Limits...
Incorporation of rhodamine B into male tobacco budworm moths Heliothis virescens to use as a marker for mating studies.

Blanco CA, Perera O, Ray JD, Taliercio E, Williams L - J. Insect Sci. (2006)

Bottom Line: The intake of this dye did not affect the life span, the production of eggs or the capacity of moths to copulate when compared with moths fed only a sucrose solution or water.The dye accumulation in internal abdominal organs was a better indicator of the presence of the pigment than external contamination of the moth.The use of the method described in this report can be a tool for the rapid incorporation of a low cost dye in the tobacco budworm for biological, behavioral and genetic studies.

View Article: PubMed Central - PubMed

Affiliation: USDA-ARS, Southern Insect Management Research Unit, Stoneville, MS 38776, USA. cblanco@ars.usda.gov

ABSTRACT
Rhodamine B, a dye commonly used in a variety of biological studies was incorporated into the bodies of male tobacco budworm moths, Heliothis virescens (Lepidoptera: Noctuidae), by allowing them to feed freely on 0.1% rhodamine dissolved in a 10% sucrose solution. After exposing males for one to three days to this pigment, rhodamine was clearly detectable in >82% of spermatophores extracted from untreated females. The intake of this dye did not affect the life span, the production of eggs or the capacity of moths to copulate when compared with moths fed only a sucrose solution or water. Rhodamine B was easily identifiable externally but was more apparent internally in males after only one day of exposure to the pigment. Even at this short feeding duration, rhodamine was detectable in >50% of males 5 days after feeding stopped. Longer exposure to the dye significantly increased the percentage stained. Detection of rhodamine was slightly enhanced by the use of ultraviolet light. The dye accumulation in internal abdominal organs was a better indicator of the presence of the pigment than external contamination of the moth. The use of the method described in this report can be a tool for the rapid incorporation of a low cost dye in the tobacco budworm for biological, behavioral and genetic studies.

Show MeSH

Related in: MedlinePlus

Average number of eggs (A) and percent fertile eggs (B) per Heliothis virescens female after being mated with males fed different solutions (water, sucrose solution or rhodamine B [0.l%] dissolved in 10% sucrose solution) for l to 4 days. NS = not significantly different at P < 0.05. (N = 60).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2990291&req=5

i1536-2442-6-5-1-f09: Average number of eggs (A) and percent fertile eggs (B) per Heliothis virescens female after being mated with males fed different solutions (water, sucrose solution or rhodamine B [0.l%] dissolved in 10% sucrose solution) for l to 4 days. NS = not significantly different at P < 0.05. (N = 60).

Mentions: Rhodamine detection in frozen moths was evaluated by four different methods. The exterior of the moths was visually inspected (Inspection Method 1, “Visual”) for signs of rhodamine (red coloration) without the use of a magnifying glass or microscope. The same moths were externally inspected under UV light (Spectroline™ 240-C at 254 and 365 nm wavelength) (Inspection Method 2, “UV”). After moths were inspected by methods 1 and 2, moth abdomens were crushed by hand onto a white paper towel to obtain internal fluids. Paper towels with abdomen-fluid stains were visually inspected without (Inspection Method 3, “Paper”) and with (Inspection Method 4, “Paper/UV”) the use of UV-light (254 and 365 nm) (Illustration 2).


Incorporation of rhodamine B into male tobacco budworm moths Heliothis virescens to use as a marker for mating studies.

Blanco CA, Perera O, Ray JD, Taliercio E, Williams L - J. Insect Sci. (2006)

Average number of eggs (A) and percent fertile eggs (B) per Heliothis virescens female after being mated with males fed different solutions (water, sucrose solution or rhodamine B [0.l%] dissolved in 10% sucrose solution) for l to 4 days. NS = not significantly different at P < 0.05. (N = 60).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2990291&req=5

i1536-2442-6-5-1-f09: Average number of eggs (A) and percent fertile eggs (B) per Heliothis virescens female after being mated with males fed different solutions (water, sucrose solution or rhodamine B [0.l%] dissolved in 10% sucrose solution) for l to 4 days. NS = not significantly different at P < 0.05. (N = 60).
Mentions: Rhodamine detection in frozen moths was evaluated by four different methods. The exterior of the moths was visually inspected (Inspection Method 1, “Visual”) for signs of rhodamine (red coloration) without the use of a magnifying glass or microscope. The same moths were externally inspected under UV light (Spectroline™ 240-C at 254 and 365 nm wavelength) (Inspection Method 2, “UV”). After moths were inspected by methods 1 and 2, moth abdomens were crushed by hand onto a white paper towel to obtain internal fluids. Paper towels with abdomen-fluid stains were visually inspected without (Inspection Method 3, “Paper”) and with (Inspection Method 4, “Paper/UV”) the use of UV-light (254 and 365 nm) (Illustration 2).

Bottom Line: The intake of this dye did not affect the life span, the production of eggs or the capacity of moths to copulate when compared with moths fed only a sucrose solution or water.The dye accumulation in internal abdominal organs was a better indicator of the presence of the pigment than external contamination of the moth.The use of the method described in this report can be a tool for the rapid incorporation of a low cost dye in the tobacco budworm for biological, behavioral and genetic studies.

View Article: PubMed Central - PubMed

Affiliation: USDA-ARS, Southern Insect Management Research Unit, Stoneville, MS 38776, USA. cblanco@ars.usda.gov

ABSTRACT
Rhodamine B, a dye commonly used in a variety of biological studies was incorporated into the bodies of male tobacco budworm moths, Heliothis virescens (Lepidoptera: Noctuidae), by allowing them to feed freely on 0.1% rhodamine dissolved in a 10% sucrose solution. After exposing males for one to three days to this pigment, rhodamine was clearly detectable in >82% of spermatophores extracted from untreated females. The intake of this dye did not affect the life span, the production of eggs or the capacity of moths to copulate when compared with moths fed only a sucrose solution or water. Rhodamine B was easily identifiable externally but was more apparent internally in males after only one day of exposure to the pigment. Even at this short feeding duration, rhodamine was detectable in >50% of males 5 days after feeding stopped. Longer exposure to the dye significantly increased the percentage stained. Detection of rhodamine was slightly enhanced by the use of ultraviolet light. The dye accumulation in internal abdominal organs was a better indicator of the presence of the pigment than external contamination of the moth. The use of the method described in this report can be a tool for the rapid incorporation of a low cost dye in the tobacco budworm for biological, behavioral and genetic studies.

Show MeSH
Related in: MedlinePlus