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Spatio-temporal expression of a novel neuron-derived neurotrophic factor (NDNF) in mouse brains during development.

Kuang XL, Zhao XM, Xu HF, Shi YY, Deng JB, Sun GT - BMC Neurosci (2010)

Bottom Line: Cajal-Retzius cells, cortex neurons, hippocampus neurons, olfactory mitral cells, cerebellar purkinje cells, cerebellar granular cells and spinal neurons were found to be NDNF-positive.NDNF expression was observed in the neurons during development.The results of this study indicated that NDNF is a novel neurotrophic factor derived from neurons that may be useful in the treatment of neuronal degeneration diseases and nerve injuries.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, Institute of Molecular Medicine, Medical School, Henan University, KaiFeng, PR China.

ABSTRACT

Background: Neuron-derived neurotrophic factor (NDNF) is evolutionarily well conserved, being present in invertebrate animals such as the nematode, Caenorhabditis elegans, as well as in the fruit fly, Drosophila melanogaster. Multiple cysteines are conserved between species and secondary structure prediction shows that NDNF is mainly composed of beta-strands. In this study, we aimed to investigate the function of NDNF.

Results: NDNF is a glycosylated, disulfide-bonded secretory protein that contains a fibronectin type III domain. NDNF promoted migration and growth and elicited neurite outgrowth of mouse hippocampal neurons in culture. NDNF also protected cultured hippocampal neurons against excitotoxicity and amyloid beta-peptide toxicity. Western blotting showed that NDNF was exclusively expressed in the brain and spinal cord. Immunostaining indicated that NDNF was expressed by neurons and not by astrocytes. Cajal-Retzius cells, cortex neurons, hippocampus neurons, olfactory mitral cells, cerebellar purkinje cells, cerebellar granular cells and spinal neurons were found to be NDNF-positive. NDNF expression was observed in the neurons during development.

Conclusions: The results of this study indicated that NDNF is a novel neurotrophic factor derived from neurons that may be useful in the treatment of neuronal degeneration diseases and nerve injuries.

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Glycosylation, and tissue distribution of NDNF. A. Mouse NDNF protein immunoprecipitated from brain extracts was incubated with PNGase F and then analyzed by western blot in triplicate. B. Protein samples from the brains, spinal cords, kidneys, livers and hearts of mice were analyzed by western blot analysis using beta-actin as a control in triplicate. NDNF is expressed by cells in the mouse brain and spinal cord.
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Figure 4: Glycosylation, and tissue distribution of NDNF. A. Mouse NDNF protein immunoprecipitated from brain extracts was incubated with PNGase F and then analyzed by western blot in triplicate. B. Protein samples from the brains, spinal cords, kidneys, livers and hearts of mice were analyzed by western blot analysis using beta-actin as a control in triplicate. NDNF is expressed by cells in the mouse brain and spinal cord.

Mentions: Glycosylation is a very common phenomenon among transmembrane and secreted proteins. These glycoproteins are involved in cell-cell or cell-matrix interactions, or regulation of cell surface receptors and hormone functions. To assess glycosylation of NDNF, NDNF was immunoprecipitated from mouse brain extracts and then digested with endoglycosidases PNGase F. Western blots showed that the digested products migrated at a slightly lower molecular weight than the undigested protein, indicating the presence of fairly small or few oligosaccharide groups. These findings are consistent with the two predicted N-linked glycosylation sites (Figure 4A).


Spatio-temporal expression of a novel neuron-derived neurotrophic factor (NDNF) in mouse brains during development.

Kuang XL, Zhao XM, Xu HF, Shi YY, Deng JB, Sun GT - BMC Neurosci (2010)

Glycosylation, and tissue distribution of NDNF. A. Mouse NDNF protein immunoprecipitated from brain extracts was incubated with PNGase F and then analyzed by western blot in triplicate. B. Protein samples from the brains, spinal cords, kidneys, livers and hearts of mice were analyzed by western blot analysis using beta-actin as a control in triplicate. NDNF is expressed by cells in the mouse brain and spinal cord.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2984559&req=5

Figure 4: Glycosylation, and tissue distribution of NDNF. A. Mouse NDNF protein immunoprecipitated from brain extracts was incubated with PNGase F and then analyzed by western blot in triplicate. B. Protein samples from the brains, spinal cords, kidneys, livers and hearts of mice were analyzed by western blot analysis using beta-actin as a control in triplicate. NDNF is expressed by cells in the mouse brain and spinal cord.
Mentions: Glycosylation is a very common phenomenon among transmembrane and secreted proteins. These glycoproteins are involved in cell-cell or cell-matrix interactions, or regulation of cell surface receptors and hormone functions. To assess glycosylation of NDNF, NDNF was immunoprecipitated from mouse brain extracts and then digested with endoglycosidases PNGase F. Western blots showed that the digested products migrated at a slightly lower molecular weight than the undigested protein, indicating the presence of fairly small or few oligosaccharide groups. These findings are consistent with the two predicted N-linked glycosylation sites (Figure 4A).

Bottom Line: Cajal-Retzius cells, cortex neurons, hippocampus neurons, olfactory mitral cells, cerebellar purkinje cells, cerebellar granular cells and spinal neurons were found to be NDNF-positive.NDNF expression was observed in the neurons during development.The results of this study indicated that NDNF is a novel neurotrophic factor derived from neurons that may be useful in the treatment of neuronal degeneration diseases and nerve injuries.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, Institute of Molecular Medicine, Medical School, Henan University, KaiFeng, PR China.

ABSTRACT

Background: Neuron-derived neurotrophic factor (NDNF) is evolutionarily well conserved, being present in invertebrate animals such as the nematode, Caenorhabditis elegans, as well as in the fruit fly, Drosophila melanogaster. Multiple cysteines are conserved between species and secondary structure prediction shows that NDNF is mainly composed of beta-strands. In this study, we aimed to investigate the function of NDNF.

Results: NDNF is a glycosylated, disulfide-bonded secretory protein that contains a fibronectin type III domain. NDNF promoted migration and growth and elicited neurite outgrowth of mouse hippocampal neurons in culture. NDNF also protected cultured hippocampal neurons against excitotoxicity and amyloid beta-peptide toxicity. Western blotting showed that NDNF was exclusively expressed in the brain and spinal cord. Immunostaining indicated that NDNF was expressed by neurons and not by astrocytes. Cajal-Retzius cells, cortex neurons, hippocampus neurons, olfactory mitral cells, cerebellar purkinje cells, cerebellar granular cells and spinal neurons were found to be NDNF-positive. NDNF expression was observed in the neurons during development.

Conclusions: The results of this study indicated that NDNF is a novel neurotrophic factor derived from neurons that may be useful in the treatment of neuronal degeneration diseases and nerve injuries.

Show MeSH
Related in: MedlinePlus