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Coexpression of adrenomedullin and its receptor component proteins in the reproductive system of the rat during gestation.

Li L, Tang F, O WS - Reprod. Biol. Endocrinol. (2010)

Bottom Line: Adrenomedullin (ADM), a novel vasorelaxant peptide, was found in human/rat ovaries and uteri.The day on which vaginal sperm were found was taken to be pregnancy day 1.The gene expression of Adm and it receptor components and intense immunostaining of ADM were also found in the oviduct during pregnancy.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Physiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.

ABSTRACT

Background: Adrenomedullin (ADM), a novel vasorelaxant peptide, was found in human/rat ovaries and uteri. Plasma ADM level increases in pregnant women and pregnant rats.

Methods: The gene expression levels of Adm and its receptor components - Crlr, Ramp1, Ramp2 and Ramp3, the ADM peptide concentration and localization in the rat female reproductive system during gestation were studied by real-time RT-PCR, EIA and immunohistochemical techniques.

Results: The mRNAs of Adm and its receptor component and ADM were differentially distributed between implantation sites and inter-implantation sites of the pregnant uterus. The day on which vaginal sperm were found was taken to be pregnancy day 1. The Adm mRNA levels in the implantation sites of the uteri in mid- (day 12) and late pregnancy (day 17) were more than 10-fold higher than those in nonpregnancy, pre-implantation (day 3) or early (day 7) pregnancy. ADM was localized in the endometrial stroma with increased immunoreactivity from nonpregnancy to pregnancy. The ADM level and the mRNA levels of Adm, Crlr, Ramp2 and Ramp3 in the corpus luteum all increased in late pregnancy compared with early pregnancy. The gene expression of Adm and it receptor components and intense immunostaining of ADM were also found in the oviduct during pregnancy.

Conclusions: The gene expressions levels of Adm and its receptor components - Crlr, Ramp1, Ramp2 and Ramp3, and ADM peptide concentration exhibited a spatio-temporal pattern in the rat female reproductive system during gestation and this suggests that ADM may play important roles in gestation.

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Immunocytochemical study of ADM in rat ovary and oviduct. Immunocytochemical study of ADM in the ovary (A, B, C) and oviduct (D) showing positive ADM immunostaining in the follicles (A) and corpora lutea (B). Intense ADM immunostaining was found at the centre of some corpora lutea from late pregnant rats (C) and oviduct (D).
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Figure 7: Immunocytochemical study of ADM in rat ovary and oviduct. Immunocytochemical study of ADM in the ovary (A, B, C) and oviduct (D) showing positive ADM immunostaining in the follicles (A) and corpora lutea (B). Intense ADM immunostaining was found at the centre of some corpora lutea from late pregnant rats (C) and oviduct (D).

Mentions: In the ovary from the nonpregnant rats, ADM positive staining was localized to interstitial cells, ovum, cumulus oophorus and granulose cells of the follicles (Figure 7A) and luteal cells of the corpus luteum (Figure 7B). In the pregnant rats, the majority of the ovarian volume was taken up by the growing corpora lutea, which were also the main location of ADM staining. There was no obvious difference in the density of ADM staining in the corpus luteum from nonpregnant rats to pregnant rats (data not shown), though there was heavy ADM staining at the centre of some corpora lutea from late pregnant rats (Figure 7C).


Coexpression of adrenomedullin and its receptor component proteins in the reproductive system of the rat during gestation.

Li L, Tang F, O WS - Reprod. Biol. Endocrinol. (2010)

Immunocytochemical study of ADM in rat ovary and oviduct. Immunocytochemical study of ADM in the ovary (A, B, C) and oviduct (D) showing positive ADM immunostaining in the follicles (A) and corpora lutea (B). Intense ADM immunostaining was found at the centre of some corpora lutea from late pregnant rats (C) and oviduct (D).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2984462&req=5

Figure 7: Immunocytochemical study of ADM in rat ovary and oviduct. Immunocytochemical study of ADM in the ovary (A, B, C) and oviduct (D) showing positive ADM immunostaining in the follicles (A) and corpora lutea (B). Intense ADM immunostaining was found at the centre of some corpora lutea from late pregnant rats (C) and oviduct (D).
Mentions: In the ovary from the nonpregnant rats, ADM positive staining was localized to interstitial cells, ovum, cumulus oophorus and granulose cells of the follicles (Figure 7A) and luteal cells of the corpus luteum (Figure 7B). In the pregnant rats, the majority of the ovarian volume was taken up by the growing corpora lutea, which were also the main location of ADM staining. There was no obvious difference in the density of ADM staining in the corpus luteum from nonpregnant rats to pregnant rats (data not shown), though there was heavy ADM staining at the centre of some corpora lutea from late pregnant rats (Figure 7C).

Bottom Line: Adrenomedullin (ADM), a novel vasorelaxant peptide, was found in human/rat ovaries and uteri.The day on which vaginal sperm were found was taken to be pregnancy day 1.The gene expression of Adm and it receptor components and intense immunostaining of ADM were also found in the oviduct during pregnancy.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Physiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.

ABSTRACT

Background: Adrenomedullin (ADM), a novel vasorelaxant peptide, was found in human/rat ovaries and uteri. Plasma ADM level increases in pregnant women and pregnant rats.

Methods: The gene expression levels of Adm and its receptor components - Crlr, Ramp1, Ramp2 and Ramp3, the ADM peptide concentration and localization in the rat female reproductive system during gestation were studied by real-time RT-PCR, EIA and immunohistochemical techniques.

Results: The mRNAs of Adm and its receptor component and ADM were differentially distributed between implantation sites and inter-implantation sites of the pregnant uterus. The day on which vaginal sperm were found was taken to be pregnancy day 1. The Adm mRNA levels in the implantation sites of the uteri in mid- (day 12) and late pregnancy (day 17) were more than 10-fold higher than those in nonpregnancy, pre-implantation (day 3) or early (day 7) pregnancy. ADM was localized in the endometrial stroma with increased immunoreactivity from nonpregnancy to pregnancy. The ADM level and the mRNA levels of Adm, Crlr, Ramp2 and Ramp3 in the corpus luteum all increased in late pregnancy compared with early pregnancy. The gene expression of Adm and it receptor components and intense immunostaining of ADM were also found in the oviduct during pregnancy.

Conclusions: The gene expressions levels of Adm and its receptor components - Crlr, Ramp1, Ramp2 and Ramp3, and ADM peptide concentration exhibited a spatio-temporal pattern in the rat female reproductive system during gestation and this suggests that ADM may play important roles in gestation.

Show MeSH
Related in: MedlinePlus