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Differentiation analyses of adult suspension mononucleated peripheral blood cells of Mus musculus.

Ariffin SH, Abidin IZ, Yazid MD, Wahab RM - Cell Commun. Signal (2010)

Bottom Line: Differentiation into osteoblast cells was concomitant with the activation of the Opn gene, increment of alkaline phosphatase (ALP) activity and the existence of bone nodules, whereas osteoclast cells activated the Catk gene, increment of tartrate resistant acid phosphatase (TRAP) activity and showed resorption activities via resorption pits.Morphology analyses showed the morphology of osteoblast and osteoclast cells after von Kossa and May-Grunwald-Giemsa staining respectively.In conclusion, suspension mononucleated cells have the potentiality to differentiate into mature osteoblasts and osteoclasts, and hence can be categorized as multipotent stem cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia. shahroy8@gmail.com.

ABSTRACT

Background: The purpose of this study is to determine whether isolated suspension mouse peripheral mononucleated blood cells have the potential to differentiate into two distinct types of cells, i.e., osteoblasts and osteoclasts.

Results: Differentiation into osteoblast cells was concomitant with the activation of the Opn gene, increment of alkaline phosphatase (ALP) activity and the existence of bone nodules, whereas osteoclast cells activated the Catk gene, increment of tartrate resistant acid phosphatase (TRAP) activity and showed resorption activities via resorption pits. Morphology analyses showed the morphology of osteoblast and osteoclast cells after von Kossa and May-Grunwald-Giemsa staining respectively.

Conclusions: In conclusion, suspension mononucleated cells have the potentiality to differentiate into mature osteoblasts and osteoclasts, and hence can be categorized as multipotent stem cells.

No MeSH data available.


Related in: MedlinePlus

Morphology analysis of osteoblast differentiation. Von Kossa staining showed that 62% and 98% of mononucleated cells have been differentiated into osteoblast cells after cultured in osteoblast differentiation medium for 7 and 14 days, respectively.
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Figure 7: Morphology analysis of osteoblast differentiation. Von Kossa staining showed that 62% and 98% of mononucleated cells have been differentiated into osteoblast cells after cultured in osteoblast differentiation medium for 7 and 14 days, respectively.

Mentions: During differentiation, not all cells are differentiated into osteoblast and osteoclast cells. After 14 days of osteoblast differentiation, there were only about 98% of mononucleated cells are differentiated into osteoblast (Figure 7), while only 32% of mononucleated cells are differentiated into osteoclast cells after 10 days of osteoclast differentiation (Figure 8).


Differentiation analyses of adult suspension mononucleated peripheral blood cells of Mus musculus.

Ariffin SH, Abidin IZ, Yazid MD, Wahab RM - Cell Commun. Signal (2010)

Morphology analysis of osteoblast differentiation. Von Kossa staining showed that 62% and 98% of mononucleated cells have been differentiated into osteoblast cells after cultured in osteoblast differentiation medium for 7 and 14 days, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2984458&req=5

Figure 7: Morphology analysis of osteoblast differentiation. Von Kossa staining showed that 62% and 98% of mononucleated cells have been differentiated into osteoblast cells after cultured in osteoblast differentiation medium for 7 and 14 days, respectively.
Mentions: During differentiation, not all cells are differentiated into osteoblast and osteoclast cells. After 14 days of osteoblast differentiation, there were only about 98% of mononucleated cells are differentiated into osteoblast (Figure 7), while only 32% of mononucleated cells are differentiated into osteoclast cells after 10 days of osteoclast differentiation (Figure 8).

Bottom Line: Differentiation into osteoblast cells was concomitant with the activation of the Opn gene, increment of alkaline phosphatase (ALP) activity and the existence of bone nodules, whereas osteoclast cells activated the Catk gene, increment of tartrate resistant acid phosphatase (TRAP) activity and showed resorption activities via resorption pits.Morphology analyses showed the morphology of osteoblast and osteoclast cells after von Kossa and May-Grunwald-Giemsa staining respectively.In conclusion, suspension mononucleated cells have the potentiality to differentiate into mature osteoblasts and osteoclasts, and hence can be categorized as multipotent stem cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia. shahroy8@gmail.com.

ABSTRACT

Background: The purpose of this study is to determine whether isolated suspension mouse peripheral mononucleated blood cells have the potential to differentiate into two distinct types of cells, i.e., osteoblasts and osteoclasts.

Results: Differentiation into osteoblast cells was concomitant with the activation of the Opn gene, increment of alkaline phosphatase (ALP) activity and the existence of bone nodules, whereas osteoclast cells activated the Catk gene, increment of tartrate resistant acid phosphatase (TRAP) activity and showed resorption activities via resorption pits. Morphology analyses showed the morphology of osteoblast and osteoclast cells after von Kossa and May-Grunwald-Giemsa staining respectively.

Conclusions: In conclusion, suspension mononucleated cells have the potentiality to differentiate into mature osteoblasts and osteoclasts, and hence can be categorized as multipotent stem cells.

No MeSH data available.


Related in: MedlinePlus