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Image analytic study of nuclear area in mantle cell lymphoma.

Baek T, Huh J, Kwak H, Park M, Lee H - Korean J Hematol (2010)

Bottom Line: The mean nuclear areas of mantle cell lymphoma cells measured by the 3 authors were 37.9 [7.9] µm(2), 37.9 [7.2] µm(2), and 38.2 [7.7] µm(2) and those of normal mantle cells in reactive tonsil were 28.6 [2.3] µm(2), 28.8 [2.0] µm(2), and 27.0 [3.0] µm(2).There was no statistical difference between the 3 observations of mantle cell lymphoma (P=0.580) and normal tonsils.We showed that nuclear area measurement by using image analyzer can be used as an objective quantitative method.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Eulji University Hospital, Daejeon, Korea.

ABSTRACT

Background: Malignant lymphomas are classified on the basis of morphology, immunohistochemistry, and genetic and molecular biological features. Morphology is considered the most important and basic feature. Lymphomas can be classified as small, medium, or large depending on the cell size, but this criterion tends to be rather subjective. The aim of this study was to investigate the usefulness of an objective approach based on quantitative measurements.

Methods: Twenty specimens of mantle cell lymphoma and 2 specimens of the tonsil were examined. The nuclear area of 6,401 tumor cells of mantle cell lymphoma and 743 normal mantle cells of reactive tonsils were measured by 3 authors by using a user-controlled image-analyzer. The images of the nuclei were outlined using the spline method and the i-solution software, and the data were assessed using ANOVA and Student's t-test.

Results: The mean nuclear areas of mantle cell lymphoma cells measured by the 3 authors were 37.9 [7.9] µm(2), 37.9 [7.2] µm(2), and 38.2 [7.7] µm(2) and those of normal mantle cells in reactive tonsil were 28.6 [2.3] µm(2), 28.8 [2.0] µm(2), and 27.0 [3.0] µm(2). There was no statistical difference between the 3 observations of mantle cell lymphoma (P=0.580) and normal tonsils.

Conclusion: For morphology, nuclear area is considered an important feature in the classification schemes of lymphoma. We showed that nuclear area measurement by using image analyzer can be used as an objective quantitative method. We think that nuclear morphometry may play a significant role in the diagnosis of lymphoma.

No MeSH data available.


Related in: MedlinePlus

Distribution of the nuclear area in mantle cell lymphoma. The majority of the values of nuclear area was measured and ranged from 30 to 40 µm2.
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Figure 2: Distribution of the nuclear area in mantle cell lymphoma. The majority of the values of nuclear area was measured and ranged from 30 to 40 µm2.

Mentions: The nuclear area of 6401 MCL cells and 743 normal mantle cell of reactive tonsils were analyzed by 3 pathologists using a user-controlled image analyzer. The first, second, and third pathologist analyzed 2,706, 1,913, and 1,782 MCL cells and 309, 254, and 180 cells of the mantle zone of reactive tonsils, respectively. Thirty red blood cells were randomly selected and analyzed as control group. The mean diameter and mean area of the red blood cells was 6.2 [0.3] µm (average [standard deviation]) and 31.2 [2.0] µm2, respectively. The nuclear areas of MCL cells as measured by the first, second, and third pathologist were 37.9 [7.9] µm2, 37.9 [7.2] µm2, and 38.2 [7.7] µm2, respectively. The nuclear areas of normal mantle cells in reactive tonsils were 28.6 [2.3] µm2, 28.8 [2.0] µm2, and 27.0 [3.0] µm2 as measured by the first, second, and third pathologist, respectively. The distribution of the nuclear area of MCL cells as found by the 3 pathologists is summarized in Table 1 and compared in Fig. 2. Generally, about half of the MCL cells had a nuclear area of 30.0 - 39.9 µm2 and no significant difference was found when comparing the results of the 3 pathologists. The mean value of the inter-observer variation regarding nuclear size was 2.7 [1.7] µm2 (range: 0.04-5.4 µm2). The proportion of the difference of the mean value among the 3 pathologists was as follows. The proportion of cases with a difference in the mean value of less than 1.0 comprised 20%, between 1.0-3.0 comprised 40%, between 3.0-5.0 comprised 25%, and more than 5.0 comprised 15% (Fig. 3). The proportion of difference in the standard deviation of the nuclear size was as follows: less than 1.0 was 30%, 1.0-2.0 was 65%, and more than 2.0 was 15%. The nuclear area of MCL cells was considerably larger than that of mantle cells of reactive tonsils (P<0.001, Student's t-test). The inter-observer variation among the 3 observers showed no significant difference (P=0.580, ANOVA).


