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Differentiation analyses of adult suspension mononucleated peripheral blood cells of Mus musculus.

Ariffin SH, Abidin IZ, Yazid MD, Wahab RM - Cell Commun. Signal (2010)

Bottom Line: Differentiation into osteoblast cells was concomitant with the activation of the Opn gene, increment of alkaline phosphatase (ALP) activity and the existence of bone nodules, whereas osteoclast cells activated the Catk gene, increment of tartrate resistant acid phosphatase (TRAP) activity and showed resorption activities via resorption pits.Morphology analyses showed the morphology of osteoblast and osteoclast cells after von Kossa and May-Grunwald-Giemsa staining respectively.In conclusion, suspension mononucleated cells have the potentiality to differentiate into mature osteoblasts and osteoclasts, and hence can be categorized as multipotent stem cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia. shahroy8@gmail.com.

ABSTRACT

Background: The purpose of this study is to determine whether isolated suspension mouse peripheral mononucleated blood cells have the potential to differentiate into two distinct types of cells, i.e., osteoblasts and osteoclasts.

Results: Differentiation into osteoblast cells was concomitant with the activation of the Opn gene, increment of alkaline phosphatase (ALP) activity and the existence of bone nodules, whereas osteoclast cells activated the Catk gene, increment of tartrate resistant acid phosphatase (TRAP) activity and showed resorption activities via resorption pits. Morphology analyses showed the morphology of osteoblast and osteoclast cells after von Kossa and May-Grunwald-Giemsa staining respectively.

Conclusions: In conclusion, suspension mononucleated cells have the potentiality to differentiate into mature osteoblasts and osteoclasts, and hence can be categorized as multipotent stem cells.

No MeSH data available.


Related in: MedlinePlus

TRAP specific activity for mononucleated cells cultured in osteoclast differentiation medium. TRAP specific activity showed an increment after 10 days mononucleated cells cultured in osteoclast differentiation medium.
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Figure 3: TRAP specific activity for mononucleated cells cultured in osteoclast differentiation medium. TRAP specific activity showed an increment after 10 days mononucleated cells cultured in osteoclast differentiation medium.

Mentions: The experimental results are shown in Figure 1C. The RT-PCR analysis of the transcripts from mononucleated cells cultured in osteoclast differentiation medium showed the activation of the Catk gene. The RT-PCR product showed amplification of a band with the expected size for the Catk gene, i.e., ~350 bp (Figure 1C). However, no band was found from mononucleated cells cultured in proliferation medium, which served as the negative control experiment (Figure 1C). Therefore, activation of the Catk gene does not occur during cells proliferation. This study showed that mononucleated cells cultured in osteoclast differentiation medium for 10 days activate the Catk gene, similar to mature osteoclast cells. In addition, the increment of tartrate resistant acid phosphatase (TRAP) activity in mononucleated cells cultured in osteoclast differentiation medium after 10 days compared to cells cultured in control medium (proliferation medium) also indicates that the cells are differentiated into osteoclast cells (Figure 3).


Differentiation analyses of adult suspension mononucleated peripheral blood cells of Mus musculus.

Ariffin SH, Abidin IZ, Yazid MD, Wahab RM - Cell Commun. Signal (2010)

TRAP specific activity for mononucleated cells cultured in osteoclast differentiation medium. TRAP specific activity showed an increment after 10 days mononucleated cells cultured in osteoclast differentiation medium.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2984458&req=5

Figure 3: TRAP specific activity for mononucleated cells cultured in osteoclast differentiation medium. TRAP specific activity showed an increment after 10 days mononucleated cells cultured in osteoclast differentiation medium.
Mentions: The experimental results are shown in Figure 1C. The RT-PCR analysis of the transcripts from mononucleated cells cultured in osteoclast differentiation medium showed the activation of the Catk gene. The RT-PCR product showed amplification of a band with the expected size for the Catk gene, i.e., ~350 bp (Figure 1C). However, no band was found from mononucleated cells cultured in proliferation medium, which served as the negative control experiment (Figure 1C). Therefore, activation of the Catk gene does not occur during cells proliferation. This study showed that mononucleated cells cultured in osteoclast differentiation medium for 10 days activate the Catk gene, similar to mature osteoclast cells. In addition, the increment of tartrate resistant acid phosphatase (TRAP) activity in mononucleated cells cultured in osteoclast differentiation medium after 10 days compared to cells cultured in control medium (proliferation medium) also indicates that the cells are differentiated into osteoclast cells (Figure 3).

Bottom Line: Differentiation into osteoblast cells was concomitant with the activation of the Opn gene, increment of alkaline phosphatase (ALP) activity and the existence of bone nodules, whereas osteoclast cells activated the Catk gene, increment of tartrate resistant acid phosphatase (TRAP) activity and showed resorption activities via resorption pits.Morphology analyses showed the morphology of osteoblast and osteoclast cells after von Kossa and May-Grunwald-Giemsa staining respectively.In conclusion, suspension mononucleated cells have the potentiality to differentiate into mature osteoblasts and osteoclasts, and hence can be categorized as multipotent stem cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia. shahroy8@gmail.com.

ABSTRACT

Background: The purpose of this study is to determine whether isolated suspension mouse peripheral mononucleated blood cells have the potential to differentiate into two distinct types of cells, i.e., osteoblasts and osteoclasts.

Results: Differentiation into osteoblast cells was concomitant with the activation of the Opn gene, increment of alkaline phosphatase (ALP) activity and the existence of bone nodules, whereas osteoclast cells activated the Catk gene, increment of tartrate resistant acid phosphatase (TRAP) activity and showed resorption activities via resorption pits. Morphology analyses showed the morphology of osteoblast and osteoclast cells after von Kossa and May-Grunwald-Giemsa staining respectively.

Conclusions: In conclusion, suspension mononucleated cells have the potentiality to differentiate into mature osteoblasts and osteoclasts, and hence can be categorized as multipotent stem cells.

No MeSH data available.


Related in: MedlinePlus