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A comparative analysis shows morphofunctional differences between the rat and mouse melanin-concentrating hormone systems.

Croizier S, Franchi-Bernard G, Colard C, Poncet F, La Roche A, Risold PY - PLoS ONE (2010)

Bottom Line: Sub-populations of neurons producing melanin-concentrating hormone (MCH) are characterized by distinct projection patterns, birthdates and CART/NK3 expression in rat.Evidence for such sub-populations has not been reported in other species.Furthermore, divergences in the distribution of MCH axons were observed, in particular in the ventromedial hypothalamus.

View Article: PubMed Central - PubMed

Affiliation: Faculté de Médecine et de Pharmacie, Université de Franche-Comté, Besançon, France.

ABSTRACT
Sub-populations of neurons producing melanin-concentrating hormone (MCH) are characterized by distinct projection patterns, birthdates and CART/NK3 expression in rat. Evidence for such sub-populations has not been reported in other species. However, given that genetically engineered mouse lines are now commonly used as experimental models, a better characterization of the anatomy and morphofunctionnal organization of MCH system in this species is then necessary. Combining multiple immunohistochemistry experiments with in situ hybridization, tract tracing or BrdU injections, evidence supporting the hypothesis that rat and mouse MCH systems are not identical was obtained: sub-populations of MCH neurons also exist in mouse, but their relative abundance is different. Furthermore, divergences in the distribution of MCH axons were observed, in particular in the ventromedial hypothalamus. These differences suggest that rat and mouse MCH neurons are differentially involved in anatomical networks that control feeding and the sleep/wake cycle.

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Co-expression of MCH/NEI, CART and NK3.(A–D) Photomicrographs to illustrate the co-expression of NK3 receptor and CART peptide by immunohistochemistry as well as the preproMCH (ppMCH) mRNA using in situ hybridization. CART and NK3 were first detected by immunofluorescence (A and B respectively, C). Then, the presence of the ppMCH mRNA in the same cell bodies was verified by in situ hybridization (D). All MCH positive neurons that expressed CART also contained NK3. (E–G) Photomicrographs showing NEI/CART (arrowheads) or NEI (arrows) labeled neurons. Most NEI-positive neurons localized medially to the fornix expressed CART. NEI and CART co-expression was less frequent lateral to the fornix (G). DMH: dorsomedial nucleus hypothalamus, fx: fornix.
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pone-0015471-g002: Co-expression of MCH/NEI, CART and NK3.(A–D) Photomicrographs to illustrate the co-expression of NK3 receptor and CART peptide by immunohistochemistry as well as the preproMCH (ppMCH) mRNA using in situ hybridization. CART and NK3 were first detected by immunofluorescence (A and B respectively, C). Then, the presence of the ppMCH mRNA in the same cell bodies was verified by in situ hybridization (D). All MCH positive neurons that expressed CART also contained NK3. (E–G) Photomicrographs showing NEI/CART (arrowheads) or NEI (arrows) labeled neurons. Most NEI-positive neurons localized medially to the fornix expressed CART. NEI and CART co-expression was less frequent lateral to the fornix (G). DMH: dorsomedial nucleus hypothalamus, fx: fornix.

Mentions: CART is expressed in 66% of all hypothalamic MCH cell bodies in rat hypothalamus [10]. These neurons also express the NK3 receptor [13]. Using a dual immunohistochemical approach on cryostat cut sections, perikarya labeled by the sMCH- or NEI-AS were also labeled by CART monoclonal antibodies (Figure 2E–G). These neurons represented 44.5% of the whole population as compared to 66% in rat (Table 1). By detecting CART, NK3 (immunohistochemistry) and MCH (in situ hybridization) on the same sections, all MCH/CART neurons were found to also express NK3, as in rat (Figure 2A–D). No other CART or NK3 neurons could be observed in the caudal LHA. Most of the rostral MCH neurons in the zona incerta or medial to the fornix expressed CART/NK3, but most of the lateral and caudal MCH neurons did not. A similar segregation of the two sub-populations was reported in the rat [10].


