Limits...
Pulvinar projections to the striatum and amygdala in the tree shrew.

Day-Brown JD, Wei H, Chomsung RD, Petry HM, Bickford ME - Front Neuroanat (2010)

Bottom Line: Both capabilities are thought to be mediated by pathways from the retina through the superior colliculus (SC) and pulvinar nucleus.Using immunocytochemical staining for substance P (SP) and parvalbumin (PV) to reveal the patch/matrix organization of tree shrew striatum, we found that SP-rich/PV-poor patches interlock with a PV-rich/SP-poor matrix.Electron microscopy revealed that the postsynaptic targets of tracer-labeled pulvino-striatal and pulvino-amygdala terminals are spines, demonstrating that the pulvinar nucleus projects to the spiny output cells of the striatum matrix and the lateral amygdala, potentially relaying: (1) topographic visual information from SC to striatum to aid in guiding precise movements, and (2) non-topographic visual information from SC to the amygdala alerting the animal to potentially dangerous visual images.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomical Sciences and Neurobiology, University of Louisville Medical Center Louisville, KY, USA.

ABSTRACT
Visually guided movement is possible in the absence of conscious visual perception, a phenomenon referred to as "blindsight." Similarly, fearful images can elicit emotional responses in the absence of their conscious perception. Both capabilities are thought to be mediated by pathways from the retina through the superior colliculus (SC) and pulvinar nucleus. To define potential pathways that underlie behavioral responses to unperceived visual stimuli, we examined the projections from the pulvinar nucleus to the striatum and amygdala in the tree shrew (Tupaia belangeri), a species considered to be a prototypical primate. The tree shrew brain has a large pulvinar nucleus that contains two SC-recipient subdivisions; the dorsal (Pd) and central (Pc) pulvinar both receive topographic ("specific") projections from SC, and Pd receives an additional non-topographic ("diffuse") projection from SC (Chomsung et al., 2008). Anterograde and retrograde tract tracing revealed that both Pd and Pc project to the caudate and putamen, and Pd, but not Pc, additionally projects to the lateral amygdala. Using immunocytochemical staining for substance P (SP) and parvalbumin (PV) to reveal the patch/matrix organization of tree shrew striatum, we found that SP-rich/PV-poor patches interlock with a PV-rich/SP-poor matrix. Confocal microscopy revealed that tracer-labeled pulvino-striatal terminals preferentially innervate the matrix. Electron microscopy revealed that the postsynaptic targets of tracer-labeled pulvino-striatal and pulvino-amygdala terminals are spines, demonstrating that the pulvinar nucleus projects to the spiny output cells of the striatum matrix and the lateral amygdala, potentially relaying: (1) topographic visual information from SC to striatum to aid in guiding precise movements, and (2) non-topographic visual information from SC to the amygdala alerting the animal to potentially dangerous visual images.

No MeSH data available.


Related in: MedlinePlus

The dorsal (Pd) and central (Pc) pulvinar project to the striatum; only the Pd projects to the amygdala. (A–C) Two dimensional (2D) neurolucida plots of the distribution of terminals labeled by anterograde transport in the caudate (Cd) and putamen (PUT) following three separate injections of BDA in the Pc. (D) Injection sites for (A–C) depicted in three-dimensional (3D) neurolucida reconstructions of the pulvinar nucleus. The color of each injection site corresponds to the color of the terminals in (A–C). (E–G) Two-dimensional plots of the distribution of terminals labeled by anterograde transport in the PUT and amygdala (AMG) following three separate injections of BDA in the Pd. (H) Three-dimension reconstructions of injection sites for (E–G). Because the full extent of the Pd is not viewable in the orientation depicted, the Pc subdivision was made transparent in this panel as well as in (J,K). (I) Two-dimension plots of the location of FG and CTB injections in the PUT and AMG. (J,K,L) Three-dimension reconstructions of the distribution of cells labeled in the pulvinar nucleus by retrograde transport from the injections illustrated in I. The color of the cell distributions corresponds to the injection site colors in I. Scale bar in A = 1 mm and applies to all 2D plots. Scale bar in D = 1 mm and applies to all 3D pulvinar reconstructions. C, caudal, CL, claustrum, D, dorsal, OC, optic chiasm, OT, optic tract, R, rostral, V, ventral. Orientation arrows in D also apply to all 3D pulvinar reconstructions.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2991220&req=5

