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Differentiation analyses of adult suspension mononucleated peripheral blood cells of Mus musculus.

Ariffin SH, Abidin IZ, Yazid MD, Wahab RM - Cell Commun. Signal (2010)

Bottom Line: Differentiation into osteoblast cells was concomitant with the activation of the Opn gene, increment of alkaline phosphatase (ALP) activity and the existence of bone nodules, whereas osteoclast cells activated the Catk gene, increment of tartrate resistant acid phosphatase (TRAP) activity and showed resorption activities via resorption pits.Morphology analyses showed the morphology of osteoblast and osteoclast cells after von Kossa and May-Grunwald-Giemsa staining respectively.In conclusion, suspension mononucleated cells have the potentiality to differentiate into mature osteoblasts and osteoclasts, and hence can be categorized as multipotent stem cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia. shahroy8@gmail.com.

ABSTRACT

Background: The purpose of this study is to determine whether isolated suspension mouse peripheral mononucleated blood cells have the potential to differentiate into two distinct types of cells, i.e., osteoblasts and osteoclasts.

Results: Differentiation into osteoblast cells was concomitant with the activation of the Opn gene, increment of alkaline phosphatase (ALP) activity and the existence of bone nodules, whereas osteoclast cells activated the Catk gene, increment of tartrate resistant acid phosphatase (TRAP) activity and showed resorption activities via resorption pits. Morphology analyses showed the morphology of osteoblast and osteoclast cells after von Kossa and May-Grunwald-Giemsa staining respectively.

Conclusions: In conclusion, suspension mononucleated cells have the potentiality to differentiate into mature osteoblasts and osteoclasts, and hence can be categorized as multipotent stem cells.

No MeSH data available.


Related in: MedlinePlus

Morphology of mouse mononucleated cells. (A and B) Mononucleated cells cultured in proliferation medium stained by von Kossa and May-Grunwald-Giemsa, respectively. (C) Mononucleated cells after 14 days cultured in osteoblast differentiation medium stained by von Kossa. (D) Mononucleated cells after 10 days cultured in osteoclast differentiation medium stained by May-Grunwald-Giemsa. Black arrows show undifferentiated mononucleated cells. Yellow arrows show the black granules in osteoblast cells. White arrow shows multiple nuclei and gigantic osteoclast cells.
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Figure 6: Morphology of mouse mononucleated cells. (A and B) Mononucleated cells cultured in proliferation medium stained by von Kossa and May-Grunwald-Giemsa, respectively. (C) Mononucleated cells after 14 days cultured in osteoblast differentiation medium stained by von Kossa. (D) Mononucleated cells after 10 days cultured in osteoclast differentiation medium stained by May-Grunwald-Giemsa. Black arrows show undifferentiated mononucleated cells. Yellow arrows show the black granules in osteoblast cells. White arrow shows multiple nuclei and gigantic osteoclast cells.

Mentions: The newly isolated mouse peripheral blood mononucleated cells stained by von Kossa (Figure 6A) and May-Grunwald-Giemsa (Figure 6B) were used as controls in this study. These cells were cultured in proliferation medium without any supplementation of differentiation factors. Morphologically, each cell has a large single nucleus that occupies much of the cytoplasmic space (Figures 6A and 6B). The black arrows show the morphology of mononucleated cells without any minerals and calcium deposited when cultured in the proliferation medium.


Differentiation analyses of adult suspension mononucleated peripheral blood cells of Mus musculus.

Ariffin SH, Abidin IZ, Yazid MD, Wahab RM - Cell Commun. Signal (2010)

Morphology of mouse mononucleated cells. (A and B) Mononucleated cells cultured in proliferation medium stained by von Kossa and May-Grunwald-Giemsa, respectively. (C) Mononucleated cells after 14 days cultured in osteoblast differentiation medium stained by von Kossa. (D) Mononucleated cells after 10 days cultured in osteoclast differentiation medium stained by May-Grunwald-Giemsa. Black arrows show undifferentiated mononucleated cells. Yellow arrows show the black granules in osteoblast cells. White arrow shows multiple nuclei and gigantic osteoclast cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2984458&req=5

Figure 6: Morphology of mouse mononucleated cells. (A and B) Mononucleated cells cultured in proliferation medium stained by von Kossa and May-Grunwald-Giemsa, respectively. (C) Mononucleated cells after 14 days cultured in osteoblast differentiation medium stained by von Kossa. (D) Mononucleated cells after 10 days cultured in osteoclast differentiation medium stained by May-Grunwald-Giemsa. Black arrows show undifferentiated mononucleated cells. Yellow arrows show the black granules in osteoblast cells. White arrow shows multiple nuclei and gigantic osteoclast cells.
Mentions: The newly isolated mouse peripheral blood mononucleated cells stained by von Kossa (Figure 6A) and May-Grunwald-Giemsa (Figure 6B) were used as controls in this study. These cells were cultured in proliferation medium without any supplementation of differentiation factors. Morphologically, each cell has a large single nucleus that occupies much of the cytoplasmic space (Figures 6A and 6B). The black arrows show the morphology of mononucleated cells without any minerals and calcium deposited when cultured in the proliferation medium.

Bottom Line: Differentiation into osteoblast cells was concomitant with the activation of the Opn gene, increment of alkaline phosphatase (ALP) activity and the existence of bone nodules, whereas osteoclast cells activated the Catk gene, increment of tartrate resistant acid phosphatase (TRAP) activity and showed resorption activities via resorption pits.Morphology analyses showed the morphology of osteoblast and osteoclast cells after von Kossa and May-Grunwald-Giemsa staining respectively.In conclusion, suspension mononucleated cells have the potentiality to differentiate into mature osteoblasts and osteoclasts, and hence can be categorized as multipotent stem cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia. shahroy8@gmail.com.

ABSTRACT

Background: The purpose of this study is to determine whether isolated suspension mouse peripheral mononucleated blood cells have the potential to differentiate into two distinct types of cells, i.e., osteoblasts and osteoclasts.

Results: Differentiation into osteoblast cells was concomitant with the activation of the Opn gene, increment of alkaline phosphatase (ALP) activity and the existence of bone nodules, whereas osteoclast cells activated the Catk gene, increment of tartrate resistant acid phosphatase (TRAP) activity and showed resorption activities via resorption pits. Morphology analyses showed the morphology of osteoblast and osteoclast cells after von Kossa and May-Grunwald-Giemsa staining respectively.

Conclusions: In conclusion, suspension mononucleated cells have the potentiality to differentiate into mature osteoblasts and osteoclasts, and hence can be categorized as multipotent stem cells.

No MeSH data available.


Related in: MedlinePlus