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Appetitive operant conditioning in mice: heritability and dissociability of training stages.

Malkki HA, Donga LA, de Groot SE, Battaglia FP, NeuroBSIK Mouse Phenomics ConsortiumPennartz CM - Front Behav Neurosci (2010)

Bottom Line: We also computed correlations between successive training stages to study whether learning deficits at an advanced stage of operant conditioning may be dissociated from normal performance in preceding phases of training.Quantitative trait loci mapping revealed suggestive likelihood ratio statistic peaks for initial magazine checking behavior and lever press-NP.These heritable components may reside in different chromosomal areas.

View Article: PubMed Central - PubMed

Affiliation: Cognitive and Systems Neuroscience, Center for Neuroscience, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, Netherlands. h.a.i.malkki@uva.nl

ABSTRACT
To study the heritability of different training stages of appetitive operant conditioning, we carried out behavioral screening of 5 standard inbred mouse strains, 28 recombinant-inbred (BxD) mouse lines and their progenitor strains C57BL/6J and DBA/2J. We also computed correlations between successive training stages to study whether learning deficits at an advanced stage of operant conditioning may be dissociated from normal performance in preceding phases of training. The training consisted of two phases: an operant nose poking (NP) phase, in which mice learned to collect a sucrose pellet from a food magazine by NP, and an operant lever press and NP phase, in which mice had to execute a sequence of these two actions to collect a food pellet. As a measure of magazine oriented exploration, we also studied the nose poke entries in the food magazine during the intertrial intervals at the beginning of the first session of the nose poke training phase. We found significantly heritable components in initial magazine checking behavior, operant NP and lever press-NP. Performance levels in these phases were positively correlated, but several individual strains were identified that showed poor lever press-NP while performing well in preceding training stages. Quantitative trait loci mapping revealed suggestive likelihood ratio statistic peaks for initial magazine checking behavior and lever press-NP. These findings indicate that consecutive stages toward more complex operant behavior show significant heritable components, as well as dissociability between stages in specific mouse strains. These heritable components may reside in different chromosomal areas.

No MeSH data available.


Related in: MedlinePlus

Acquisition of lever press–nose poke performance Lever press–nose poke performance percentages over the course of five training sessions for each strain. Strain means presented in color-coding, see color bar on the right; color scale ranges from 0 to 100% correct performance. Strains are sorted based on their level of performance. A few of the mice did not complete the 5th session of training (which causes the drop in performance of lines 87 and 1), in which case we used the average of the last session of each mouse as the best available approximation for the final performance of the strain (Figure 2C).
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Figure 3: Acquisition of lever press–nose poke performance Lever press–nose poke performance percentages over the course of five training sessions for each strain. Strain means presented in color-coding, see color bar on the right; color scale ranges from 0 to 100% correct performance. Strains are sorted based on their level of performance. A few of the mice did not complete the 5th session of training (which causes the drop in performance of lines 87 and 1), in which case we used the average of the last session of each mouse as the best available approximation for the final performance of the strain (Figure 2C).

Mentions: Over the course of training on this task, one line (BxD-90) failed to complete any trials despite showing clear nose poke behavior in the earlier training phase (Figure 2C). Most strains showed improving performance over the five training sessions (Figure 3). In the last, fifth session of training, performance in the operant task varied remarkably across strains: performance varied from 0.0% (BxD-90) to 99.6% (NOD/Ltj), the average being 46.4 ± 4.9% (Figure 2C).


Appetitive operant conditioning in mice: heritability and dissociability of training stages.

Malkki HA, Donga LA, de Groot SE, Battaglia FP, NeuroBSIK Mouse Phenomics ConsortiumPennartz CM - Front Behav Neurosci (2010)

Acquisition of lever press–nose poke performance Lever press–nose poke performance percentages over the course of five training sessions for each strain. Strain means presented in color-coding, see color bar on the right; color scale ranges from 0 to 100% correct performance. Strains are sorted based on their level of performance. A few of the mice did not complete the 5th session of training (which causes the drop in performance of lines 87 and 1), in which case we used the average of the last session of each mouse as the best available approximation for the final performance of the strain (Figure 2C).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2990458&req=5

Figure 3: Acquisition of lever press–nose poke performance Lever press–nose poke performance percentages over the course of five training sessions for each strain. Strain means presented in color-coding, see color bar on the right; color scale ranges from 0 to 100% correct performance. Strains are sorted based on their level of performance. A few of the mice did not complete the 5th session of training (which causes the drop in performance of lines 87 and 1), in which case we used the average of the last session of each mouse as the best available approximation for the final performance of the strain (Figure 2C).
Mentions: Over the course of training on this task, one line (BxD-90) failed to complete any trials despite showing clear nose poke behavior in the earlier training phase (Figure 2C). Most strains showed improving performance over the five training sessions (Figure 3). In the last, fifth session of training, performance in the operant task varied remarkably across strains: performance varied from 0.0% (BxD-90) to 99.6% (NOD/Ltj), the average being 46.4 ± 4.9% (Figure 2C).

Bottom Line: We also computed correlations between successive training stages to study whether learning deficits at an advanced stage of operant conditioning may be dissociated from normal performance in preceding phases of training.Quantitative trait loci mapping revealed suggestive likelihood ratio statistic peaks for initial magazine checking behavior and lever press-NP.These heritable components may reside in different chromosomal areas.

View Article: PubMed Central - PubMed

Affiliation: Cognitive and Systems Neuroscience, Center for Neuroscience, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, Netherlands. h.a.i.malkki@uva.nl

ABSTRACT
To study the heritability of different training stages of appetitive operant conditioning, we carried out behavioral screening of 5 standard inbred mouse strains, 28 recombinant-inbred (BxD) mouse lines and their progenitor strains C57BL/6J and DBA/2J. We also computed correlations between successive training stages to study whether learning deficits at an advanced stage of operant conditioning may be dissociated from normal performance in preceding phases of training. The training consisted of two phases: an operant nose poking (NP) phase, in which mice learned to collect a sucrose pellet from a food magazine by NP, and an operant lever press and NP phase, in which mice had to execute a sequence of these two actions to collect a food pellet. As a measure of magazine oriented exploration, we also studied the nose poke entries in the food magazine during the intertrial intervals at the beginning of the first session of the nose poke training phase. We found significantly heritable components in initial magazine checking behavior, operant NP and lever press-NP. Performance levels in these phases were positively correlated, but several individual strains were identified that showed poor lever press-NP while performing well in preceding training stages. Quantitative trait loci mapping revealed suggestive likelihood ratio statistic peaks for initial magazine checking behavior and lever press-NP. These findings indicate that consecutive stages toward more complex operant behavior show significant heritable components, as well as dissociability between stages in specific mouse strains. These heritable components may reside in different chromosomal areas.

No MeSH data available.


Related in: MedlinePlus