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Serum microRNA characterization identifies miR-885-5p as a potential marker for detecting liver pathologies.

Gui J, Tian Y, Wen X, Zhang W, Zhang P, Gao J, Run W, Tian L, Jia X, Gao Y - Clin. Sci. (2011)

Bottom Line: Five miRNAs (i.e. miR-885-5p, miR-574-3p, miR-224, miR-215 and miR-146a) that were up-regulated in the HCC and LC serum pools were selected and further quantified using real-time qPCR in patients with HCC, LC, CHB (chronic hepatitis B) or GC (gastric cancer) and in normal controls.The present study revealed that more than 110 miRNA species in the serum samples and wide distribution ranges of serum miRNAs were observed.No correlations between increased miR-885-5p and liver function parameters [AFP (α-fetoprotein), ALT (alanine aminotransferase), AST (aspartate aminotransferase) and GGT (γ-glutamyl transpeptidase)] were observed in patients with liver pathologies.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Biochemistry, Chinese PLA General Hospital, 28 Fuxing Rd, Beijing 100853, People's Republic of China.

ABSTRACT
Circulating miRNAs (microRNAs) are emerging as promising biomarkers for several pathological conditions, and the aim of this study was to investigate the feasibility of using serum miRNAs as biomarkers for liver pathologies. Real-time qPCR (quantitative PCR)-based TaqMan MicroRNA arrays were first employed to profile miRNAs in serum pools from patients with HCC (hepatocellular carcinoma) or LC (liver cirrhosis) and from healthy controls. Five miRNAs (i.e. miR-885-5p, miR-574-3p, miR-224, miR-215 and miR-146a) that were up-regulated in the HCC and LC serum pools were selected and further quantified using real-time qPCR in patients with HCC, LC, CHB (chronic hepatitis B) or GC (gastric cancer) and in normal controls. The present study revealed that more than 110 miRNA species in the serum samples and wide distribution ranges of serum miRNAs were observed. The levels of miR-885-5p were significantly higher in sera from patients with HCC, LC and CHB than in healthy controls or GC patients. miR-885-5p yielded an AUC [the area under the ROC (receiver operating characteristic) curve] of 0.904 [95% CI (confidence interval), 0.837-0.951, P<0.0001) with 90.53% sensitivity and 79.17% specificity in discriminating liver pathologies from healthy controls, using a cut off value of 1.06 (normalized). No correlations between increased miR-885-5p and liver function parameters [AFP (α-fetoprotein), ALT (alanine aminotransferase), AST (aspartate aminotransferase) and GGT (γ-glutamyl transpeptidase)] were observed in patients with liver pathologies. In summary, miR-885-5p is significantly elevated in the sera of patients with liver pathologies, and our data suggest that serum miRNAs could serve as novel complementary biomarkers for the detection and assessment of liver pathologies.

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Related in: MedlinePlus

Expression of 115 miRNAs in representative serum pools from the NC, LC and HCC groupsmiRNAs were quantified using RT-preamp-qPCR as described in the Materials and methods section. The relative abundance of each miRNA is presented as 2−ΔCt, where ΔCt was calculated by subtracting the Ct values of Mamm U6 (28.1 in NC, 29.4 in LC and 30.0 in HCC pool, respectively) from the Ct of each miRNA detected within the corresponding groups. The levels of various miRNAs are shown (normalized) in representative NC, LC and HCC serum pools.
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Figure 1: Expression of 115 miRNAs in representative serum pools from the NC, LC and HCC groupsmiRNAs were quantified using RT-preamp-qPCR as described in the Materials and methods section. The relative abundance of each miRNA is presented as 2−ΔCt, where ΔCt was calculated by subtracting the Ct values of Mamm U6 (28.1 in NC, 29.4 in LC and 30.0 in HCC pool, respectively) from the Ct of each miRNA detected within the corresponding groups. The levels of various miRNAs are shown (normalized) in representative NC, LC and HCC serum pools.

Mentions: To investigate the relative abundances of the miRNAs detected, they were normalized in each serum pool (NC, LC and HCC) to Mamm U6 in the corresponding pool. As shown in Figure 1, miRNAs such as miR-223, miR-16 and miR-146a were present in relatively high abundance in the serum samples, while miR-99a, miR-224, miR-192, miR-128 and miR-100 were present at significantly lower abundance compared with the corresponding Mamm U6 (28.1 in NC, 29.4 in LC and 30.0 in HCC pool, respectively). In addition, the abundances of different miRNA species in a specified serum pool varied widely. For example, the relative abundances (normalized to Mamm U6) of miR-223 and miR-100 in the NC serum pool were 295.09 and 0.00026, respectively.


