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A transcriptome analysis of the Aedes aegypti vitellogenic fat body.

Feitosa FM, Calvo E, Merino EF, Durham AM, James AA, de Bianchi AG, Marinotti O, Capurro ML - J. Insect Sci. (2006)

Bottom Line: The fat body is a dynamic tissue that participates in multiple biochemical functions of intermediate metabolism.The putative translated proteins were classified into categories based on their function in accordance with significant similarity using the BlastX at NCBI FTP site and Pfam (Bateman et al. 2000) and SMART (Schultz et al. 2000) databases.The characterization of transcripts expressed in the fat body of Ae. aegypti at 24 hours post blood meal provides a basic tool for understanding the processes occurring in this organ and could identify putative new genes whose promoters can be used to specifically express transgenes in the fat bodies of Ae. aegypti.

View Article: PubMed Central - PubMed

Affiliation: Department of Parasitology, Institute of Biomedical Sciences, Universidade de São Paulo, Butantan, São Paulo, SP, Brazil.

ABSTRACT
Aedes (Stegomyia) aegypti is an important dengue vector in tropical and subtropical zones throughout the world. A transcriptome of Ae. aegypti vitellogenic fat bodies is described here. The fat body is a dynamic tissue that participates in multiple biochemical functions of intermediate metabolism. A total of 589 randomly selected cDNAs were assembled into 262 clusters based on their primary sequence similarities. The putative translated proteins were classified into categories based on their function in accordance with significant similarity using the BlastX at NCBI FTP site and Pfam (Bateman et al. 2000) and SMART (Schultz et al. 2000) databases. The characterization of transcripts expressed in the fat body of Ae. aegypti at 24 hours post blood meal provides a basic tool for understanding the processes occurring in this organ and could identify putative new genes whose promoters can be used to specifically express transgenes in the fat bodies of Ae. aegypti.

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Number of sequences of the Aedes aegypti fat body cDNA library. The transcript categories are more represented (A) and less represented (B - Others). The sequences were classified in accordance with function based on similarity of the deduced Ae. aegypti protein sequences deposited in the NR protein database at NCBI. Translated sequences were also screened with RPSBlast for protein motifs of the combined set of Pfam (Bateman et al. 2000) and SMART (Schultz et al 2000) databases. See also Table 2.
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i1536-2442-6-6-1-f01: Number of sequences of the Aedes aegypti fat body cDNA library. The transcript categories are more represented (A) and less represented (B - Others). The sequences were classified in accordance with function based on similarity of the deduced Ae. aegypti protein sequences deposited in the NR protein database at NCBI. Translated sequences were also screened with RPSBlast for protein motifs of the combined set of Pfam (Bateman et al. 2000) and SMART (Schultz et al 2000) databases. See also Table 2.

Mentions: All of the cDNAs reported here were divided into 21 groups according to their predicted function by gene ontology (Ashburner et al. 2000), (Figure 1 and Table 2). In total, 142 sequences distributed in 75 clusters, representing 40.46% of the total of fat body ESTs database, correspond to genes whose products are associated with protein synthesis. This is consistent with the synthetic capability of the fat bodies. The majority of these cDNAs encode ribosomal proteins (67 clusters). The presence of transcripts encoding an elongation factor and a translation initiation factor supports further the conclusion of an abundant protein synthetic activity of this organ.


A transcriptome analysis of the Aedes aegypti vitellogenic fat body.

Feitosa FM, Calvo E, Merino EF, Durham AM, James AA, de Bianchi AG, Marinotti O, Capurro ML - J. Insect Sci. (2006)

Number of sequences of the Aedes aegypti fat body cDNA library. The transcript categories are more represented (A) and less represented (B - Others). The sequences were classified in accordance with function based on similarity of the deduced Ae. aegypti protein sequences deposited in the NR protein database at NCBI. Translated sequences were also screened with RPSBlast for protein motifs of the combined set of Pfam (Bateman et al. 2000) and SMART (Schultz et al 2000) databases. See also Table 2.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2990292&req=5

i1536-2442-6-6-1-f01: Number of sequences of the Aedes aegypti fat body cDNA library. The transcript categories are more represented (A) and less represented (B - Others). The sequences were classified in accordance with function based on similarity of the deduced Ae. aegypti protein sequences deposited in the NR protein database at NCBI. Translated sequences were also screened with RPSBlast for protein motifs of the combined set of Pfam (Bateman et al. 2000) and SMART (Schultz et al 2000) databases. See also Table 2.
Mentions: All of the cDNAs reported here were divided into 21 groups according to their predicted function by gene ontology (Ashburner et al. 2000), (Figure 1 and Table 2). In total, 142 sequences distributed in 75 clusters, representing 40.46% of the total of fat body ESTs database, correspond to genes whose products are associated with protein synthesis. This is consistent with the synthetic capability of the fat bodies. The majority of these cDNAs encode ribosomal proteins (67 clusters). The presence of transcripts encoding an elongation factor and a translation initiation factor supports further the conclusion of an abundant protein synthetic activity of this organ.

Bottom Line: The fat body is a dynamic tissue that participates in multiple biochemical functions of intermediate metabolism.The putative translated proteins were classified into categories based on their function in accordance with significant similarity using the BlastX at NCBI FTP site and Pfam (Bateman et al. 2000) and SMART (Schultz et al. 2000) databases.The characterization of transcripts expressed in the fat body of Ae. aegypti at 24 hours post blood meal provides a basic tool for understanding the processes occurring in this organ and could identify putative new genes whose promoters can be used to specifically express transgenes in the fat bodies of Ae. aegypti.

View Article: PubMed Central - PubMed

Affiliation: Department of Parasitology, Institute of Biomedical Sciences, Universidade de São Paulo, Butantan, São Paulo, SP, Brazil.

ABSTRACT
Aedes (Stegomyia) aegypti is an important dengue vector in tropical and subtropical zones throughout the world. A transcriptome of Ae. aegypti vitellogenic fat bodies is described here. The fat body is a dynamic tissue that participates in multiple biochemical functions of intermediate metabolism. A total of 589 randomly selected cDNAs were assembled into 262 clusters based on their primary sequence similarities. The putative translated proteins were classified into categories based on their function in accordance with significant similarity using the BlastX at NCBI FTP site and Pfam (Bateman et al. 2000) and SMART (Schultz et al. 2000) databases. The characterization of transcripts expressed in the fat body of Ae. aegypti at 24 hours post blood meal provides a basic tool for understanding the processes occurring in this organ and could identify putative new genes whose promoters can be used to specifically express transgenes in the fat bodies of Ae. aegypti.

Show MeSH
Related in: MedlinePlus