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Angiogenic factor-enriched platelet-rich plasma enhances in vivo bone formation around alloplastic graft material.

Kim ES, Kim JJ, Park EJ - J Adv Prosthodont (2010)

Bottom Line: In vitro, this human angiogenic factors-enriched PRP resulted in better cellular proliferation and osteogenic differentiation.In vivo, increasing angiogenic potential of the PRP showed significantly higher blood perfusion around the defect and enhanced new bone formation around acellular bone graft material.The results implicate that rapid angiogenesis in the initial healing period by PRP could be supposed as a way to overcome short term effect of the rapid angiogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral & Maxillofacial Surgery, College of Medicine, Chungnam National University, Daejeon, Korea.

ABSTRACT

Purpose: Although most researchers agree that platelet-rich plasma (PRP) is a good source of autogenous growth factors, its effect on bone regeneration is still controversial. The purpose of this study was to evaluate whether increasing angiogenic factors in the human PRP to enhance new bone formation through rapid angiogenesis.

Material and methods: In vitro, the human platelets were activated with application of shear stress, 20 µg/ml collagen, 2 mM CaCl(2) and 10U thrombin/1 × 10(9) platelets. Level of vascular endothelial growth factor (VEGF) and platelet microparticle (PMP) in the activated platelets were checked. In the animal study, human angiogenic factors-enriched PRP was tested in 28 athymic rat's cranial critical bone defects with β-TCP. Angiogenesis and osteogenesis were evaluated by laser Doppler perfusion imaging, histology, dual energy X-ray densinometry, and micro-computed tomography.

Results: In vitro, this human angiogenic factors-enriched PRP resulted in better cellular proliferation and osteogenic differentiation. In vivo, increasing angiogenic potential of the PRP showed significantly higher blood perfusion around the defect and enhanced new bone formation around acellular bone graft material.

Conclusion: Angiogenic factor-enriched PRP leads to faster and more extensive new bone formation in the critical size bone defect. The results implicate that rapid angiogenesis in the initial healing period by PRP could be supposed as a way to overcome short term effect of the rapid angiogenesis.

No MeSH data available.


Related in: MedlinePlus

Photomicrographs of histological sections from critical size 8 mm rat craniotomy defects following implantation of β-TCP (Synthograft®) and several types of PRP at 7 weeks. A: β-TCP only, B: β-TCP and 0.1% triton-X treated PRP, C: β-TCP and PRP activated with 142.8 U/ml thrombin and 4.3 mg/ml CaCl2, D: β-TCP and angiogenic factorsenriched PRP (activated with shear force, 20 mg/ml collagen, 10 U/ml thrombin and 2 mM CaCl2 and containing peripheral blood mononuclear cells).
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Figure 5: Photomicrographs of histological sections from critical size 8 mm rat craniotomy defects following implantation of β-TCP (Synthograft®) and several types of PRP at 7 weeks. A: β-TCP only, B: β-TCP and 0.1% triton-X treated PRP, C: β-TCP and PRP activated with 142.8 U/ml thrombin and 4.3 mg/ml CaCl2, D: β-TCP and angiogenic factorsenriched PRP (activated with shear force, 20 mg/ml collagen, 10 U/ml thrombin and 2 mM CaCl2 and containing peripheral blood mononuclear cells).

Mentions: Analysis of cranial implants indicated that all groups showed favorable tissue response without significant inflammatory reaction (Fig. 5). Human angiogenic factors-enriched PRP (Group D) led to significantly increased BMC and BMD versus control defects grafted with β-TCP only (Group A, P < .05) (Fig. 6). Although some control groups with PRPs (Group B and C) showed higher BMC and BMD values compared to the group grafted with β-TCP only (Group A), they were not statistically significant. These findings were confirmed by 3D µCT imaging, which revealed the formation of a continuous bone mass across the defects (Fig. 7). The control condition without any PRPs demonstrated significantly decreased bone formation.


