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Genomic structure of the luciferase gene from the bioluminescent beetle, Nyctophila cf. caucasica.

Day JC, Chaichi MJ, Najafil I, Whiteley AS - J. Insect Sci. (2006)

Bottom Line: Analysis of the 810 bp upstream region of the luciferase gene revealed three TATA boxes and several other consensus transcriptional factor recognition sequences presenting evidence for a putative core promoter region conserved in Lampyrinae from -190 through to -155 upstream of the luciferase start codon.Along with the core promoter region the luciferase gene was compared with orthologous sequences from other lampyrid species and found to have greatest identity to Lampyris turkistanicus and Lampyris noctiluca.The significant sequence identity to the former is discussed in relation to taxonomic issues of Iranian lampyrids.

View Article: PubMed Central - PubMed

Affiliation: CEH-Oxford, Mansfield Road, Oxford, Oxfordshire OX1 3SR, UK. jcda@ceh.ac.uk

ABSTRACT
The gene coding for beetle luciferase, the enzyme responsible for bioluminescence in over two thousand coleopteran species has, to date, only been characterized from one Palearctic species of Lampyridae. Here we report the characterization of the luciferase gene from a female beetle of an Iranian lampyrid species, Nyctophila cf. caucasica (Coleoptera:Lampyridae). The luciferase gene was composed of seven exons, coding for 547 amino acids, separated by six introns spanning 1976 bp of genomic DNA. The deduced amino acid sequences of the luciferase gene of N. caucasica showed 98.9% homology to that of the Palearctic species Lampyris noctiluca. Analysis of the 810 bp upstream region of the luciferase gene revealed three TATA boxes and several other consensus transcriptional factor recognition sequences presenting evidence for a putative core promoter region conserved in Lampyrinae from -190 through to -155 upstream of the luciferase start codon. Along with the core promoter region the luciferase gene was compared with orthologous sequences from other lampyrid species and found to have greatest identity to Lampyris turkistanicus and Lampyris noctiluca. The significant sequence identity to the former is discussed in relation to taxonomic issues of Iranian lampyrids.

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The 5′-flanking nucleotide sequence of the luciferase gene from Nyctophila caucasica aligned with orthologous sequence from Photinus pyralis (GeneBank accession # M15077). Nucleotides are numbered from the translation initiator ATG (bold) with A being position +1. Two putative TATA boxes and a CCAAT box conserved in both sequences are highlighted in grey.
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f04: The 5′-flanking nucleotide sequence of the luciferase gene from Nyctophila caucasica aligned with orthologous sequence from Photinus pyralis (GeneBank accession # M15077). Nucleotides are numbered from the translation initiator ATG (bold) with A being position +1. Two putative TATA boxes and a CCAAT box conserved in both sequences are highlighted in grey.

Mentions: The 810 bases upstream of the N. caucasica luciferase gene were examined for putative promoter sites. Four TATA boxes, two CCAAT boxes and two GATA motifs were identified in the upstream region (Figure 3). Despite extensive sequence differences, comparisons with P. pyralis luciferase flanking sequence revealed that three motifs were conserved in both species, two TATA boxes at positions -190 and -166 (positions refer to N. caucasica sequence) along with a conserved CCAAT box at position -159 (Figure 4). This suggests the presence of a putative core promoter conserved in Lampyrinae from -190 through to -155. It was not possible to infer a similar core promoter region in members of the Lucolinae due to insufficient identity with Luciola lateralis upstream flanking sequence (GeneBank accession numbers U49182 and U51019).


Genomic structure of the luciferase gene from the bioluminescent beetle, Nyctophila cf. caucasica.

Day JC, Chaichi MJ, Najafil I, Whiteley AS - J. Insect Sci. (2006)

The 5′-flanking nucleotide sequence of the luciferase gene from Nyctophila caucasica aligned with orthologous sequence from Photinus pyralis (GeneBank accession # M15077). Nucleotides are numbered from the translation initiator ATG (bold) with A being position +1. Two putative TATA boxes and a CCAAT box conserved in both sequences are highlighted in grey.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2990329&req=5

f04: The 5′-flanking nucleotide sequence of the luciferase gene from Nyctophila caucasica aligned with orthologous sequence from Photinus pyralis (GeneBank accession # M15077). Nucleotides are numbered from the translation initiator ATG (bold) with A being position +1. Two putative TATA boxes and a CCAAT box conserved in both sequences are highlighted in grey.
Mentions: The 810 bases upstream of the N. caucasica luciferase gene were examined for putative promoter sites. Four TATA boxes, two CCAAT boxes and two GATA motifs were identified in the upstream region (Figure 3). Despite extensive sequence differences, comparisons with P. pyralis luciferase flanking sequence revealed that three motifs were conserved in both species, two TATA boxes at positions -190 and -166 (positions refer to N. caucasica sequence) along with a conserved CCAAT box at position -159 (Figure 4). This suggests the presence of a putative core promoter conserved in Lampyrinae from -190 through to -155. It was not possible to infer a similar core promoter region in members of the Lucolinae due to insufficient identity with Luciola lateralis upstream flanking sequence (GeneBank accession numbers U49182 and U51019).

Bottom Line: Analysis of the 810 bp upstream region of the luciferase gene revealed three TATA boxes and several other consensus transcriptional factor recognition sequences presenting evidence for a putative core promoter region conserved in Lampyrinae from -190 through to -155 upstream of the luciferase start codon.Along with the core promoter region the luciferase gene was compared with orthologous sequences from other lampyrid species and found to have greatest identity to Lampyris turkistanicus and Lampyris noctiluca.The significant sequence identity to the former is discussed in relation to taxonomic issues of Iranian lampyrids.

View Article: PubMed Central - PubMed

Affiliation: CEH-Oxford, Mansfield Road, Oxford, Oxfordshire OX1 3SR, UK. jcda@ceh.ac.uk

ABSTRACT
The gene coding for beetle luciferase, the enzyme responsible for bioluminescence in over two thousand coleopteran species has, to date, only been characterized from one Palearctic species of Lampyridae. Here we report the characterization of the luciferase gene from a female beetle of an Iranian lampyrid species, Nyctophila cf. caucasica (Coleoptera:Lampyridae). The luciferase gene was composed of seven exons, coding for 547 amino acids, separated by six introns spanning 1976 bp of genomic DNA. The deduced amino acid sequences of the luciferase gene of N. caucasica showed 98.9% homology to that of the Palearctic species Lampyris noctiluca. Analysis of the 810 bp upstream region of the luciferase gene revealed three TATA boxes and several other consensus transcriptional factor recognition sequences presenting evidence for a putative core promoter region conserved in Lampyrinae from -190 through to -155 upstream of the luciferase start codon. Along with the core promoter region the luciferase gene was compared with orthologous sequences from other lampyrid species and found to have greatest identity to Lampyris turkistanicus and Lampyris noctiluca. The significant sequence identity to the former is discussed in relation to taxonomic issues of Iranian lampyrids.

Show MeSH