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A new nucleolar body appears in Drosophila saltans salivary gland cells before histolysis, in programmed cell death.

de Oliveira CC, de Campos Bicudo HE - J. Insect Sci. (2006)

Bottom Line: In many cells, the new nucleolar corpuscle and these chromosome threads are associated.These findings are novel.However, the hypothesis put forward concerning their meaning remains dependent on other studies.

View Article: PubMed Central - PubMed

Affiliation: Program in Ecology and Evolutionary Biology, Department of Biological Sciences, University of Arkansas, Fayetteville, AR 72701, USA. cdeolive@uark.edu

ABSTRACT
The salivary glands of Drosophila saltans (saltans group, saltans subgroup) analyzed in an advanced stage of programmed cell death showed the appearance of a single, round, nucleolar corpuscle inside the highly altered nucleus of every gland cell, at a time during which the integrity of the original nucleolus was already lost and the original nucleolar material apparently disappeared. In the same nuclei, which already had also lost the characteristic chromosome structure, some delicate chromosome threads were maintained. In many cells, the new nucleolar corpuscle and these chromosome threads are associated. These findings are novel. However, the hypothesis put forward concerning their meaning remains dependent on other studies.

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A-H. Salivary gland cells in different stages of programmed cell death. A. Orange-stained nucleolus (Nu) and green-stained chromosomes in a third instar larva cell stained with acridine orange technique. B. Advanced stage of chromosome alteration; the chromosomes remain green with acridine orange technique. C, D. Cells in very advanced stage of programmed cell death: the new nucleolar body (arrows) is associated with green-stained remains of chromosome material in two disrupted cells (C), or associated with the nuclear membrane (D). E-H. Cells also in very advanced stage of programmed cell death, stained with acridine orange (E, G) and with Ag-Nor (F, H). The arrows point to the same nucleolar bodies in slides stained with the two techniques. In H, the highlighted nucleolus is associated with fine, silver stained threads. In C to H, the cytoplasmic material looks very dense. Scale bars: A = 5.0 µm; B, C, D = 7.2 µm; E-H = 11.5 µm.
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f02: A-H. Salivary gland cells in different stages of programmed cell death. A. Orange-stained nucleolus (Nu) and green-stained chromosomes in a third instar larva cell stained with acridine orange technique. B. Advanced stage of chromosome alteration; the chromosomes remain green with acridine orange technique. C, D. Cells in very advanced stage of programmed cell death: the new nucleolar body (arrows) is associated with green-stained remains of chromosome material in two disrupted cells (C), or associated with the nuclear membrane (D). E-H. Cells also in very advanced stage of programmed cell death, stained with acridine orange (E, G) and with Ag-Nor (F, H). The arrows point to the same nucleolar bodies in slides stained with the two techniques. In H, the highlighted nucleolus is associated with fine, silver stained threads. In C to H, the cytoplasmic material looks very dense. Scale bars: A = 5.0 µm; B, C, D = 7.2 µm; E-H = 11.5 µm.

Mentions: In acridine orange stained slides of late third instar larvae, the chromosomes were green and the nucleolus was orange or red, as expected on the basis of the properties of the stain. The chromatin associated with the nucleolus was well marked, as well as the nucleolus connection with the chromocenter region (Figure 2A). As development progresses, gradual loss of the chromosome-banding pattern, loss of the chromosome individuality, and transformation of the chromosomes into tenuous masses of filaments or tangled threads were observed (Figures 1F, 2B). The nucleolar integrity was also lost in parallel with the loss of its characteristic pattern of silver nitrate impregnation that either became restricted to inner regions of the nucleolus or became irregular (Figure1D, E), unlike the larval stage in which impregnation reaches the whole nucleolar mass. The nucleolar alterations increased until a differentiated nucleolar structure is no longer visible.


A new nucleolar body appears in Drosophila saltans salivary gland cells before histolysis, in programmed cell death.

de Oliveira CC, de Campos Bicudo HE - J. Insect Sci. (2006)

A-H. Salivary gland cells in different stages of programmed cell death. A. Orange-stained nucleolus (Nu) and green-stained chromosomes in a third instar larva cell stained with acridine orange technique. B. Advanced stage of chromosome alteration; the chromosomes remain green with acridine orange technique. C, D. Cells in very advanced stage of programmed cell death: the new nucleolar body (arrows) is associated with green-stained remains of chromosome material in two disrupted cells (C), or associated with the nuclear membrane (D). E-H. Cells also in very advanced stage of programmed cell death, stained with acridine orange (E, G) and with Ag-Nor (F, H). The arrows point to the same nucleolar bodies in slides stained with the two techniques. In H, the highlighted nucleolus is associated with fine, silver stained threads. In C to H, the cytoplasmic material looks very dense. Scale bars: A = 5.0 µm; B, C, D = 7.2 µm; E-H = 11.5 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2990330&req=5

f02: A-H. Salivary gland cells in different stages of programmed cell death. A. Orange-stained nucleolus (Nu) and green-stained chromosomes in a third instar larva cell stained with acridine orange technique. B. Advanced stage of chromosome alteration; the chromosomes remain green with acridine orange technique. C, D. Cells in very advanced stage of programmed cell death: the new nucleolar body (arrows) is associated with green-stained remains of chromosome material in two disrupted cells (C), or associated with the nuclear membrane (D). E-H. Cells also in very advanced stage of programmed cell death, stained with acridine orange (E, G) and with Ag-Nor (F, H). The arrows point to the same nucleolar bodies in slides stained with the two techniques. In H, the highlighted nucleolus is associated with fine, silver stained threads. In C to H, the cytoplasmic material looks very dense. Scale bars: A = 5.0 µm; B, C, D = 7.2 µm; E-H = 11.5 µm.
Mentions: In acridine orange stained slides of late third instar larvae, the chromosomes were green and the nucleolus was orange or red, as expected on the basis of the properties of the stain. The chromatin associated with the nucleolus was well marked, as well as the nucleolus connection with the chromocenter region (Figure 2A). As development progresses, gradual loss of the chromosome-banding pattern, loss of the chromosome individuality, and transformation of the chromosomes into tenuous masses of filaments or tangled threads were observed (Figures 1F, 2B). The nucleolar integrity was also lost in parallel with the loss of its characteristic pattern of silver nitrate impregnation that either became restricted to inner regions of the nucleolus or became irregular (Figure1D, E), unlike the larval stage in which impregnation reaches the whole nucleolar mass. The nucleolar alterations increased until a differentiated nucleolar structure is no longer visible.

Bottom Line: In many cells, the new nucleolar corpuscle and these chromosome threads are associated.These findings are novel.However, the hypothesis put forward concerning their meaning remains dependent on other studies.

View Article: PubMed Central - PubMed

Affiliation: Program in Ecology and Evolutionary Biology, Department of Biological Sciences, University of Arkansas, Fayetteville, AR 72701, USA. cdeolive@uark.edu

ABSTRACT
The salivary glands of Drosophila saltans (saltans group, saltans subgroup) analyzed in an advanced stage of programmed cell death showed the appearance of a single, round, nucleolar corpuscle inside the highly altered nucleus of every gland cell, at a time during which the integrity of the original nucleolus was already lost and the original nucleolar material apparently disappeared. In the same nuclei, which already had also lost the characteristic chromosome structure, some delicate chromosome threads were maintained. In many cells, the new nucleolar corpuscle and these chromosome threads are associated. These findings are novel. However, the hypothesis put forward concerning their meaning remains dependent on other studies.

Show MeSH
Related in: MedlinePlus