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Preventive effects of Flos Perariae (Gehua) water extract and its active ingredient puerarin in rodent alcoholism models.

Zhang Z, Li S, Jiang J, Yu P, Liang J, Wang Y - Chin Med (2010)

Bottom Line: FPE and puerarin pretreatment significantly prolonged the time of LORR induced by diazepam in acute alcoholic rat.Puerarin increased expression of gama-aminobutyric acid type A receptor alpha1 subunit and decreased expression of alpha4 subunit.In chronic alcoholic mice, puerarin pretreatment significantly increased body weight and liver ADH activity in a dose-dependent manner.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of New Drug Research and Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine & New Drug Research, Jinan University College of Pharmacy, Guangzhou 510632, PR China. tlisha@jnu.edu.cn.

ABSTRACT

Background: Radix Puerariae is used in Chinese medicine to treat alcohol addiction and intoxication. The present study investigates the effects of Flos puerariae lobatae water extract (FPE) and its active ingredient puerarin on alcoholism using rodent models.

Methods: Alcoholic animals were given FPE or puerarin by oral intubation prior or after alcohol treatment. The loss of righting reflex (LORR) assay was used to evaluate sedative/hypnotic effects. Changes of gama-aminobutyric acid type A receptor (GABAAR) subunits induced by alcohol treatment in hippocampus were measured with western blot. In alcoholic mice, body weight gain was monitored throughout the experiments. Alcohol dehydrogenase (ADH) levels in liver were measured.

Results: FPE and puerarin pretreatment significantly prolonged the time of LORR induced by diazepam in acute alcoholic rat. Puerarin increased expression of gama-aminobutyric acid type A receptor alpha1 subunit and decreased expression of alpha4 subunit. In chronic alcoholic mice, puerarin pretreatment significantly increased body weight and liver ADH activity in a dose-dependent manner. Puerarin pretreatment, but not post-treatment, can reverse the changes of gama-aminobutyric acid type A receptor subunit expression and increase ADH activity in alcoholism models.

Conclusion: The present study demonstrates that FPE and its active ingredient puerarin have preventive effects on alcoholism related disorders.

No MeSH data available.


Related in: MedlinePlus

Representative western blot of protein expression of GABAAR α1 and α4 subunit.
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Figure 3: Representative western blot of protein expression of GABAAR α1 and α4 subunit.

Mentions: Alcohol intoxication significantly decreased GABAAR α1 subunit expression in the hippocampus (Figure 3 and Figure 4A) whereas GABAAR α4 subunit expression was notably increased (Figures 3 and 4B). These results were consistent with those reported previously by Cagetti et al [17]. Puerarin pretreatment reversed the effects on GABAAR subunit expression changes in alcoholic rats. Puerarin treatment after alcohol administration showed less effect than the puerarin pretreatment.


Preventive effects of Flos Perariae (Gehua) water extract and its active ingredient puerarin in rodent alcoholism models.

Zhang Z, Li S, Jiang J, Yu P, Liang J, Wang Y - Chin Med (2010)

Representative western blot of protein expression of GABAAR α1 and α4 subunit.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2984509&req=5

Figure 3: Representative western blot of protein expression of GABAAR α1 and α4 subunit.
Mentions: Alcohol intoxication significantly decreased GABAAR α1 subunit expression in the hippocampus (Figure 3 and Figure 4A) whereas GABAAR α4 subunit expression was notably increased (Figures 3 and 4B). These results were consistent with those reported previously by Cagetti et al [17]. Puerarin pretreatment reversed the effects on GABAAR subunit expression changes in alcoholic rats. Puerarin treatment after alcohol administration showed less effect than the puerarin pretreatment.

Bottom Line: FPE and puerarin pretreatment significantly prolonged the time of LORR induced by diazepam in acute alcoholic rat.Puerarin increased expression of gama-aminobutyric acid type A receptor alpha1 subunit and decreased expression of alpha4 subunit.In chronic alcoholic mice, puerarin pretreatment significantly increased body weight and liver ADH activity in a dose-dependent manner.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of New Drug Research and Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine & New Drug Research, Jinan University College of Pharmacy, Guangzhou 510632, PR China. tlisha@jnu.edu.cn.

ABSTRACT

Background: Radix Puerariae is used in Chinese medicine to treat alcohol addiction and intoxication. The present study investigates the effects of Flos puerariae lobatae water extract (FPE) and its active ingredient puerarin on alcoholism using rodent models.

Methods: Alcoholic animals were given FPE or puerarin by oral intubation prior or after alcohol treatment. The loss of righting reflex (LORR) assay was used to evaluate sedative/hypnotic effects. Changes of gama-aminobutyric acid type A receptor (GABAAR) subunits induced by alcohol treatment in hippocampus were measured with western blot. In alcoholic mice, body weight gain was monitored throughout the experiments. Alcohol dehydrogenase (ADH) levels in liver were measured.

Results: FPE and puerarin pretreatment significantly prolonged the time of LORR induced by diazepam in acute alcoholic rat. Puerarin increased expression of gama-aminobutyric acid type A receptor alpha1 subunit and decreased expression of alpha4 subunit. In chronic alcoholic mice, puerarin pretreatment significantly increased body weight and liver ADH activity in a dose-dependent manner. Puerarin pretreatment, but not post-treatment, can reverse the changes of gama-aminobutyric acid type A receptor subunit expression and increase ADH activity in alcoholism models.

Conclusion: The present study demonstrates that FPE and its active ingredient puerarin have preventive effects on alcoholism related disorders.

No MeSH data available.


Related in: MedlinePlus