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Infection by Wolbachia bacteria and its influence on the reproduction of the stored-product psocid, Liposcelis tricolor.

Dong P, Wang JJ, Zhao ZM - J. Insect Sci. (2006)

Bottom Line: Phylogenetic analysis showed that the Wolbachia found in L. tricolor was related to the B-group.The results of crosses including female(W+) x male(W+), female(W-) x male(W+), female(W+) x male(W-), and female(W-) x male(W-), suggested that the removal of Wolbachia resulted in lower egg production by L. tricolor .The mean embryonic mortality of offspring produced by L. tricolor without Wolbachia was significantly higher than that of control.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, PR China. dongpeng77@yahoo.com.cn

ABSTRACT
Wolbachia are maternally inherited intracellular bacteria that infect a wide range of arthropods and nematodes and are associated with various reproductive abnormalities in their hosts. The infection by Wolbachia of the psocid, Liposcelis tricolor (Psocoptera: Liposcelididae), was investigated using long PCR amplification of the wsp gene that codes for a Wolbachia surface protein. The results showed that L. tricolor was positive for Wolbachia . Phylogenetic analysis showed that the Wolbachia found in L. tricolor was related to the B-group. Wolbachia infection in L. tricolor could be removed through antibiotic treatment. The results of crosses including female(W+) x male(W+), female(W-) x male(W+), female(W+) x male(W-), and female(W-) x male(W-), suggested that the removal of Wolbachia resulted in lower egg production by L. tricolor . The mean embryonic mortality of offspring produced by L. tricolor without Wolbachia was significantly higher than that of control.

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Long PCR amplification of wsp sequences from L. tricolor treated with antibiotic of different dosages. Lane 1, 100bp DNA marker; Lane 2, 1% rifampicin; lane 3, 0.3% rifampicin; lane 4, 0.03% rifampicin.
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i1536-2442-6-24-1-f03: Long PCR amplification of wsp sequences from L. tricolor treated with antibiotic of different dosages. Lane 1, 100bp DNA marker; Lane 2, 1% rifampicin; lane 3, 0.3% rifampicin; lane 4, 0.03% rifampicin.

Mentions: After 4 weeks treatment with 1% rifampicin, no detectable level of the wsp gene was amplified, while the same gene fragment remained present in the 0.3% or 0.03% rifampicin treated individuals (Fig. 3). A strain of L. tricolor without Wolbachia infection was obtained.


Infection by Wolbachia bacteria and its influence on the reproduction of the stored-product psocid, Liposcelis tricolor.

Dong P, Wang JJ, Zhao ZM - J. Insect Sci. (2006)

Long PCR amplification of wsp sequences from L. tricolor treated with antibiotic of different dosages. Lane 1, 100bp DNA marker; Lane 2, 1% rifampicin; lane 3, 0.3% rifampicin; lane 4, 0.03% rifampicin.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2990312&req=5

i1536-2442-6-24-1-f03: Long PCR amplification of wsp sequences from L. tricolor treated with antibiotic of different dosages. Lane 1, 100bp DNA marker; Lane 2, 1% rifampicin; lane 3, 0.3% rifampicin; lane 4, 0.03% rifampicin.
Mentions: After 4 weeks treatment with 1% rifampicin, no detectable level of the wsp gene was amplified, while the same gene fragment remained present in the 0.3% or 0.03% rifampicin treated individuals (Fig. 3). A strain of L. tricolor without Wolbachia infection was obtained.

Bottom Line: Phylogenetic analysis showed that the Wolbachia found in L. tricolor was related to the B-group.The results of crosses including female(W+) x male(W+), female(W-) x male(W+), female(W+) x male(W-), and female(W-) x male(W-), suggested that the removal of Wolbachia resulted in lower egg production by L. tricolor .The mean embryonic mortality of offspring produced by L. tricolor without Wolbachia was significantly higher than that of control.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, PR China. dongpeng77@yahoo.com.cn

ABSTRACT
Wolbachia are maternally inherited intracellular bacteria that infect a wide range of arthropods and nematodes and are associated with various reproductive abnormalities in their hosts. The infection by Wolbachia of the psocid, Liposcelis tricolor (Psocoptera: Liposcelididae), was investigated using long PCR amplification of the wsp gene that codes for a Wolbachia surface protein. The results showed that L. tricolor was positive for Wolbachia . Phylogenetic analysis showed that the Wolbachia found in L. tricolor was related to the B-group. Wolbachia infection in L. tricolor could be removed through antibiotic treatment. The results of crosses including female(W+) x male(W+), female(W-) x male(W+), female(W+) x male(W-), and female(W-) x male(W-), suggested that the removal of Wolbachia resulted in lower egg production by L. tricolor . The mean embryonic mortality of offspring produced by L. tricolor without Wolbachia was significantly higher than that of control.

Show MeSH
Related in: MedlinePlus