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Preventive effects of Flos Perariae (Gehua) water extract and its active ingredient puerarin in rodent alcoholism models.

Zhang Z, Li S, Jiang J, Yu P, Liang J, Wang Y - Chin Med (2010)

Bottom Line: FPE and puerarin pretreatment significantly prolonged the time of LORR induced by diazepam in acute alcoholic rat.Puerarin increased expression of gama-aminobutyric acid type A receptor alpha1 subunit and decreased expression of alpha4 subunit.In chronic alcoholic mice, puerarin pretreatment significantly increased body weight and liver ADH activity in a dose-dependent manner.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of New Drug Research and Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine & New Drug Research, Jinan University College of Pharmacy, Guangzhou 510632, PR China. tlisha@jnu.edu.cn.

ABSTRACT

Background: Radix Puerariae is used in Chinese medicine to treat alcohol addiction and intoxication. The present study investigates the effects of Flos puerariae lobatae water extract (FPE) and its active ingredient puerarin on alcoholism using rodent models.

Methods: Alcoholic animals were given FPE or puerarin by oral intubation prior or after alcohol treatment. The loss of righting reflex (LORR) assay was used to evaluate sedative/hypnotic effects. Changes of gama-aminobutyric acid type A receptor (GABAAR) subunits induced by alcohol treatment in hippocampus were measured with western blot. In alcoholic mice, body weight gain was monitored throughout the experiments. Alcohol dehydrogenase (ADH) levels in liver were measured.

Results: FPE and puerarin pretreatment significantly prolonged the time of LORR induced by diazepam in acute alcoholic rat. Puerarin increased expression of gama-aminobutyric acid type A receptor alpha1 subunit and decreased expression of alpha4 subunit. In chronic alcoholic mice, puerarin pretreatment significantly increased body weight and liver ADH activity in a dose-dependent manner. Puerarin pretreatment, but not post-treatment, can reverse the changes of gama-aminobutyric acid type A receptor subunit expression and increase ADH activity in alcoholism models.

Conclusion: The present study demonstrates that FPE and its active ingredient puerarin have preventive effects on alcoholism related disorders.

No MeSH data available.


Related in: MedlinePlus

Effects of FPE (A) and puerarin (B) on duration of diazepam induced LORR in normal and alcoholic rats. Data are expressed as mean ± SD (n = 6). *P < 0.05 compared with 'alcohol+saline' treated group; **P < 0.01 compared with 'saline+saline' treated group.
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Figure 2: Effects of FPE (A) and puerarin (B) on duration of diazepam induced LORR in normal and alcoholic rats. Data are expressed as mean ± SD (n = 6). *P < 0.05 compared with 'alcohol+saline' treated group; **P < 0.01 compared with 'saline+saline' treated group.

Mentions: As shown in Figure 2, LORR induced by diazepam significantly decreased [P = 0.0003] in acute alcoholic rats (alcohol + saline group). The duration of diazepam-induced LORR was about 60 minutes in normal rats (saline + saline group, Figure 2); however, LORR of the acute alcoholic rats was at 9.8 ± 3.27 minutes (Figure 2) which was significantly different from that of the normal rats [P = 0.0003]. FPE and puerarin alone had no significant effect in the duration of LORR in normal rats. Pretreatment with FPE or puerarin significantly recovered the LORR time of the acute alcoholic rats, which went up to 49 ± 18.64 and 51.83 ± 6.11 minutes respectively, [P = 0.001] against alcohol + saline group). However, FPE or puerarin administration post-alcohol treatment did not significantly recover the duration of LORR (Figure 2).


Preventive effects of Flos Perariae (Gehua) water extract and its active ingredient puerarin in rodent alcoholism models.

Zhang Z, Li S, Jiang J, Yu P, Liang J, Wang Y - Chin Med (2010)

Effects of FPE (A) and puerarin (B) on duration of diazepam induced LORR in normal and alcoholic rats. Data are expressed as mean ± SD (n = 6). *P < 0.05 compared with 'alcohol+saline' treated group; **P < 0.01 compared with 'saline+saline' treated group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2984509&req=5

Figure 2: Effects of FPE (A) and puerarin (B) on duration of diazepam induced LORR in normal and alcoholic rats. Data are expressed as mean ± SD (n = 6). *P < 0.05 compared with 'alcohol+saline' treated group; **P < 0.01 compared with 'saline+saline' treated group.
Mentions: As shown in Figure 2, LORR induced by diazepam significantly decreased [P = 0.0003] in acute alcoholic rats (alcohol + saline group). The duration of diazepam-induced LORR was about 60 minutes in normal rats (saline + saline group, Figure 2); however, LORR of the acute alcoholic rats was at 9.8 ± 3.27 minutes (Figure 2) which was significantly different from that of the normal rats [P = 0.0003]. FPE and puerarin alone had no significant effect in the duration of LORR in normal rats. Pretreatment with FPE or puerarin significantly recovered the LORR time of the acute alcoholic rats, which went up to 49 ± 18.64 and 51.83 ± 6.11 minutes respectively, [P = 0.001] against alcohol + saline group). However, FPE or puerarin administration post-alcohol treatment did not significantly recover the duration of LORR (Figure 2).

Bottom Line: FPE and puerarin pretreatment significantly prolonged the time of LORR induced by diazepam in acute alcoholic rat.Puerarin increased expression of gama-aminobutyric acid type A receptor alpha1 subunit and decreased expression of alpha4 subunit.In chronic alcoholic mice, puerarin pretreatment significantly increased body weight and liver ADH activity in a dose-dependent manner.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of New Drug Research and Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine & New Drug Research, Jinan University College of Pharmacy, Guangzhou 510632, PR China. tlisha@jnu.edu.cn.

ABSTRACT

Background: Radix Puerariae is used in Chinese medicine to treat alcohol addiction and intoxication. The present study investigates the effects of Flos puerariae lobatae water extract (FPE) and its active ingredient puerarin on alcoholism using rodent models.

Methods: Alcoholic animals were given FPE or puerarin by oral intubation prior or after alcohol treatment. The loss of righting reflex (LORR) assay was used to evaluate sedative/hypnotic effects. Changes of gama-aminobutyric acid type A receptor (GABAAR) subunits induced by alcohol treatment in hippocampus were measured with western blot. In alcoholic mice, body weight gain was monitored throughout the experiments. Alcohol dehydrogenase (ADH) levels in liver were measured.

Results: FPE and puerarin pretreatment significantly prolonged the time of LORR induced by diazepam in acute alcoholic rat. Puerarin increased expression of gama-aminobutyric acid type A receptor alpha1 subunit and decreased expression of alpha4 subunit. In chronic alcoholic mice, puerarin pretreatment significantly increased body weight and liver ADH activity in a dose-dependent manner. Puerarin pretreatment, but not post-treatment, can reverse the changes of gama-aminobutyric acid type A receptor subunit expression and increase ADH activity in alcoholism models.

Conclusion: The present study demonstrates that FPE and its active ingredient puerarin have preventive effects on alcoholism related disorders.

No MeSH data available.


Related in: MedlinePlus