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Genomic structure of the luciferase gene from the bioluminescent beetle, Nyctophila cf. caucasica.

Day JC, Chaichi MJ, Najafil I, Whiteley AS - J. Insect Sci. (2006)

Bottom Line: Analysis of the 810 bp upstream region of the luciferase gene revealed three TATA boxes and several other consensus transcriptional factor recognition sequences presenting evidence for a putative core promoter region conserved in Lampyrinae from -190 through to -155 upstream of the luciferase start codon.Along with the core promoter region the luciferase gene was compared with orthologous sequences from other lampyrid species and found to have greatest identity to Lampyris turkistanicus and Lampyris noctiluca.The significant sequence identity to the former is discussed in relation to taxonomic issues of Iranian lampyrids.

View Article: PubMed Central - PubMed

Affiliation: CEH-Oxford, Mansfield Road, Oxford, Oxfordshire OX1 3SR, UK. jcda@ceh.ac.uk

ABSTRACT
The gene coding for beetle luciferase, the enzyme responsible for bioluminescence in over two thousand coleopteran species has, to date, only been characterized from one Palearctic species of Lampyridae. Here we report the characterization of the luciferase gene from a female beetle of an Iranian lampyrid species, Nyctophila cf. caucasica (Coleoptera:Lampyridae). The luciferase gene was composed of seven exons, coding for 547 amino acids, separated by six introns spanning 1976 bp of genomic DNA. The deduced amino acid sequences of the luciferase gene of N. caucasica showed 98.9% homology to that of the Palearctic species Lampyris noctiluca. Analysis of the 810 bp upstream region of the luciferase gene revealed three TATA boxes and several other consensus transcriptional factor recognition sequences presenting evidence for a putative core promoter region conserved in Lampyrinae from -190 through to -155 upstream of the luciferase start codon. Along with the core promoter region the luciferase gene was compared with orthologous sequences from other lampyrid species and found to have greatest identity to Lampyris turkistanicus and Lampyris noctiluca. The significant sequence identity to the former is discussed in relation to taxonomic issues of Iranian lampyrids.

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A phylogenetic tree based upon amino acid sequences of the Nyctophila caucasica luciferase and fifteen known beetle luciferases. The maximum parsimony tree was obtained by a heuristic search with 1000 bootstrap replicates. Branch numbers refer to bootstrap values.
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f05: A phylogenetic tree based upon amino acid sequences of the Nyctophila caucasica luciferase and fifteen known beetle luciferases. The maximum parsimony tree was obtained by a heuristic search with 1000 bootstrap replicates. Branch numbers refer to bootstrap values.

Mentions: The deduced amino acid sequences of the luciferase gene of N. caucasica showed 98.9% homology to that of L. noctiluca (Table 3). Phylogenetic analysis with other bioluminescent beetle luciferases further confirmed that the deduced amino acid sequences of the N. caucasica luciferase gene belonged to the subfamily Lampyrinae (Figure 5). Furthermore, with both Lampyris and Nyctophila along with Pyrocoelia species belonging to Lampyrini the luciferase molecular data supports the taxonomic classification of these species down to the tribe level. However, the most identity shared was with Lampyris turkistanicus, both at a nucleotide level and an amino acid sequence level, 0.992 and 0.998 respectively, that was greater than the sequence identity with species of its own genus (0.981 and 0.987). The strength of the nucleotide sequence identity, 0.992, suggests a misidentification of L. turkistanicus.


Genomic structure of the luciferase gene from the bioluminescent beetle, Nyctophila cf. caucasica.

Day JC, Chaichi MJ, Najafil I, Whiteley AS - J. Insect Sci. (2006)

A phylogenetic tree based upon amino acid sequences of the Nyctophila caucasica luciferase and fifteen known beetle luciferases. The maximum parsimony tree was obtained by a heuristic search with 1000 bootstrap replicates. Branch numbers refer to bootstrap values.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2990329&req=5

f05: A phylogenetic tree based upon amino acid sequences of the Nyctophila caucasica luciferase and fifteen known beetle luciferases. The maximum parsimony tree was obtained by a heuristic search with 1000 bootstrap replicates. Branch numbers refer to bootstrap values.
Mentions: The deduced amino acid sequences of the luciferase gene of N. caucasica showed 98.9% homology to that of L. noctiluca (Table 3). Phylogenetic analysis with other bioluminescent beetle luciferases further confirmed that the deduced amino acid sequences of the N. caucasica luciferase gene belonged to the subfamily Lampyrinae (Figure 5). Furthermore, with both Lampyris and Nyctophila along with Pyrocoelia species belonging to Lampyrini the luciferase molecular data supports the taxonomic classification of these species down to the tribe level. However, the most identity shared was with Lampyris turkistanicus, both at a nucleotide level and an amino acid sequence level, 0.992 and 0.998 respectively, that was greater than the sequence identity with species of its own genus (0.981 and 0.987). The strength of the nucleotide sequence identity, 0.992, suggests a misidentification of L. turkistanicus.

Bottom Line: Analysis of the 810 bp upstream region of the luciferase gene revealed three TATA boxes and several other consensus transcriptional factor recognition sequences presenting evidence for a putative core promoter region conserved in Lampyrinae from -190 through to -155 upstream of the luciferase start codon.Along with the core promoter region the luciferase gene was compared with orthologous sequences from other lampyrid species and found to have greatest identity to Lampyris turkistanicus and Lampyris noctiluca.The significant sequence identity to the former is discussed in relation to taxonomic issues of Iranian lampyrids.

View Article: PubMed Central - PubMed

Affiliation: CEH-Oxford, Mansfield Road, Oxford, Oxfordshire OX1 3SR, UK. jcda@ceh.ac.uk

ABSTRACT
The gene coding for beetle luciferase, the enzyme responsible for bioluminescence in over two thousand coleopteran species has, to date, only been characterized from one Palearctic species of Lampyridae. Here we report the characterization of the luciferase gene from a female beetle of an Iranian lampyrid species, Nyctophila cf. caucasica (Coleoptera:Lampyridae). The luciferase gene was composed of seven exons, coding for 547 amino acids, separated by six introns spanning 1976 bp of genomic DNA. The deduced amino acid sequences of the luciferase gene of N. caucasica showed 98.9% homology to that of the Palearctic species Lampyris noctiluca. Analysis of the 810 bp upstream region of the luciferase gene revealed three TATA boxes and several other consensus transcriptional factor recognition sequences presenting evidence for a putative core promoter region conserved in Lampyrinae from -190 through to -155 upstream of the luciferase start codon. Along with the core promoter region the luciferase gene was compared with orthologous sequences from other lampyrid species and found to have greatest identity to Lampyris turkistanicus and Lampyris noctiluca. The significant sequence identity to the former is discussed in relation to taxonomic issues of Iranian lampyrids.

Show MeSH