A comparative analysis shows morphofunctional differences between the rat and mouse melanin-concentrating hormone systems.

Croizier S, Franchi-Bernard G, Colard C, Poncet F, La Roche A, Risold PY - PLoS ONE (2010)

Co-expression of MCH/NEI, CART and NK3.(A–D) Photomicrographs to illustrate the co-expression of NK3 receptor and CART peptide by immunohistochemistry as well as the preproMCH (ppMCH) mRNA using in situ hybridization. CART and NK3 were first detected by immunofluorescence (A and B respectively, C). Then, the presence of the ppMCH mRNA in the same cell bodies was verified by in situ hybridization (D). All MCH positive neurons that expressed CART also contained NK3. (E–G) Photomicrographs showing NEI/CART (arrowheads) or NEI (arrows) labeled neurons. Most NEI-positive neurons localized medially to the fornix expressed CART. NEI and CART co-expression was less frequent lateral to the fornix (G). DMH: dorsomedial nucleus hypothalamus, fx: fornix.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC2984507&req=5

pone-0015471-g002: Co-expression of MCH/NEI, CART and NK3.(A–D) Photomicrographs to illustrate the co-expression of NK3 receptor and CART peptide by immunohistochemistry as well as the preproMCH (ppMCH) mRNA using in situ hybridization. CART and NK3 were first detected by immunofluorescence (A and B respectively, C). Then, the presence of the ppMCH mRNA in the same cell bodies was verified by in situ hybridization (D). All MCH positive neurons that expressed CART also contained NK3. (E–G) Photomicrographs showing NEI/CART (arrowheads) or NEI (arrows) labeled neurons. Most NEI-positive neurons localized medially to the fornix expressed CART. NEI and CART co-expression was less frequent lateral to the fornix (G). DMH: dorsomedial nucleus hypothalamus, fx: fornix.
Mentions: CART is expressed in 66% of all hypothalamic MCH cell bodies in rat hypothalamus [10]. These neurons also express the NK3 receptor [13]. Using a dual immunohistochemical approach on cryostat cut sections, perikarya labeled by the sMCH- or NEI-AS were also labeled by CART monoclonal antibodies (Figure 2E–G). These neurons represented 44.5% of the whole population as compared to 66% in rat (Table 1). By detecting CART, NK3 (immunohistochemistry) and MCH (in situ hybridization) on the same sections, all MCH/CART neurons were found to also express NK3, as in rat (Figure 2A–D). No other CART or NK3 neurons could be observed in the caudal LHA. Most of the rostral MCH neurons in the zona incerta or medial to the fornix expressed CART/NK3, but most of the lateral and caudal MCH neurons did not. A similar segregation of the two sub-populations was reported in the rat [10].

Bottom Line: Sub-populations of neurons producing melanin-concentrating hormone (MCH) are characterized by distinct projection patterns, birthdates and CART/NK3 expression in rat.Evidence for such sub-populations has not been reported in other species.Furthermore, divergences in the distribution of MCH axons were observed, in particular in the ventromedial hypothalamus.

View Article: PubMed Central - PubMed

Affiliation: Faculté de Médecine et de Pharmacie, Université de Franche-Comté, Besançon, France.

ABSTRACT
Sub-populations of neurons producing melanin-concentrating hormone (MCH) are characterized by distinct projection patterns, birthdates and CART/NK3 expression in rat. Evidence for such sub-populations has not been reported in other species. However, given that genetically engineered mouse lines are now commonly used as experimental models, a better characterization of the anatomy and morphofunctionnal organization of MCH system in this species is then necessary. Combining multiple immunohistochemistry experiments with in situ hybridization, tract tracing or BrdU injections, evidence supporting the hypothesis that rat and mouse MCH systems are not identical was obtained: sub-populations of MCH neurons also exist in mouse, but their relative abundance is different. Furthermore, divergences in the distribution of MCH axons were observed, in particular in the ventromedial hypothalamus. These differences suggest that rat and mouse MCH neurons are differentially involved in anatomical networks that control feeding and the sleep/wake cycle.

Show MeSH