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Protective effects and potential mechanisms of Pien Tze Huang on cerebral chronic ischemia and hypertensive stroke.

Zhang L, Lam WP, Lü L, Wang C, Wong YW, Lam LH, Tang HC, Wai MS, Wang M, Kwong WH, Ngai SM, Mak YT, Yew DT - Chin Med (2010)

Bottom Line: Pien Tze Huang (PZH), a Chinese medicine formula, was found to have anti-edema, anti-inflammatory and anti-thrombotic effects that can prevent brain damage.Immunostaining of caspase-3 results indicated that Pien Tze Huang prevents brain cells from apoptosis caused by ischemia.The effect of Pien Tze Huang was possibly related to prevention of cell death from apoptosis or ROS/oxidative damage in mitochondria.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China. david-yew@cuhk.edu.hk.

ABSTRACT

Background: Stroke caused by brain ischemia is the third leading cause of adult disability. Active prevention and early treatment of stroke targeting the causes and risk factors may decrease its incidence, mortality and subsequent disability. Pien Tze Huang (PZH), a Chinese medicine formula, was found to have anti-edema, anti-inflammatory and anti-thrombotic effects that can prevent brain damage. This study aims to investigate the potential mechanisms of the preventive effects of Pien Tze Huang on brain damage caused by chronic ischemia and hypertensive stroke in rats.

Methods: The effects of Pien Tze Huang on brain protein expression in spontaneously hypertensive rat (SHR) and stroke prone SHR (SHRsp) were studied with 2-D gel electrophoresis and mass spectrometric analysis with a matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF)/TOF tandem mass spectrometer and on brain cell death with enzyme link immunosorbent assay (ELISA) and immunostaining.

Results: Pien Tze Huang decreased cell death in hippocampus and cerebellum caused by chronic ischemia and hypertensive stroke. Immunostaining of caspase-3 results indicated that Pien Tze Huang prevents brain cells from apoptosis caused by ischemia. Brain protein expression results suggested that Pien Tze Huang downregulated QCR2 in the electron transfer chain of mitochondria preventing reactive oxygen species (ROS) damage and possibly subsequent cell death (caspase 3 assay) as caused by chronic ischemia or hypertensive stroke to hippocampus and cerebellum.

Conclusion: Pien Tze Huang showed preventive effects on limiting the damage or injury caused by chronic ischemia and hypertensive stroke in rats. The effect of Pien Tze Huang was possibly related to prevention of cell death from apoptosis or ROS/oxidative damage in mitochondria.

No MeSH data available.


Related in: MedlinePlus

Cell death assay results in SHRsp. Cell death in hippocampus and cerebellum samples from SHR-sp. Group 5A was fed with daily PZH until the stroke occurred, while Group 5B was given saline. Hippocampus and cerebellum tissues were sampled when the rats died after stroke. SHRsp treated with PZH (Group 5A)  before stroke showed significantly less (* P < 0.05) cell death as compared to those rats without PZH treatment (Group 5B) in both hippocampus and cerebellum.
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Figure 2: Cell death assay results in SHRsp. Cell death in hippocampus and cerebellum samples from SHR-sp. Group 5A was fed with daily PZH until the stroke occurred, while Group 5B was given saline. Hippocampus and cerebellum tissues were sampled when the rats died after stroke. SHRsp treated with PZH (Group 5A) before stroke showed significantly less (* P < 0.05) cell death as compared to those rats without PZH treatment (Group 5B) in both hippocampus and cerebellum.

Mentions: The hippocampus and cerebellum samples obtained from the SHRsp showed that there were significantly less cell deaths in the PZH treated group (Group 5A) when compared to the control (Group 5B) in the hippocampus (Group 5A: 1.17 ± 0.44; Group 5B: 2.16 ± 0.54; P < 0.001) and cerebellum (0.63 ± 0.30; 0.92 ± 0.37; P = 0.018) (Figure 2), suggesting that PZH preventive treatment played a significant protective role against cell deaths caused by stroke in the hippocampus and cerebellum.


