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Angiogenic factor-enriched platelet-rich plasma enhances in vivo bone formation around alloplastic graft material.

Kim ES, Kim JJ, Park EJ - J Adv Prosthodont (2010)

Bottom Line: In vitro, this human angiogenic factors-enriched PRP resulted in better cellular proliferation and osteogenic differentiation.In vivo, increasing angiogenic potential of the PRP showed significantly higher blood perfusion around the defect and enhanced new bone formation around acellular bone graft material.The results implicate that rapid angiogenesis in the initial healing period by PRP could be supposed as a way to overcome short term effect of the rapid angiogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral & Maxillofacial Surgery, College of Medicine, Chungnam National University, Daejeon, Korea.

ABSTRACT

Purpose: Although most researchers agree that platelet-rich plasma (PRP) is a good source of autogenous growth factors, its effect on bone regeneration is still controversial. The purpose of this study was to evaluate whether increasing angiogenic factors in the human PRP to enhance new bone formation through rapid angiogenesis.

Material and methods: In vitro, the human platelets were activated with application of shear stress, 20 µg/ml collagen, 2 mM CaCl(2) and 10U thrombin/1 × 10(9) platelets. Level of vascular endothelial growth factor (VEGF) and platelet microparticle (PMP) in the activated platelets were checked. In the animal study, human angiogenic factors-enriched PRP was tested in 28 athymic rat's cranial critical bone defects with β-TCP. Angiogenesis and osteogenesis were evaluated by laser Doppler perfusion imaging, histology, dual energy X-ray densinometry, and micro-computed tomography.

Results: In vitro, this human angiogenic factors-enriched PRP resulted in better cellular proliferation and osteogenic differentiation. In vivo, increasing angiogenic potential of the PRP showed significantly higher blood perfusion around the defect and enhanced new bone formation around acellular bone graft material.

Conclusion: Angiogenic factor-enriched PRP leads to faster and more extensive new bone formation in the critical size bone defect. The results implicate that rapid angiogenesis in the initial healing period by PRP could be supposed as a way to overcome short term effect of the rapid angiogenesis.

No MeSH data available.


Related in: MedlinePlus

Staining for vWF in critical size (8 mm) rat craniotomy defects following implantation of β-TCP only (A) and β-TCP + angiogenic factorsenriched PRP (A-PRP, B) at 2 weeks. Blood vessels are identified as circular structure with dark brown color: Quantitative analysis of the number of blood vessels in both groups (C). Values represent the mean and standard deviation.
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Figure 4: Staining for vWF in critical size (8 mm) rat craniotomy defects following implantation of β-TCP only (A) and β-TCP + angiogenic factorsenriched PRP (A-PRP, B) at 2 weeks. Blood vessels are identified as circular structure with dark brown color: Quantitative analysis of the number of blood vessels in both groups (C). Values represent the mean and standard deviation.

Mentions: β-TCP without or with various types of human PRPs were implanted into the cranial defects of athymic rats to determine the effects of human PRP treatment on wound angiogenesis (Fig. 3). A significant increase of blood perfusion was observed in the defects treated with angiogenic factors-enriched PRP at 2 weeks, compared to other conditions (31 - 42% improvement, P < .05). The blood vessel densities within the defects compared to the β-TCP only grafts (Group A, 48 ± 18 vessels/cm2), showed significantly higher neovascularization in the experimental group (Group D, 98 ± 20 vessel/cm2; P < .05) (Fig. 4A-C).


Angiogenic factor-enriched platelet-rich plasma enhances in vivo bone formation around alloplastic graft material.

Kim ES, Kim JJ, Park EJ - J Adv Prosthodont (2010)

Staining for vWF in critical size (8 mm) rat craniotomy defects following implantation of β-TCP only (A) and β-TCP + angiogenic factorsenriched PRP (A-PRP, B) at 2 weeks. Blood vessels are identified as circular structure with dark brown color: Quantitative analysis of the number of blood vessels in both groups (C). Values represent the mean and standard deviation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2984511&req=5

Figure 4: Staining for vWF in critical size (8 mm) rat craniotomy defects following implantation of β-TCP only (A) and β-TCP + angiogenic factorsenriched PRP (A-PRP, B) at 2 weeks. Blood vessels are identified as circular structure with dark brown color: Quantitative analysis of the number of blood vessels in both groups (C). Values represent the mean and standard deviation.
Mentions: β-TCP without or with various types of human PRPs were implanted into the cranial defects of athymic rats to determine the effects of human PRP treatment on wound angiogenesis (Fig. 3). A significant increase of blood perfusion was observed in the defects treated with angiogenic factors-enriched PRP at 2 weeks, compared to other conditions (31 - 42% improvement, P < .05). The blood vessel densities within the defects compared to the β-TCP only grafts (Group A, 48 ± 18 vessels/cm2), showed significantly higher neovascularization in the experimental group (Group D, 98 ± 20 vessel/cm2; P < .05) (Fig. 4A-C).

Bottom Line: In vitro, this human angiogenic factors-enriched PRP resulted in better cellular proliferation and osteogenic differentiation.In vivo, increasing angiogenic potential of the PRP showed significantly higher blood perfusion around the defect and enhanced new bone formation around acellular bone graft material.The results implicate that rapid angiogenesis in the initial healing period by PRP could be supposed as a way to overcome short term effect of the rapid angiogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral & Maxillofacial Surgery, College of Medicine, Chungnam National University, Daejeon, Korea.

ABSTRACT

Purpose: Although most researchers agree that platelet-rich plasma (PRP) is a good source of autogenous growth factors, its effect on bone regeneration is still controversial. The purpose of this study was to evaluate whether increasing angiogenic factors in the human PRP to enhance new bone formation through rapid angiogenesis.

Material and methods: In vitro, the human platelets were activated with application of shear stress, 20 µg/ml collagen, 2 mM CaCl(2) and 10U thrombin/1 × 10(9) platelets. Level of vascular endothelial growth factor (VEGF) and platelet microparticle (PMP) in the activated platelets were checked. In the animal study, human angiogenic factors-enriched PRP was tested in 28 athymic rat's cranial critical bone defects with β-TCP. Angiogenesis and osteogenesis were evaluated by laser Doppler perfusion imaging, histology, dual energy X-ray densinometry, and micro-computed tomography.

Results: In vitro, this human angiogenic factors-enriched PRP resulted in better cellular proliferation and osteogenic differentiation. In vivo, increasing angiogenic potential of the PRP showed significantly higher blood perfusion around the defect and enhanced new bone formation around acellular bone graft material.

Conclusion: Angiogenic factor-enriched PRP leads to faster and more extensive new bone formation in the critical size bone defect. The results implicate that rapid angiogenesis in the initial healing period by PRP could be supposed as a way to overcome short term effect of the rapid angiogenesis.

No MeSH data available.


Related in: MedlinePlus