revealed that the postsynaptic targets of tracer-labeled pulvino-striatal and pulvino-amygdala terminals are spines, demonstrating that the pulvinar nucleus projects to the spiny output cells of the striatum matrix and the lateral amygdala, potentially relaying: (1) topographic visual information from SC to striatum to aid in guiding precise movements, and (2) non-topographic visual information from SC to the amygdala alerting the animal to potentially dangerous visual images.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomical Sciences and Neurobiology, University of Louisville Medical Center Louisville, KY, USA.

ABSTRACT
Visually guided movement is possible in the absence of conscious visual perception, a phenomenon referred to as "blindsight." Similarly, fearful images can elicit emotional responses in the absence of their conscious perception. Both capabilities are thought to be mediated by pathways from the retina through the superior colliculus (SC) and pulvinar nucleus. To define potential pathways that underlie behavioral responses to unperceived visual stimuli, we examined the projections from the pulvinar nucleus to the striatum and amygdala in the tree shrew (Tupaia belangeri), a species considered to be a prototypical primate. The tree shrew brain has a large pulvinar nucleus that contains two SC-recipient subdivisions; the dorsal (Pd) and central (Pc) pulvinar both receive topographic ("specific") projections from SC, and Pd receives an additional non-topographic ("diffuse") projection from SC (Chomsung et al., 2008). Anterograde and retrograde tract tracing revealed that both Pd and Pc project to the caudate and putamen, and Pd, but not Pc, additionally projects to the lateral amygdala. Using immunocytochemical staining for substance P (SP) and parvalbumin (PV) to reveal the patch/matrix organization of tree shrew striatum, we found that SP-rich/PV-poor patches interlock with a PV-rich/SP-poor matrix. Confocal microscopy revealed that tracer-labeled pulvino-striatal terminals preferentially innervate the matrix. Electron microscopy revealed that the postsynaptic targets of tracer-labeled pulvino-striatal and pulvino-amygdala terminals are spines, demonstrating that the pulvinar nucleus projects to the spiny output cells of the striatum matrix and the lateral amygdala, potentially relaying: (1) topographic visual information from SC to striatum to aid in guiding precise movements, and (2) non-topographic visual information from SC to the amygdala alerting the animal to potentially dangerous visual images.

No MeSH data available.


Related in: MedlinePlus

Substance P (SP), parvalbumin (PV) and potassium channel interacting protein (KChIP) antibodies reveal patch/matrix organization of tree shrew striatum. (A,B) Sections (parasagittal, A; coronal, B) through the striatum stained for SP. Dark SP-positive patches are seen throughout the tree shrew striatum. (C,D) Adjacent sections through the putamen showing SP staining of patches (C) and PV staining in the matrix (D). (E) PV stains cells within the matrix but not within patches (black asterisks). (F) KChIP stains clusters of terminals in patches (white asterisk) and cells in the matrix. (G) SP stains densely distributed terminals in patches (white asterisk). In (A), scale bar = 1 mm and applies to B. Scale bar in C = 100 μm and applies to (D). Scale bar in E = 100 μm and applies to (F,G). Amg, amygdala, Cd, caudate, CL, claustrum, GP, globus pallidus, OT, optic tract, Put, putamen.
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Figure 3: Substance P (SP), parvalbumin (PV) and potassium channel interacting protein (KChIP) antibodies reveal patch/matrix organization of tree shrew striatum. (A,B) Sections (parasagittal, A; coronal, B) through the striatum stained for SP. Dark SP-positive patches are seen throughout the tree shrew striatum. (C,D) Adjacent sections through the putamen showing SP staining of patches (C) and PV staining in the matrix (D). (E) PV stains cells within the matrix but not within patches (black asterisks). (F) KChIP stains clusters of terminals in patches (white asterisk) and cells in the matrix. (G) SP stains densely distributed terminals in patches (white asterisk). In (A), scale bar = 1 mm and applies to B. Scale bar in C = 100 μm and applies to (D). Scale bar in E = 100 μm and applies to (F,G). Amg, amygdala, Cd, caudate, CL, claustrum, GP, globus pallidus, OT, optic tract, Put, putamen.

Mentions: As illustrated in Figures 3 and 4, several different antibodies reveal the striosomes and matrix of the tree shrew caudate and putamen. An antibody against substance P stains dense patches of terminals (3A-C, G), while an antibody against parvalbumin intensely stained cells and neuropil within the matrix (3D, E). Interdigitating substance P and parvalbumin staining patterns are illustrated in Figures 4B–D. As recently demonstrated in primate tissue (Mikula et al., 2009), an antibody against KChIP stained cells throughout the matrix of the caudate and putamen and dense patches of terminals in striosomes (3F) in the tree shrew striatum.


Pulvinar projections to the striatum and amygdala in the tree shrew.