Protective effects and potential mechanisms of Pien Tze Huang on cerebral chronic ischemia and hypertensive stroke.

Zhang L, Lam WP, Lü L, Wang C, Wong YW, Lam LH, Tang HC, Wai MS, Wang M, Kwong WH, Ngai SM, Mak YT, Yew DT - Chin Med (2010)

Cell death assay results in SHRsp. Cell death in hippocampus and cerebellum samples from SHR-sp. Group 5A was fed with daily PZH until the stroke occurred, while Group 5B was given saline. Hippocampus and cerebellum tissues were sampled when the rats died after stroke. SHRsp treated with PZH (Group 5A)  before stroke showed significantly less (* P < 0.05) cell death as compared to those rats without PZH treatment (Group 5B) in both hippocampus and cerebellum.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2984508&req=5

Figure 2: Cell death assay results in SHRsp. Cell death in hippocampus and cerebellum samples from SHR-sp. Group 5A was fed with daily PZH until the stroke occurred, while Group 5B was given saline. Hippocampus and cerebellum tissues were sampled when the rats died after stroke. SHRsp treated with PZH (Group 5A) before stroke showed significantly less (* P < 0.05) cell death as compared to those rats without PZH treatment (Group 5B) in both hippocampus and cerebellum.
Mentions: The hippocampus and cerebellum samples obtained from the SHRsp showed that there were significantly less cell deaths in the PZH treated group (Group 5A) when compared to the control (Group 5B) in the hippocampus (Group 5A: 1.17 ± 0.44; Group 5B: 2.16 ± 0.54; P < 0.001) and cerebellum (0.63 ± 0.30; 0.92 ± 0.37; P = 0.018) (Figure 2), suggesting that PZH preventive treatment played a significant protective role against cell deaths caused by stroke in the hippocampus and cerebellum.

Bottom Line: Pien Tze Huang (PZH), a Chinese medicine formula, was found to have anti-edema, anti-inflammatory and anti-thrombotic effects that can prevent brain damage.Immunostaining of caspase-3 results indicated that Pien Tze Huang prevents brain cells from apoptosis caused by ischemia.The effect of Pien Tze Huang was possibly related to prevention of cell death from apoptosis or ROS/oxidative damage in mitochondria.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China. david-yew@cuhk.edu.hk.

ABSTRACT

Background: Stroke caused by brain ischemia is the third leading cause of adult disability. Active prevention and early treatment of stroke targeting the causes and risk factors may decrease its incidence, mortality and subsequent disability. Pien Tze Huang (PZH), a Chinese medicine formula, was found to have anti-edema, anti-inflammatory and anti-thrombotic effects that can prevent brain damage. This study aims to investigate the potential mechanisms of the preventive effects of Pien Tze Huang on brain damage caused by chronic ischemia and hypertensive stroke in rats.

Methods: The effects of Pien Tze Huang on brain protein expression in spontaneously hypertensive rat (SHR) and stroke prone SHR (SHRsp) were studied with 2-D gel electrophoresis and mass spectrometric analysis with a matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF)/TOF tandem mass spectrometer and on brain cell death with enzyme link immunosorbent assay (ELISA) and immunostaining.

Results: Pien Tze Huang decreased cell death in hippocampus and cerebellum caused by chronic ischemia and hypertensive stroke. Immunostaining of caspase-3 results indicated that Pien Tze Huang prevents brain cells from apoptosis caused by ischemia. Brain protein expression results suggested that Pien Tze Huang downregulated QCR2 in the electron transfer chain of mitochondria preventing reactive oxygen species (ROS) damage and possibly subsequent cell death (caspase 3 assay) as caused by chronic ischemia or hypertensive stroke to hippocampus and cerebellum.

Conclusion: Pien Tze Huang showed preventive effects on limiting the damage or injury caused by chronic ischemia and hypertensive stroke in rats. The effect of Pien Tze Huang was possibly related to prevention of cell death from apoptosis or ROS/oxidative damage in mitochondria.

No MeSH data available.


Related in: MedlinePlus