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Pseudomonas aeruginosa biofilm matrix polysaccharide Psl is regulated transcriptionally by RpoS and post-transcriptionally by RsmA.

Irie Y, Starkey M, Edwards AN, Wozniak DJ, Romeo T, Parsek MR - Mol. Microbiol. (2010)

Bottom Line: In this study, we demonstrate that the alternative σ-factor RpoS is a positive transcriptional regulator of psl gene expression.Furthermore, we show that psl mRNA has an extensive 5' untranslated region, to which the post-transcriptional regulator RsmA binds and represses psl translation.This constitutes a novel mechanism for translational repression by this family of regulators.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University of Washington, Seattle, WA 98195, USA.

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RsmA is a post-transcriptional regulator of psl.A. Transcriptional lacZ fusion constructs in WT and ΔrsmA backgrounds reveal no significant differences in psl transcription.B. Translational lacZ fusion constructs in WT and ΔrsmA backgrounds show an increase of psl translational activity in the ΔrsmA background. RsmA regulation of psl is independent of the expression of Pel and Psl as indicated by the increase of psl translation in ΔrsmAΔpelΔpsl compared with ΔpelΔpsl, suggesting that this is not an effect from the autoaggregation seen in the delta rsmA strain.C. Translational lacZ fusion constructs in WT and ΔrsmA backgrounds with pUCP18 vector control (VC) and RsmA overexpression plasmid (RsmA ox) result in a decrease of psl translation when RsmA is overexpressed.
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fig06: RsmA is a post-transcriptional regulator of psl.A. Transcriptional lacZ fusion constructs in WT and ΔrsmA backgrounds reveal no significant differences in psl transcription.B. Translational lacZ fusion constructs in WT and ΔrsmA backgrounds show an increase of psl translational activity in the ΔrsmA background. RsmA regulation of psl is independent of the expression of Pel and Psl as indicated by the increase of psl translation in ΔrsmAΔpelΔpsl compared with ΔpelΔpsl, suggesting that this is not an effect from the autoaggregation seen in the delta rsmA strain.C. Translational lacZ fusion constructs in WT and ΔrsmA backgrounds with pUCP18 vector control (VC) and RsmA overexpression plasmid (RsmA ox) result in a decrease of psl translation when RsmA is overexpressed.

Mentions: Translational fusion data showed that psl translation was threefold to fourfold higher in ΔrsmA than WT (Figs 6B and S2B), despite psl transcriptional activity being similar (Figs 6A and S2A). Elevated psl translational activity in ΔrsmA could be complemented by overexpressing RsmA (Fig. 6C). RsmA overexpression further reduced the activity below the WT level, providing additional evidence that RsmA acts as a repressor of psl.


Pseudomonas aeruginosa biofilm matrix polysaccharide Psl is regulated transcriptionally by RpoS and post-transcriptionally by RsmA.

Irie Y, Starkey M, Edwards AN, Wozniak DJ, Romeo T, Parsek MR - Mol. Microbiol. (2010)

RsmA is a post-transcriptional regulator of psl.A. Transcriptional lacZ fusion constructs in WT and ΔrsmA backgrounds reveal no significant differences in psl transcription.B. Translational lacZ fusion constructs in WT and ΔrsmA backgrounds show an increase of psl translational activity in the ΔrsmA background. RsmA regulation of psl is independent of the expression of Pel and Psl as indicated by the increase of psl translation in ΔrsmAΔpelΔpsl compared with ΔpelΔpsl, suggesting that this is not an effect from the autoaggregation seen in the delta rsmA strain.C. Translational lacZ fusion constructs in WT and ΔrsmA backgrounds with pUCP18 vector control (VC) and RsmA overexpression plasmid (RsmA ox) result in a decrease of psl translation when RsmA is overexpressed.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2984543&req=5

fig06: RsmA is a post-transcriptional regulator of psl.A. Transcriptional lacZ fusion constructs in WT and ΔrsmA backgrounds reveal no significant differences in psl transcription.B. Translational lacZ fusion constructs in WT and ΔrsmA backgrounds show an increase of psl translational activity in the ΔrsmA background. RsmA regulation of psl is independent of the expression of Pel and Psl as indicated by the increase of psl translation in ΔrsmAΔpelΔpsl compared with ΔpelΔpsl, suggesting that this is not an effect from the autoaggregation seen in the delta rsmA strain.C. Translational lacZ fusion constructs in WT and ΔrsmA backgrounds with pUCP18 vector control (VC) and RsmA overexpression plasmid (RsmA ox) result in a decrease of psl translation when RsmA is overexpressed.
Mentions: Translational fusion data showed that psl translation was threefold to fourfold higher in ΔrsmA than WT (Figs 6B and S2B), despite psl transcriptional activity being similar (Figs 6A and S2A). Elevated psl translational activity in ΔrsmA could be complemented by overexpressing RsmA (Fig. 6C). RsmA overexpression further reduced the activity below the WT level, providing additional evidence that RsmA acts as a repressor of psl.

Bottom Line: In this study, we demonstrate that the alternative σ-factor RpoS is a positive transcriptional regulator of psl gene expression.Furthermore, we show that psl mRNA has an extensive 5' untranslated region, to which the post-transcriptional regulator RsmA binds and represses psl translation.This constitutes a novel mechanism for translational repression by this family of regulators.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University of Washington, Seattle, WA 98195, USA.

Show MeSH