Serum microRNA characterization identifies miR-885-5p as a potential marker for detecting liver pathologies.

Gui J, Tian Y, Wen X, Zhang W, Zhang P, Gao J, Run W, Tian L, Jia X, Gao Y - Clin. Sci. (2011)

Expression of 115 miRNAs in representative serum pools from the NC, LC and HCC groupsmiRNAs were quantified using RT-preamp-qPCR as described in the Materials and methods section. The relative abundance of each miRNA is presented as 2−ΔCt, where ΔCt was calculated by subtracting the Ct values of Mamm U6 (28.1 in NC, 29.4 in LC and 30.0 in HCC pool, respectively) from the Ct of each miRNA detected within the corresponding groups. The levels of various miRNAs are shown (normalized) in representative NC, LC and HCC serum pools.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2990200&req=5

Figure 1: Expression of 115 miRNAs in representative serum pools from the NC, LC and HCC groupsmiRNAs were quantified using RT-preamp-qPCR as described in the Materials and methods section. The relative abundance of each miRNA is presented as 2−ΔCt, where ΔCt was calculated by subtracting the Ct values of Mamm U6 (28.1 in NC, 29.4 in LC and 30.0 in HCC pool, respectively) from the Ct of each miRNA detected within the corresponding groups. The levels of various miRNAs are shown (normalized) in representative NC, LC and HCC serum pools.
Mentions: To investigate the relative abundances of the miRNAs detected, they were normalized in each serum pool (NC, LC and HCC) to Mamm U6 in the corresponding pool. As shown in Figure 1, miRNAs such as miR-223, miR-16 and miR-146a were present in relatively high abundance in the serum samples, while miR-99a, miR-224, miR-192, miR-128 and miR-100 were present at significantly lower abundance compared with the corresponding Mamm U6 (28.1 in NC, 29.4 in LC and 30.0 in HCC pool, respectively). In addition, the abundances of different miRNA species in a specified serum pool varied widely. For example, the relative abundances (normalized to Mamm U6) of miR-223 and miR-100 in the NC serum pool were 295.09 and 0.00026, respectively.

Bottom Line: Five miRNAs (i.e. miR-885-5p, miR-574-3p, miR-224, miR-215 and miR-146a) that were up-regulated in the HCC and LC serum pools were selected and further quantified using real-time qPCR in patients with HCC, LC, CHB (chronic hepatitis B) or GC (gastric cancer) and in normal controls.The present study revealed that more than 110 miRNA species in the serum samples and wide distribution ranges of serum miRNAs were observed.No correlations between increased miR-885-5p and liver function parameters [AFP (α-fetoprotein), ALT (alanine aminotransferase), AST (aspartate aminotransferase) and GGT (γ-glutamyl transpeptidase)] were observed in patients with liver pathologies.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Biochemistry, Chinese PLA General Hospital, 28 Fuxing Rd, Beijing 100853, People's Republic of China.

ABSTRACT
Circulating miRNAs (microRNAs) are emerging as promising biomarkers for several pathological conditions, and the aim of this study was to investigate the feasibility of using serum miRNAs as biomarkers for liver pathologies. Real-time qPCR (quantitative PCR)-based TaqMan MicroRNA arrays were first employed to profile miRNAs in serum pools from patients with HCC (hepatocellular carcinoma) or LC (liver cirrhosis) and from healthy controls. Five miRNAs (i.e. miR-885-5p, miR-574-3p, miR-224, miR-215 and miR-146a) that were up-regulated in the HCC and LC serum pools were selected and further quantified using real-time qPCR in patients with HCC, LC, CHB (chronic hepatitis B) or GC (gastric cancer) and in normal controls. The present study revealed that more than 110 miRNA species in the serum samples and wide distribution ranges of serum miRNAs were observed. The levels of miR-885-5p were significantly higher in sera from patients with HCC, LC and CHB than in healthy controls or GC patients. miR-885-5p yielded an AUC [the area under the ROC (receiver operating characteristic) curve] of 0.904 [95% CI (confidence interval), 0.837-0.951, P<0.0001) with 90.53% sensitivity and 79.17% specificity in discriminating liver pathologies from healthy controls, using a cut off value of 1.06 (normalized). No correlations between increased miR-885-5p and liver function parameters [AFP (α-fetoprotein), ALT (alanine aminotransferase), AST (aspartate aminotransferase) and GGT (γ-glutamyl transpeptidase)] were observed in patients with liver pathologies. In summary, miR-885-5p is significantly elevated in the sera of patients with liver pathologies, and our data suggest that serum miRNAs could serve as novel complementary biomarkers for the detection and assessment of liver pathologies.

Show MeSH
Related in: MedlinePlus