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DNA fingerprinting using AFLP markers to search for markers associated with yield attributes in the silkworm, Bombyx mori.

Gaviria DA, Aguilar E, Serrano HJ, Alegria AH - J. Insect Sci. (2006)

Bottom Line: The molecular markers and the productivity characters were correlated by multiple variance analysis.The analysis permitted the identification of molecular markers associated with the cocoon weight or the shell weight separately.Some markers were associated with both characters.

View Article: PubMed Central - PubMed

Affiliation: Center for Molecular Biology and Biotechnology, Universidad Tecnológica de Pereira, Risaralda, Colombia. duberney82@netscape.net

ABSTRACT
This study was carried out on 11 Chinese and 12 Japanese silkworm strains maintained by the Center for the Technological Development of Sericulture (CDTS) germplasm bank, located in Pereira, Colombia. The goals were to determine the genetic population structure of the two groups and the association between molecular markers (AFLPs) and important productivity characters. Group analysis showed the separation of the strains according to their geographic origin. The molecular markers and the productivity characters were correlated by multiple variance analysis. The analysis permitted the identification of molecular markers associated with the cocoon weight or the shell weight separately. Some markers were associated with both characters.

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Related in: MedlinePlus

Dendrogram obtained by UPGMA indicating the relationships between the 23 CDTS silkworm lines. (168 AFLP bands). Signs (−) and (+) indicate lines with low or high productivity respectively.
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i1536-2442-6-15-1-f01: Dendrogram obtained by UPGMA indicating the relationships between the 23 CDTS silkworm lines. (168 AFLP bands). Signs (−) and (+) indicate lines with low or high productivity respectively.

Mentions: Individual analysis of each of the used combinations gave similar results and presented correlation indices for the data of the different matrices higher than 90%, indicating that the number of combinations used was sufficient for exploring the diversity present in the silkworm lines studied. Since the combinations gave highly concordant results, it is assumed that the whole genome was being analyzed (Barker et al. 1999; Rouppe et al. 1997; Zhu et al. 1998). The dendrograms corresponding to each of the primer combinations gave similar groupings (Figures 1 and 2).


DNA fingerprinting using AFLP markers to search for markers associated with yield attributes in the silkworm, Bombyx mori.

Gaviria DA, Aguilar E, Serrano HJ, Alegria AH - J. Insect Sci. (2006)

Dendrogram obtained by UPGMA indicating the relationships between the 23 CDTS silkworm lines. (168 AFLP bands). Signs (−) and (+) indicate lines with low or high productivity respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2990296&req=5

i1536-2442-6-15-1-f01: Dendrogram obtained by UPGMA indicating the relationships between the 23 CDTS silkworm lines. (168 AFLP bands). Signs (−) and (+) indicate lines with low or high productivity respectively.
Mentions: Individual analysis of each of the used combinations gave similar results and presented correlation indices for the data of the different matrices higher than 90%, indicating that the number of combinations used was sufficient for exploring the diversity present in the silkworm lines studied. Since the combinations gave highly concordant results, it is assumed that the whole genome was being analyzed (Barker et al. 1999; Rouppe et al. 1997; Zhu et al. 1998). The dendrograms corresponding to each of the primer combinations gave similar groupings (Figures 1 and 2).

Bottom Line: The molecular markers and the productivity characters were correlated by multiple variance analysis.The analysis permitted the identification of molecular markers associated with the cocoon weight or the shell weight separately.Some markers were associated with both characters.

View Article: PubMed Central - PubMed

Affiliation: Center for Molecular Biology and Biotechnology, Universidad Tecnológica de Pereira, Risaralda, Colombia. duberney82@netscape.net

ABSTRACT
This study was carried out on 11 Chinese and 12 Japanese silkworm strains maintained by the Center for the Technological Development of Sericulture (CDTS) germplasm bank, located in Pereira, Colombia. The goals were to determine the genetic population structure of the two groups and the association between molecular markers (AFLPs) and important productivity characters. Group analysis showed the separation of the strains according to their geographic origin. The molecular markers and the productivity characters were correlated by multiple variance analysis. The analysis permitted the identification of molecular markers associated with the cocoon weight or the shell weight separately. Some markers were associated with both characters.

Show MeSH
Related in: MedlinePlus