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MicroRNA-17-92 significantly enhances radioresistance in human mantle cell lymphoma cells.

Jiang P, Rao EY, Meng N, Zhao Y, Wang JJ - Radiat Oncol (2010)

Bottom Line: The microRNA-17-92 (miRNA-17-92) cluster, at chromosome 13q31-q32, also known as oncomir-1, consists of seven miRNAs that are transcribed as a polycistronic unit.Over-expression of miRNA-17-92 significantly increased survival cell number, cell proliferation and decreased cell death of human MCL cells after different doses of radiation.Immunoblot analysis showed that phosphatase and tension homolog (PTEN) and PHLPP2 was down-modulated and pAkt activity was enhanced in MCL cells after over-expressing miRNA-17-92 after irradiation.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Radiation Oncology, Peking University Third Hospital, Beijing 100191, China.

ABSTRACT
The microRNA-17-92 (miRNA-17-92) cluster, at chromosome 13q31-q32, also known as oncomir-1, consists of seven miRNAs that are transcribed as a polycistronic unit. Over-expression of miRNA-17-92 has been observed in lymphomas and other solid tumors. Whether miRNA-17-92 expression affects the response of tumor cells to radiotherapy is not addressed so far. In the present study, we studied the effects of miRNA-17-92 on the radiosensitivity of human mantle cell lymphoma (MCL) cells Z138c. Over-expression of miRNA-17-92 significantly increased survival cell number, cell proliferation and decreased cell death of human MCL cells after different doses of radiation. Immunoblot analysis showed that phosphatase and tension homolog (PTEN) and PHLPP2 was down-modulated and pAkt activity was enhanced in MCL cells after over-expressing miRNA-17-92 after irradiation. These findings are the first direct evidence that over-expression of miRNA-17-92 cluster significantly increases the radioresistance of human MCL cells, which offers a novel target molecule for improving the radiotherapy of MCL in clinic.

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The cell cycle distribution of Z138c-TMP2 and Z138c-miRNA-17-92 cells after different doses of radiation. The cell cycle of Z138c-TMP2 and Z138c-miRNA-17-92 cells was determined by PI staining and detected by FCM at 1 day after radiation. A) one representative of cell cycle distribution as detected by FCM. B) a summary of different cell phases of Z138c-TMP2 and Z138c-miRNA-17-92 cell lines. Data have been presented as mean ± s.d. (N = 5). One representative of three experiments has been shown. *P < 0.05 as compared among the identical groups.
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Figure 3: The cell cycle distribution of Z138c-TMP2 and Z138c-miRNA-17-92 cells after different doses of radiation. The cell cycle of Z138c-TMP2 and Z138c-miRNA-17-92 cells was determined by PI staining and detected by FCM at 1 day after radiation. A) one representative of cell cycle distribution as detected by FCM. B) a summary of different cell phases of Z138c-TMP2 and Z138c-miRNA-17-92 cell lines. Data have been presented as mean ± s.d. (N = 5). One representative of three experiments has been shown. *P < 0.05 as compared among the identical groups.

Mentions: The cell cycle was determined by PI staining and assayed by FCM. The percentage of G2/M cells in the Z138c-TMP2 cells increased after radiation doses of 2 Gy and 4 Gy comparing with the non-irradiated cells (Figure 3). However, no obvious radiation-induced G2/M cell cycle arrest was observed in Z138c-miRNA-17-92 cells. A statistically significant difference (t = 2.885, P < 0.05) was obtained at a radiation dose of 4 Gy compared between Z138c-TMP2 and Z138c-miRNA-17-92 cells.


MicroRNA-17-92 significantly enhances radioresistance in human mantle cell lymphoma cells.

Jiang P, Rao EY, Meng N, Zhao Y, Wang JJ - Radiat Oncol (2010)

The cell cycle distribution of Z138c-TMP2 and Z138c-miRNA-17-92 cells after different doses of radiation. The cell cycle of Z138c-TMP2 and Z138c-miRNA-17-92 cells was determined by PI staining and detected by FCM at 1 day after radiation. A) one representative of cell cycle distribution as detected by FCM. B) a summary of different cell phases of Z138c-TMP2 and Z138c-miRNA-17-92 cell lines. Data have been presented as mean ± s.d. (N = 5). One representative of three experiments has been shown. *P < 0.05 as compared among the identical groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2984457&req=5

Figure 3: The cell cycle distribution of Z138c-TMP2 and Z138c-miRNA-17-92 cells after different doses of radiation. The cell cycle of Z138c-TMP2 and Z138c-miRNA-17-92 cells was determined by PI staining and detected by FCM at 1 day after radiation. A) one representative of cell cycle distribution as detected by FCM. B) a summary of different cell phases of Z138c-TMP2 and Z138c-miRNA-17-92 cell lines. Data have been presented as mean ± s.d. (N = 5). One representative of three experiments has been shown. *P < 0.05 as compared among the identical groups.
Mentions: The cell cycle was determined by PI staining and assayed by FCM. The percentage of G2/M cells in the Z138c-TMP2 cells increased after radiation doses of 2 Gy and 4 Gy comparing with the non-irradiated cells (Figure 3). However, no obvious radiation-induced G2/M cell cycle arrest was observed in Z138c-miRNA-17-92 cells. A statistically significant difference (t = 2.885, P < 0.05) was obtained at a radiation dose of 4 Gy compared between Z138c-TMP2 and Z138c-miRNA-17-92 cells.

Bottom Line: The microRNA-17-92 (miRNA-17-92) cluster, at chromosome 13q31-q32, also known as oncomir-1, consists of seven miRNAs that are transcribed as a polycistronic unit.Over-expression of miRNA-17-92 significantly increased survival cell number, cell proliferation and decreased cell death of human MCL cells after different doses of radiation.Immunoblot analysis showed that phosphatase and tension homolog (PTEN) and PHLPP2 was down-modulated and pAkt activity was enhanced in MCL cells after over-expressing miRNA-17-92 after irradiation.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Radiation Oncology, Peking University Third Hospital, Beijing 100191, China.

ABSTRACT
The microRNA-17-92 (miRNA-17-92) cluster, at chromosome 13q31-q32, also known as oncomir-1, consists of seven miRNAs that are transcribed as a polycistronic unit. Over-expression of miRNA-17-92 has been observed in lymphomas and other solid tumors. Whether miRNA-17-92 expression affects the response of tumor cells to radiotherapy is not addressed so far. In the present study, we studied the effects of miRNA-17-92 on the radiosensitivity of human mantle cell lymphoma (MCL) cells Z138c. Over-expression of miRNA-17-92 significantly increased survival cell number, cell proliferation and decreased cell death of human MCL cells after different doses of radiation. Immunoblot analysis showed that phosphatase and tension homolog (PTEN) and PHLPP2 was down-modulated and pAkt activity was enhanced in MCL cells after over-expressing miRNA-17-92 after irradiation. These findings are the first direct evidence that over-expression of miRNA-17-92 cluster significantly increases the radioresistance of human MCL cells, which offers a novel target molecule for improving the radiotherapy of MCL in clinic.

Show MeSH
Related in: MedlinePlus