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Correlated Levels of mRNA and Soma Size in Single Identified Neurons: Evidence for Compartment-specific Regulation of Gene Expression.

Ransdell JL, Faust TB, Schulz DJ - Front Mol Neurosci (2010)

Bottom Line: Levels of mRNA for the K+ channel shal, which is localized exclusively to the soma, are negatively correlated with soma size, suggesting that gene expression does not simply track positively with compartment size.Conversely, levels of beta-actin and beta-tubulin mRNA, which are major cytoskeletal proteins of neuronal processes, do not correlate with soma size, but are strongly correlated with one another.These results suggest that steady-state transcript levels are differentially regulated based on the subcellular compartment within which a given gene product primarily acts.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, University of Missouri Columbia Columbia, MO, USA.

ABSTRACT
In addition to the overall complexity of transcriptional regulation, cells also must take into account the subcellular distribution of these gene products. This is particularly challenging for morphologically complex cells such as neurons. Yet the interaction between cellular morphology and gene expression is poorly understood. Here we provide some of the first evidence for a relationship between neuronal compartment size and maintenance of mRNA levels in neurons. We find that single-cell transcript levels of 18S rRNA, GAPDH, and EF1-alpha, all gene products with primary functions in the cell soma, are strongly correlated to soma size in multiple distinct neuronal types. Levels of mRNA for the K+ channel shal, which is localized exclusively to the soma, are negatively correlated with soma size, suggesting that gene expression does not simply track positively with compartment size. Conversely, levels of beta-actin and beta-tubulin mRNA, which are major cytoskeletal proteins of neuronal processes, do not correlate with soma size, but are strongly correlated with one another. Additionally, actin/tubulin expression levels correlate with voltage-gated ion channels that are uniquely localized to axons. These results suggest that steady-state transcript levels are differentially regulated based on the subcellular compartment within which a given gene product primarily acts.

No MeSH data available.


Related in: MedlinePlus

Correlations between levels of gene expression (y-axes) and cell soma size (x-axes) for five different genes (A – E). Points represent mean ± SEM. Individual neuron types as labeled, sample sizes listed in figure 1. Relative measurements are made with PD neurons as the reference population. R2 values are results of Pearson's correlation analyses.
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Figure 3: Correlations between levels of gene expression (y-axes) and cell soma size (x-axes) for five different genes (A – E). Points represent mean ± SEM. Individual neuron types as labeled, sample sizes listed in figure 1. Relative measurements are made with PD neurons as the reference population. R2 values are results of Pearson's correlation analyses.

Mentions: To more thoroughly examine the relationships among gene expression and cell soma size, we performed correlation analyses. 18S rRNA expression and cell soma size were strongly correlated (p < 0.001; R2 = 0.98; Figure 3A). EF1-alpha (p < 0.005; R2 = 0.91; Figure 3B) and GAPDH (p < 0.005; R2 = 0.91; Figure 3C) also were significantly correlated with cell soma size. Conversely, there were only weak and not statistically significant relationships between cell soma size and expression of actin (p = 0.08; R2 = 0.57; Figure 3D), and tubulin (p = 0.06; R2 = 0.62; Figure 3E).


Correlated Levels of mRNA and Soma Size in Single Identified Neurons: Evidence for Compartment-specific Regulation of Gene Expression.

Ransdell JL, Faust TB, Schulz DJ - Front Mol Neurosci (2010)

Correlations between levels of gene expression (y-axes) and cell soma size (x-axes) for five different genes (A – E). Points represent mean ± SEM. Individual neuron types as labeled, sample sizes listed in figure 1. Relative measurements are made with PD neurons as the reference population. R2 values are results of Pearson's correlation analyses.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2991126&req=5

Figure 3: Correlations between levels of gene expression (y-axes) and cell soma size (x-axes) for five different genes (A – E). Points represent mean ± SEM. Individual neuron types as labeled, sample sizes listed in figure 1. Relative measurements are made with PD neurons as the reference population. R2 values are results of Pearson's correlation analyses.
Mentions: To more thoroughly examine the relationships among gene expression and cell soma size, we performed correlation analyses. 18S rRNA expression and cell soma size were strongly correlated (p < 0.001; R2 = 0.98; Figure 3A). EF1-alpha (p < 0.005; R2 = 0.91; Figure 3B) and GAPDH (p < 0.005; R2 = 0.91; Figure 3C) also were significantly correlated with cell soma size. Conversely, there were only weak and not statistically significant relationships between cell soma size and expression of actin (p = 0.08; R2 = 0.57; Figure 3D), and tubulin (p = 0.06; R2 = 0.62; Figure 3E).

Bottom Line: Levels of mRNA for the K+ channel shal, which is localized exclusively to the soma, are negatively correlated with soma size, suggesting that gene expression does not simply track positively with compartment size.Conversely, levels of beta-actin and beta-tubulin mRNA, which are major cytoskeletal proteins of neuronal processes, do not correlate with soma size, but are strongly correlated with one another.These results suggest that steady-state transcript levels are differentially regulated based on the subcellular compartment within which a given gene product primarily acts.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, University of Missouri Columbia Columbia, MO, USA.

ABSTRACT
In addition to the overall complexity of transcriptional regulation, cells also must take into account the subcellular distribution of these gene products. This is particularly challenging for morphologically complex cells such as neurons. Yet the interaction between cellular morphology and gene expression is poorly understood. Here we provide some of the first evidence for a relationship between neuronal compartment size and maintenance of mRNA levels in neurons. We find that single-cell transcript levels of 18S rRNA, GAPDH, and EF1-alpha, all gene products with primary functions in the cell soma, are strongly correlated to soma size in multiple distinct neuronal types. Levels of mRNA for the K+ channel shal, which is localized exclusively to the soma, are negatively correlated with soma size, suggesting that gene expression does not simply track positively with compartment size. Conversely, levels of beta-actin and beta-tubulin mRNA, which are major cytoskeletal proteins of neuronal processes, do not correlate with soma size, but are strongly correlated with one another. Additionally, actin/tubulin expression levels correlate with voltage-gated ion channels that are uniquely localized to axons. These results suggest that steady-state transcript levels are differentially regulated based on the subcellular compartment within which a given gene product primarily acts.

No MeSH data available.


Related in: MedlinePlus