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Differentiation analyses of adult suspension mononucleated peripheral blood cells of Mus musculus.

Ariffin SH, Abidin IZ, Yazid MD, Wahab RM - Cell Commun. Signal (2010)

Bottom Line: Differentiation into osteoblast cells was concomitant with the activation of the Opn gene, increment of alkaline phosphatase (ALP) activity and the existence of bone nodules, whereas osteoclast cells activated the Catk gene, increment of tartrate resistant acid phosphatase (TRAP) activity and showed resorption activities via resorption pits.Morphology analyses showed the morphology of osteoblast and osteoclast cells after von Kossa and May-Grunwald-Giemsa staining respectively.In conclusion, suspension mononucleated cells have the potentiality to differentiate into mature osteoblasts and osteoclasts, and hence can be categorized as multipotent stem cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia. shahroy8@gmail.com.

ABSTRACT

Background: The purpose of this study is to determine whether isolated suspension mouse peripheral mononucleated blood cells have the potential to differentiate into two distinct types of cells, i.e., osteoblasts and osteoclasts.

Results: Differentiation into osteoblast cells was concomitant with the activation of the Opn gene, increment of alkaline phosphatase (ALP) activity and the existence of bone nodules, whereas osteoclast cells activated the Catk gene, increment of tartrate resistant acid phosphatase (TRAP) activity and showed resorption activities via resorption pits. Morphology analyses showed the morphology of osteoblast and osteoclast cells after von Kossa and May-Grunwald-Giemsa staining respectively.

Conclusions: In conclusion, suspension mononucleated cells have the potentiality to differentiate into mature osteoblasts and osteoclasts, and hence can be categorized as multipotent stem cells.

No MeSH data available.


Related in: MedlinePlus

Osteoblastic activity on osteologic disc in osteoblast differentiation medium. The discs' background colour became darker each day after von Kossa staining; an indication of the development of bone nodule (B-E) compared to control (A).
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Figure 4: Osteoblastic activity on osteologic disc in osteoblast differentiation medium. The discs' background colour became darker each day after von Kossa staining; an indication of the development of bone nodule (B-E) compared to control (A).

Mentions: Cell activities determination using osteologic discs showed that discs cultured in osteoblast differentiation medium were positive for von Kossa staining, an indication of the existence of calcium nodules (Figure 4). Recent study by Sindrey et al. (1999) showed that generated calcium mineral by osteoblast cells is stained by von Kossa as black or yellow to brown based on the amount of secreted calcium matrices, whereas negative control disc is stained as light brown or gray [24]. The control disc cultured in proliferation medium did not show the formation of calcium nodules on the disc surface (Figure 4A). During differentiation, osteoblasts express the ALP enzyme, which utilizes phosphate from the medium deposits it on the disc surface, which then accumulates to become calcium nodules stained by von Kossa [12,25]. In our study, we compared our discs to the background colour (control disc; cell cultured without osteoblast differentiation factors), where the increment of intensity for the background during cell development has been observed. The background colour and quantity of bone nodules increase starting from day 5 until 14 (Figure 4B-E). Pre-osteoblasts produce high activities after culturing in differentiation medium consisting of ascorbic acid and β-glycerophosphate for 10 and 14 days [14,16].


Differentiation analyses of adult suspension mononucleated peripheral blood cells of Mus musculus.

Ariffin SH, Abidin IZ, Yazid MD, Wahab RM - Cell Commun. Signal (2010)

Osteoblastic activity on osteologic disc in osteoblast differentiation medium. The discs' background colour became darker each day after von Kossa staining; an indication of the development of bone nodule (B-E) compared to control (A).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2984458&req=5

Figure 4: Osteoblastic activity on osteologic disc in osteoblast differentiation medium. The discs' background colour became darker each day after von Kossa staining; an indication of the development of bone nodule (B-E) compared to control (A).
Mentions: Cell activities determination using osteologic discs showed that discs cultured in osteoblast differentiation medium were positive for von Kossa staining, an indication of the existence of calcium nodules (Figure 4). Recent study by Sindrey et al. (1999) showed that generated calcium mineral by osteoblast cells is stained by von Kossa as black or yellow to brown based on the amount of secreted calcium matrices, whereas negative control disc is stained as light brown or gray [24]. The control disc cultured in proliferation medium did not show the formation of calcium nodules on the disc surface (Figure 4A). During differentiation, osteoblasts express the ALP enzyme, which utilizes phosphate from the medium deposits it on the disc surface, which then accumulates to become calcium nodules stained by von Kossa [12,25]. In our study, we compared our discs to the background colour (control disc; cell cultured without osteoblast differentiation factors), where the increment of intensity for the background during cell development has been observed. The background colour and quantity of bone nodules increase starting from day 5 until 14 (Figure 4B-E). Pre-osteoblasts produce high activities after culturing in differentiation medium consisting of ascorbic acid and β-glycerophosphate for 10 and 14 days [14,16].

Bottom Line: Differentiation into osteoblast cells was concomitant with the activation of the Opn gene, increment of alkaline phosphatase (ALP) activity and the existence of bone nodules, whereas osteoclast cells activated the Catk gene, increment of tartrate resistant acid phosphatase (TRAP) activity and showed resorption activities via resorption pits.Morphology analyses showed the morphology of osteoblast and osteoclast cells after von Kossa and May-Grunwald-Giemsa staining respectively.In conclusion, suspension mononucleated cells have the potentiality to differentiate into mature osteoblasts and osteoclasts, and hence can be categorized as multipotent stem cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia. shahroy8@gmail.com.

ABSTRACT

Background: The purpose of this study is to determine whether isolated suspension mouse peripheral mononucleated blood cells have the potential to differentiate into two distinct types of cells, i.e., osteoblasts and osteoclasts.

Results: Differentiation into osteoblast cells was concomitant with the activation of the Opn gene, increment of alkaline phosphatase (ALP) activity and the existence of bone nodules, whereas osteoclast cells activated the Catk gene, increment of tartrate resistant acid phosphatase (TRAP) activity and showed resorption activities via resorption pits. Morphology analyses showed the morphology of osteoblast and osteoclast cells after von Kossa and May-Grunwald-Giemsa staining respectively.

Conclusions: In conclusion, suspension mononucleated cells have the potentiality to differentiate into mature osteoblasts and osteoclasts, and hence can be categorized as multipotent stem cells.

No MeSH data available.


Related in: MedlinePlus