Angiogenic factor-enriched platelet-rich plasma enhances in vivo bone formation around alloplastic graft material.

Kim ES, Kim JJ, Park EJ - J Adv Prosthodont (2010)

Photomicrographs of histological sections from critical size 8 mm rat craniotomy defects following implantation of β-TCP (Synthograft®) and several types of PRP at 7 weeks. A: β-TCP only, B: β-TCP and 0.1% triton-X treated PRP, C: β-TCP and PRP activated with 142.8 U/ml thrombin and 4.3 mg/ml CaCl2, D: β-TCP and angiogenic factorsenriched PRP (activated with shear force, 20 mg/ml collagen, 10 U/ml thrombin and 2 mM CaCl2 and containing peripheral blood mononuclear cells).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2984511&req=5

Figure 5: Photomicrographs of histological sections from critical size 8 mm rat craniotomy defects following implantation of β-TCP (Synthograft®) and several types of PRP at 7 weeks. A: β-TCP only, B: β-TCP and 0.1% triton-X treated PRP, C: β-TCP and PRP activated with 142.8 U/ml thrombin and 4.3 mg/ml CaCl2, D: β-TCP and angiogenic factorsenriched PRP (activated with shear force, 20 mg/ml collagen, 10 U/ml thrombin and 2 mM CaCl2 and containing peripheral blood mononuclear cells).
Mentions: Analysis of cranial implants indicated that all groups showed favorable tissue response without significant inflammatory reaction (Fig. 5). Human angiogenic factors-enriched PRP (Group D) led to significantly increased BMC and BMD versus control defects grafted with β-TCP only (Group A, P < .05) (Fig. 6). Although some control groups with PRPs (Group B and C) showed higher BMC and BMD values compared to the group grafted with β-TCP only (Group A), they were not statistically significant. These findings were confirmed by 3D µCT imaging, which revealed the formation of a continuous bone mass across the defects (Fig. 7). The control condition without any PRPs demonstrated significantly decreased bone formation.

Bottom Line: In vitro, this human angiogenic factors-enriched PRP resulted in better cellular proliferation and osteogenic differentiation.In vivo, increasing angiogenic potential of the PRP showed significantly higher blood perfusion around the defect and enhanced new bone formation around acellular bone graft material.The results implicate that rapid angiogenesis in the initial healing period by PRP could be supposed as a way to overcome short term effect of the rapid angiogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral & Maxillofacial Surgery, College of Medicine, Chungnam National University, Daejeon, Korea.

ABSTRACT

Purpose: Although most researchers agree that platelet-rich plasma (PRP) is a good source of autogenous growth factors, its effect on bone regeneration is still controversial. The purpose of this study was to evaluate whether increasing angiogenic factors in the human PRP to enhance new bone formation through rapid angiogenesis.

Material and methods: In vitro, the human platelets were activated with application of shear stress, 20 µg/ml collagen, 2 mM CaCl(2) and 10U thrombin/1 × 10(9) platelets. Level of vascular endothelial growth factor (VEGF) and platelet microparticle (PMP) in the activated platelets were checked. In the animal study, human angiogenic factors-enriched PRP was tested in 28 athymic rat's cranial critical bone defects with β-TCP. Angiogenesis and osteogenesis were evaluated by laser Doppler perfusion imaging, histology, dual energy X-ray densinometry, and micro-computed tomography.

Results: In vitro, this human angiogenic factors-enriched PRP resulted in better cellular proliferation and osteogenic differentiation. In vivo, increasing angiogenic potential of the PRP showed significantly higher blood perfusion around the defect and enhanced new bone formation around acellular bone graft material.

Conclusion: Angiogenic factor-enriched PRP leads to faster and more extensive new bone formation in the critical size bone defect. The results implicate that rapid angiogenesis in the initial healing period by PRP could be supposed as a way to overcome short term effect of the rapid angiogenesis.

No MeSH data available.


Related in: MedlinePlus