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Preventive effects of Flos Perariae (Gehua) water extract and its active ingredient puerarin in rodent alcoholism models.

Zhang Z, Li S, Jiang J, Yu P, Liang J, Wang Y - Chin Med (2010)

Bottom Line: FPE and puerarin pretreatment significantly prolonged the time of LORR induced by diazepam in acute alcoholic rat.Puerarin increased expression of gama-aminobutyric acid type A receptor alpha1 subunit and decreased expression of alpha4 subunit.In chronic alcoholic mice, puerarin pretreatment significantly increased body weight and liver ADH activity in a dose-dependent manner.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of New Drug Research and Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine & New Drug Research, Jinan University College of Pharmacy, Guangzhou 510632, PR China. tlisha@jnu.edu.cn.

ABSTRACT

Background: Radix Puerariae is used in Chinese medicine to treat alcohol addiction and intoxication. The present study investigates the effects of Flos puerariae lobatae water extract (FPE) and its active ingredient puerarin on alcoholism using rodent models.

Methods: Alcoholic animals were given FPE or puerarin by oral intubation prior or after alcohol treatment. The loss of righting reflex (LORR) assay was used to evaluate sedative/hypnotic effects. Changes of gama-aminobutyric acid type A receptor (GABAAR) subunits induced by alcohol treatment in hippocampus were measured with western blot. In alcoholic mice, body weight gain was monitored throughout the experiments. Alcohol dehydrogenase (ADH) levels in liver were measured.

Results: FPE and puerarin pretreatment significantly prolonged the time of LORR induced by diazepam in acute alcoholic rat. Puerarin increased expression of gama-aminobutyric acid type A receptor alpha1 subunit and decreased expression of alpha4 subunit. In chronic alcoholic mice, puerarin pretreatment significantly increased body weight and liver ADH activity in a dose-dependent manner. Puerarin pretreatment, but not post-treatment, can reverse the changes of gama-aminobutyric acid type A receptor subunit expression and increase ADH activity in alcoholism models.

Conclusion: The present study demonstrates that FPE and its active ingredient puerarin have preventive effects on alcoholism related disorders.

No MeSH data available.


Related in: MedlinePlus

Puerarin increases the ADH activity in alcoholic mice. Data are expressed as mean ± SD (n = 8). *P < 0.05 compared with 'alcohol+saline' treated group; **P < 0.01 compared with 'saline+saline' treated group.
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Figure 5: Puerarin increases the ADH activity in alcoholic mice. Data are expressed as mean ± SD (n = 8). *P < 0.05 compared with 'alcohol+saline' treated group; **P < 0.01 compared with 'saline+saline' treated group.

Mentions: ADH activity in the alcoholic mice significantly decreased compared to that in normal mice [P = 0.002]. Puerarin pretreatment reversed this decrease in ADH activity in the livers of alcoholic mice in a dose-dependent manner (Figure 5), suggesting that puerarin may exert its preventive effects in alcoholism through enhancing ADH activity.


Preventive effects of Flos Perariae (Gehua) water extract and its active ingredient puerarin in rodent alcoholism models.

Zhang Z, Li S, Jiang J, Yu P, Liang J, Wang Y - Chin Med (2010)

Puerarin increases the ADH activity in alcoholic mice. Data are expressed as mean ± SD (n = 8). *P < 0.05 compared with 'alcohol+saline' treated group; **P < 0.01 compared with 'saline+saline' treated group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2984509&req=5

Figure 5: Puerarin increases the ADH activity in alcoholic mice. Data are expressed as mean ± SD (n = 8). *P < 0.05 compared with 'alcohol+saline' treated group; **P < 0.01 compared with 'saline+saline' treated group.
Mentions: ADH activity in the alcoholic mice significantly decreased compared to that in normal mice [P = 0.002]. Puerarin pretreatment reversed this decrease in ADH activity in the livers of alcoholic mice in a dose-dependent manner (Figure 5), suggesting that puerarin may exert its preventive effects in alcoholism through enhancing ADH activity.

Bottom Line: FPE and puerarin pretreatment significantly prolonged the time of LORR induced by diazepam in acute alcoholic rat.Puerarin increased expression of gama-aminobutyric acid type A receptor alpha1 subunit and decreased expression of alpha4 subunit.In chronic alcoholic mice, puerarin pretreatment significantly increased body weight and liver ADH activity in a dose-dependent manner.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of New Drug Research and Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine & New Drug Research, Jinan University College of Pharmacy, Guangzhou 510632, PR China. tlisha@jnu.edu.cn.

ABSTRACT

Background: Radix Puerariae is used in Chinese medicine to treat alcohol addiction and intoxication. The present study investigates the effects of Flos puerariae lobatae water extract (FPE) and its active ingredient puerarin on alcoholism using rodent models.

Methods: Alcoholic animals were given FPE or puerarin by oral intubation prior or after alcohol treatment. The loss of righting reflex (LORR) assay was used to evaluate sedative/hypnotic effects. Changes of gama-aminobutyric acid type A receptor (GABAAR) subunits induced by alcohol treatment in hippocampus were measured with western blot. In alcoholic mice, body weight gain was monitored throughout the experiments. Alcohol dehydrogenase (ADH) levels in liver were measured.

Results: FPE and puerarin pretreatment significantly prolonged the time of LORR induced by diazepam in acute alcoholic rat. Puerarin increased expression of gama-aminobutyric acid type A receptor alpha1 subunit and decreased expression of alpha4 subunit. In chronic alcoholic mice, puerarin pretreatment significantly increased body weight and liver ADH activity in a dose-dependent manner. Puerarin pretreatment, but not post-treatment, can reverse the changes of gama-aminobutyric acid type A receptor subunit expression and increase ADH activity in alcoholism models.

Conclusion: The present study demonstrates that FPE and its active ingredient puerarin have preventive effects on alcoholism related disorders.

No MeSH data available.


Related in: MedlinePlus