Day-Brown JD, Wei H, Chomsung RD, Petry HM, Bickford ME - Front Neuroanat (2010)

Substance P (SP), parvalbumin (PV) and potassium channel interacting protein (KChIP) antibodies reveal patch/matrix organization of tree shrew striatum. (A,B) Sections (parasagittal, A; coronal, B) through the striatum stained for SP. Dark SP-positive patches are seen throughout the tree shrew striatum. (C,D) Adjacent sections through the putamen showing SP staining of patches (C) and PV staining in the matrix (D). (E) PV stains cells within the matrix but not within patches (black asterisks). (F) KChIP stains clusters of terminals in patches (white asterisk) and cells in the matrix. (G) SP stains densely distributed terminals in patches (white asterisk). In (A), scale bar = 1 mm and applies to B. Scale bar in C = 100 μm and applies to (D). Scale bar in E = 100 μm and applies to (F,G). Amg, amygdala, Cd, caudate, CL, claustrum, GP, globus pallidus, OT, optic tract, Put, putamen.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC2991220&req=5

Figure 3: Substance P (SP), parvalbumin (PV) and potassium channel interacting protein (KChIP) antibodies reveal patch/matrix organization of tree shrew striatum. (A,B) Sections (parasagittal, A; coronal, B) through the striatum stained for SP. Dark SP-positive patches are seen throughout the tree shrew striatum. (C,D) Adjacent sections through the putamen showing SP staining of patches (C) and PV staining in the matrix (D). (E) PV stains cells within the matrix but not within patches (black asterisks). (F) KChIP stains clusters of terminals in patches (white asterisk) and cells in the matrix. (G) SP stains densely distributed terminals in patches (white asterisk). In (A), scale bar = 1 mm and applies to B. Scale bar in C = 100 μm and applies to (D). Scale bar in E = 100 μm and applies to (F,G). Amg, amygdala, Cd, caudate, CL, claustrum, GP, globus pallidus, OT, optic tract, Put, putamen.
Mentions: As illustrated in Figures 3 and 4, several different antibodies reveal the striosomes and matrix of the tree shrew caudate and putamen. An antibody against substance P stains dense patches of terminals (3A-C, G), while an antibody against parvalbumin intensely stained cells and neuropil within the matrix (3D, E). Interdigitating substance P and parvalbumin staining patterns are illustrated in Figures 4B–D. As recently demonstrated in primate tissue (Mikula et al., 2009), an antibody against KChIP stained cells throughout the matrix of the caudate and putamen and dense patches of terminals in striosomes (3F) in the tree shrew striatum.

Bottom Line: Both capabilities are thought to be mediated by pathways from the retina through the superior colliculus (SC) and pulvinar nucleus.Using immunocytochemical staining for substance P (SP) and parvalbumin (PV) to reveal the patch/matrix organization of tree shrew striatum, we found that SP-rich/PV-poor patches interlock with a PV-rich/SP-poor matrix.Electron microscopy revealed that the postsynaptic targets of tracer-labeled pulvino-striatal and pulvino-amygdala terminals are spines, demonstrating that the pulvinar nucleus projects to the spiny output cells of the striatum matrix and the lateral amygdala, potentially relaying: (1) topographic visual information from SC to striatum to aid in guiding precise movements, and (2) non-topographic visual information from SC to the amygdala alerting the animal to potentially dangerous visual images.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomical Sciences and Neurobiology, University of Louisville Medical Center Louisville, KY, USA.

ABSTRACT
Visually guided movement is possible in the absence of conscious visual perception, a phenomenon referred to as "blindsight." Similarly, fearful images can elicit emotional responses in the absence of their conscious perception. Both capabilities are thought to be mediated by pathways from the retina through the superior colliculus (SC) and pulvinar nucleus. To define potential pathways that underlie behavioral responses to unperceived visual stimuli, we examined the projections from the pulvinar nucleus to the striatum and amygdala in the tree shrew (Tupaia belangeri), a species considered to be a prototypical primate. The tree shrew brain has a large pulvinar nucleus that contains two SC-recipient subdivisions; the dorsal (Pd) and central (Pc) pulvinar both receive topographic ("specific") projections from SC, and Pd receives an additional non-topographic ("diffuse") projection from SC (Chomsung et al., 2008). Anterograde and retrograde tract tracing revealed that both Pd and Pc project to the caudate and putamen, and Pd, but not Pc, additionally projects to the lateral amygdala. Using immunocytochemical staining for substance P (SP) and parvalbumin (PV) to reveal the patch/matrix organization of tree shrew striatum, we found that SP-rich/PV-poor patches interlock with a PV-rich/SP-poor matrix. Confocal microscopy revealed that tracer-labeled pulvino-striatal terminals preferentially innervate the matrix. Electron microscopy revealed that the postsynaptic targets of tracer-labeled pulvino-striatal and pulvino-amygdala terminals are spines, demonstrating that the pulvinar nucleus projects to the spiny output cells of the striatum matrix and the lateral amygdala, potentially relaying: (1) topographic visual information from SC to striatum to aid in guiding precise movements, and (2) non-topographic visual information from SC to the amygdala alerting the animal to potentially dangerous visual images.

No MeSH data available.


Related in: MedlinePlus