Figure 2: The dorsal (Pd) and central (Pc) pulvinar project to the striatum; only the Pd projects to the amygdala. (A–C) Two dimensional (2D) neurolucida plots of the distribution of terminals labeled by anterograde transport in the caudate (Cd) and putamen (PUT) following three separate injections of BDA in the Pc. (D) Injection sites for (A–C) depicted in three-dimensional (3D) neurolucida reconstructions of the pulvinar nucleus. The color of each injection site corresponds to the color of the terminals in (A–C). (E–G) Two-dimensional plots of the distribution of terminals labeled by anterograde transport in the PUT and amygdala (AMG) following three separate injections of BDA in the Pd. (H) Three-dimension reconstructions of injection sites for (E–G). Because the full extent of the Pd is not viewable in the orientation depicted, the Pc subdivision was made transparent in this panel as well as in (J,K). (I) Two-dimension plots of the location of FG and CTB injections in the PUT and AMG. (J,K,L) Three-dimension reconstructions of the distribution of cells labeled in the pulvinar nucleus by retrograde transport from the injections illustrated in I. The color of the cell distributions corresponds to the injection site colors in I. Scale bar in A = 1 mm and applies to all 2D plots. Scale bar in D = 1 mm and applies to all 3D pulvinar reconstructions. C, caudal, CL, claustrum, D, dorsal, OC, optic chiasm, OT, optic tract, R, rostral, V, ventral. Orientation arrows in D also apply to all 3D pulvinar reconstructions.

Mentions: Following large BDA injections that involved both the Pd and Pc (Figure 1A), terminals labeled by anterograde transport fill large regions of the postcommisural putamen (Figure 1B) caudate (Figure 1C), as well as the lateral amygdala (Figure 1F). No pulvino-striatal terminals were distributed in regions of the caudate and putamen rostral to the anterior commissure. Smaller injections, confined to either the Pd or Pc (example Pc injection illustrated in Figure 1D; all other injection sites illustrated in Chomsung et al., 2008, 2010), labeled more discrete clusters of terminals in the caudate (Figures 2A–C) and putamen (Figures 1E, 2A–C, E–G), and injections of the Pd, but not the Pc, also labeled diffusely distributed axons in the lateral amygdala (Figures 2E–G).


Pulvinar projections to the striatum and amygdala in the tree shrew.

Day-Brown JD, Wei H, Chomsung RD, Petry HM, Bickford ME - Front Neuroanat (2010)

The dorsal (Pd) and central (Pc) pulvinar project to the striatum; only the Pd projects to the amygdala. (A–C) Two dimensional (2D) neurolucida plots of the distribution of terminals labeled by anterograde transport in the caudate (Cd) and putamen (PUT) following three separate injections of BDA in the Pc. (D) Injection sites for (A–C) depicted in three-dimensional (3D) neurolucida reconstructions of the pulvinar nucleus. The color of each injection site corresponds to the color of the terminals in (A–C). (E–G) Two-dimensional plots of the distribution of terminals labeled by anterograde transport in the PUT and amygdala (AMG) following three separate injections of BDA in the Pd. (H) Three-dimension reconstructions of injection sites for (E–G). Because the full extent of the Pd is not viewable in the orientation depicted, the Pc subdivision was made transparent in this panel as well as in (J,K). (I) Two-dimension plots of the location of FG and CTB injections in the PUT and AMG. (J,K,L) Three-dimension reconstructions of the distribution of cells labeled in the pulvinar nucleus by retrograde transport from the injections illustrated in I. The color of the cell distributions corresponds to the injection site colors in I. Scale bar in A = 1 mm and applies to all 2D plots. Scale bar in D = 1 mm and applies to all 3D pulvinar reconstructions. C, caudal, CL, claustrum, D, dorsal, OC, optic chiasm, OT, optic tract, R, rostral, V, ventral. Orientation arrows in D also apply to all 3D pulvinar reconstructions.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2991220&req=5

