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Coexpression of adrenomedullin and its receptor component proteins in the reproductive system of the rat during gestation.

Li L, Tang F, O WS - Reprod. Biol. Endocrinol. (2010)

Bottom Line: Adrenomedullin (ADM), a novel vasorelaxant peptide, was found in human/rat ovaries and uteri.The day on which vaginal sperm were found was taken to be pregnancy day 1.The gene expression of Adm and it receptor components and intense immunostaining of ADM were also found in the oviduct during pregnancy.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Physiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.

ABSTRACT

Background: Adrenomedullin (ADM), a novel vasorelaxant peptide, was found in human/rat ovaries and uteri. Plasma ADM level increases in pregnant women and pregnant rats.

Methods: The gene expression levels of Adm and its receptor components - Crlr, Ramp1, Ramp2 and Ramp3, the ADM peptide concentration and localization in the rat female reproductive system during gestation were studied by real-time RT-PCR, EIA and immunohistochemical techniques.

Results: The mRNAs of Adm and its receptor component and ADM were differentially distributed between implantation sites and inter-implantation sites of the pregnant uterus. The day on which vaginal sperm were found was taken to be pregnancy day 1. The Adm mRNA levels in the implantation sites of the uteri in mid- (day 12) and late pregnancy (day 17) were more than 10-fold higher than those in nonpregnancy, pre-implantation (day 3) or early (day 7) pregnancy. ADM was localized in the endometrial stroma with increased immunoreactivity from nonpregnancy to pregnancy. The ADM level and the mRNA levels of Adm, Crlr, Ramp2 and Ramp3 in the corpus luteum all increased in late pregnancy compared with early pregnancy. The gene expression of Adm and it receptor components and intense immunostaining of ADM were also found in the oviduct during pregnancy.

Conclusions: The gene expressions levels of Adm and its receptor components - Crlr, Ramp1, Ramp2 and Ramp3, and ADM peptide concentration exhibited a spatio-temporal pattern in the rat female reproductive system during gestation and this suggests that ADM may play important roles in gestation.

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Expression of Adm and its receptor components in the oviduct from early, mid- and late pregnant rat. Expression of Adm, Crlr, Ramp1, Ramp2 and Ramp3 in the oviduct from nonpregnant rats and rats of different pregnancy stages. Data were presented as mean ± SEM. n = 6-7.
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Figure 4: Expression of Adm and its receptor components in the oviduct from early, mid- and late pregnant rat. Expression of Adm, Crlr, Ramp1, Ramp2 and Ramp3 in the oviduct from nonpregnant rats and rats of different pregnancy stages. Data were presented as mean ± SEM. n = 6-7.

Mentions: In the oviduct (Figure 4), the expression levels of Adm decreased from pre-implantation to late pregnancy. The expression levels of Crlr were much lower from early to late gestation than pre-implantation. There was no significant difference in the expression levels of Ramp1, Ramp2 or Ramp3.


Coexpression of adrenomedullin and its receptor component proteins in the reproductive system of the rat during gestation.

Li L, Tang F, O WS - Reprod. Biol. Endocrinol. (2010)

Expression of Adm and its receptor components in the oviduct from early, mid- and late pregnant rat. Expression of Adm, Crlr, Ramp1, Ramp2 and Ramp3 in the oviduct from nonpregnant rats and rats of different pregnancy stages. Data were presented as mean ± SEM. n = 6-7.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2984462&req=5

Figure 4: Expression of Adm and its receptor components in the oviduct from early, mid- and late pregnant rat. Expression of Adm, Crlr, Ramp1, Ramp2 and Ramp3 in the oviduct from nonpregnant rats and rats of different pregnancy stages. Data were presented as mean ± SEM. n = 6-7.
Mentions: In the oviduct (Figure 4), the expression levels of Adm decreased from pre-implantation to late pregnancy. The expression levels of Crlr were much lower from early to late gestation than pre-implantation. There was no significant difference in the expression levels of Ramp1, Ramp2 or Ramp3.

Bottom Line: Adrenomedullin (ADM), a novel vasorelaxant peptide, was found in human/rat ovaries and uteri.The day on which vaginal sperm were found was taken to be pregnancy day 1.The gene expression of Adm and it receptor components and intense immunostaining of ADM were also found in the oviduct during pregnancy.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Physiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.

ABSTRACT

Background: Adrenomedullin (ADM), a novel vasorelaxant peptide, was found in human/rat ovaries and uteri. Plasma ADM level increases in pregnant women and pregnant rats.

Methods: The gene expression levels of Adm and its receptor components - Crlr, Ramp1, Ramp2 and Ramp3, the ADM peptide concentration and localization in the rat female reproductive system during gestation were studied by real-time RT-PCR, EIA and immunohistochemical techniques.

Results: The mRNAs of Adm and its receptor component and ADM were differentially distributed between implantation sites and inter-implantation sites of the pregnant uterus. The day on which vaginal sperm were found was taken to be pregnancy day 1. The Adm mRNA levels in the implantation sites of the uteri in mid- (day 12) and late pregnancy (day 17) were more than 10-fold higher than those in nonpregnancy, pre-implantation (day 3) or early (day 7) pregnancy. ADM was localized in the endometrial stroma with increased immunoreactivity from nonpregnancy to pregnancy. The ADM level and the mRNA levels of Adm, Crlr, Ramp2 and Ramp3 in the corpus luteum all increased in late pregnancy compared with early pregnancy. The gene expression of Adm and it receptor components and intense immunostaining of ADM were also found in the oviduct during pregnancy.

Conclusions: The gene expressions levels of Adm and its receptor components - Crlr, Ramp1, Ramp2 and Ramp3, and ADM peptide concentration exhibited a spatio-temporal pattern in the rat female reproductive system during gestation and this suggests that ADM may play important roles in gestation.

Show MeSH
Related in: MedlinePlus