Figure 2: The dorsal (Pd) and central (Pc) pulvinar project to the striatum; only the Pd projects to the amygdala. (A–C) Two dimensional (2D) neurolucida plots of the distribution of terminals labeled by anterograde transport in the caudate (Cd) and putamen (PUT) following three separate injections of BDA in the Pc. (D) Injection sites for (A–C) depicted in three-dimensional (3D) neurolucida reconstructions of the pulvinar nucleus. The color of each injection site corresponds to the color of the terminals in (A–C). (E–G) Two-dimensional plots of the distribution of terminals labeled by anterograde transport in the PUT and amygdala (AMG) following three separate injections of BDA in the Pd. (H) Three-dimension reconstructions of injection sites for (E–G). Because the full extent of the Pd is not viewable in the orientation depicted, the Pc subdivision was made transparent in this panel as well as in (J,K). (I) Two-dimension plots of the location of FG and CTB injections in the PUT and AMG. (J,K,L) Three-dimension reconstructions of the distribution of cells labeled in the pulvinar nucleus by retrograde transport from the injections illustrated in I. The color of the cell distributions corresponds to the injection site colors in I. Scale bar in A = 1 mm and applies to all 2D plots. Scale bar in D = 1 mm and applies to all 3D pulvinar reconstructions. C, caudal, CL, claustrum, D, dorsal, OC, optic chiasm, OT, optic tract, R, rostral, V, ventral. Orientation arrows in D also apply to all 3D pulvinar reconstructions.
Mentions: Following large BDA injections that involved both the Pd and Pc (Figure 1A), terminals labeled by anterograde transport fill large regions of the postcommisural putamen (Figure 1B) caudate (Figure 1C), as well as the lateral amygdala (Figure 1F). No pulvino-striatal terminals were distributed in regions of the caudate and putamen rostral to the anterior commissure. Smaller injections, confined to either the Pd or Pc (example Pc injection illustrated in Figure 1D; all other injection sites illustrated in Chomsung et al., 2008, 2010), labeled more discrete clusters of terminals in the caudate (Figures 2A–C) and putamen (Figures 1E, 2A–C, E–G), and injections of the Pd, but not the Pc, also labeled diffusely distributed axons in the lateral amygdala (Figures 2E–G).

Bottom Line: Both capabilities are thought to be mediated by pathways from the retina through the superior colliculus (SC) and pulvinar nucleus.Using immunocytochemical staining for substance P (SP) and parvalbumin (PV) to reveal the patch/matrix organization of tree shrew striatum, we found that SP-rich/PV-poor patches interlock with a PV-rich/SP-poor matrix.Electron microscopy revealed that the postsynaptic targets of tracer-labeled pulvino-striatal and pulvino-amygdala terminals are spines, demonstrating that the pulvinar nucleus projects to the spiny output cells of the striatum matrix and the lateral amygdala, potentially relaying: (1) topographic visual information from SC to striatum to aid in guiding precise movements, and (2) non-topographic visual information from SC to the amygdala alerting the animal to potentially dangerous visual images.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomical Sciences and Neurobiology, University of Louisville Medical Center Louisville, KY, USA.

ABSTRACT
Visually guided movement is possible in the absence of conscious visual perception, a phenomenon referred to as "blindsight." Similarly, fearful images can elicit emotional responses in the absence of their conscious perception. Both capabilities are thought to be mediated by pathways from the retina through the superior colliculus (SC) and pulvinar nucleus. To define potential pathways that underlie behavioral responses to unperceived visual stimuli, we examined the projections from the pulvinar nucleus to the striatum and amygdala in the tree shrew (Tupaia belangeri), a species considered to be a prototypical primate. The tree shrew brain has a large pulvinar nucleus that contains two SC-recipient subdivisions; the dorsal (Pd) and central (Pc) pulvinar both receive topographic ("specific") projections from SC, and Pd receives an additional non-topographic ("diffuse") projection from SC (Chomsung et al., 2008). Anterograde and retrograde tract tracing revealed that both Pd and Pc project to the caudate and putamen, and Pd, but not Pc, additionally projects to the lateral amygdala. Using immunocytochemical staining for substance P (SP) and parvalbumin (PV) to reveal the patch/matrix organization of tree shrew striatum, we found that SP-rich/PV-poor patches interlock with a PV-rich/SP-poor matrix. Confocal microscopy revealed that tracer-labeled pulvino-striatal terminals preferentially innervate the matrix. Electron microscopy revealed that the postsynaptic targets of tracer-labeled pulvino-striatal and pulvino-amygdala terminals are spines, demonstrating that the pulvinar nucleus projects to the spiny output cells of the striatum matrix and the lateral amygdala, potentially relaying: (1) topographic visual information from SC to striatum to aid in guiding precise movements, and (2) non-topographic visual information from SC to the amygdala alerting the animal to potentially dangerous visual images.

No MeSH data available.


Related in: MedlinePlus