Image analytic study of nuclear area in mantle cell lymphoma.

Baek T, Huh J, Kwak H, Park M, Lee H - Korean J Hematol (2010)

Distribution of the nuclear area in mantle cell lymphoma. The majority of the values of nuclear area was measured and ranged from 30 to 40 µm2.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2983036&req=5

Figure 2: Distribution of the nuclear area in mantle cell lymphoma. The majority of the values of nuclear area was measured and ranged from 30 to 40 µm2.
Mentions: The nuclear area of 6401 MCL cells and 743 normal mantle cell of reactive tonsils were analyzed by 3 pathologists using a user-controlled image analyzer. The first, second, and third pathologist analyzed 2,706, 1,913, and 1,782 MCL cells and 309, 254, and 180 cells of the mantle zone of reactive tonsils, respectively. Thirty red blood cells were randomly selected and analyzed as control group. The mean diameter and mean area of the red blood cells was 6.2 [0.3] µm (average [standard deviation]) and 31.2 [2.0] µm2, respectively. The nuclear areas of MCL cells as measured by the first, second, and third pathologist were 37.9 [7.9] µm2, 37.9 [7.2] µm2, and 38.2 [7.7] µm2, respectively. The nuclear areas of normal mantle cells in reactive tonsils were 28.6 [2.3] µm2, 28.8 [2.0] µm2, and 27.0 [3.0] µm2 as measured by the first, second, and third pathologist, respectively. The distribution of the nuclear area of MCL cells as found by the 3 pathologists is summarized in Table 1 and compared in Fig. 2. Generally, about half of the MCL cells had a nuclear area of 30.0 - 39.9 µm2 and no significant difference was found when comparing the results of the 3 pathologists. The mean value of the inter-observer variation regarding nuclear size was 2.7 [1.7] µm2 (range: 0.04-5.4 µm2). The proportion of the difference of the mean value among the 3 pathologists was as follows. The proportion of cases with a difference in the mean value of less than 1.0 comprised 20%, between 1.0-3.0 comprised 40%, between 3.0-5.0 comprised 25%, and more than 5.0 comprised 15% (Fig. 3). The proportion of difference in the standard deviation of the nuclear size was as follows: less than 1.0 was 30%, 1.0-2.0 was 65%, and more than 2.0 was 15%. The nuclear area of MCL cells was considerably larger than that of mantle cells of reactive tonsils (P<0.001, Student's t-test). The inter-observer variation among the 3 observers showed no significant difference (P=0.580, ANOVA).

Bottom Line: The mean nuclear areas of mantle cell lymphoma cells measured by the 3 authors were 37.9 [7.9] µm(2), 37.9 [7.2] µm(2), and 38.2 [7.7] µm(2) and those of normal mantle cells in reactive tonsil were 28.6 [2.3] µm(2), 28.8 [2.0] µm(2), and 27.0 [3.0] µm(2).There was no statistical difference between the 3 observations of mantle cell lymphoma (P=0.580) and normal tonsils.We showed that nuclear area measurement by using image analyzer can be used as an objective quantitative method.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Eulji University Hospital, Daejeon, Korea.

ABSTRACT

Background: Malignant lymphomas are classified on the basis of morphology, immunohistochemistry, and genetic and molecular biological features. Morphology is considered the most important and basic feature. Lymphomas can be classified as small, medium, or large depending on the cell size, but this criterion tends to be rather subjective. The aim of this study was to investigate the usefulness of an objective approach based on quantitative measurements.

Methods: Twenty specimens of mantle cell lymphoma and 2 specimens of the tonsil were examined. The nuclear area of 6,401 tumor cells of mantle cell lymphoma and 743 normal mantle cells of reactive tonsils were measured by 3 authors by using a user-controlled image-analyzer. The images of the nuclei were outlined using the spline method and the i-solution software, and the data were assessed using ANOVA and Student's t-test.

Results: The mean nuclear areas of mantle cell lymphoma cells measured by the 3 authors were 37.9 [7.9] µm(2), 37.9 [7.2] µm(2), and 38.2 [7.7] µm(2) and those of normal mantle cells in reactive tonsil were 28.6 [2.3] µm(2), 28.8 [2.0] µm(2), and 27.0 [3.0] µm(2). There was no statistical difference between the 3 observations of mantle cell lymphoma (P=0.580) and normal tonsils.

Conclusion: For morphology, nuclear area is considered an important feature in the classification schemes of lymphoma. We showed that nuclear area measurement by using image analyzer can be used as an objective quantitative method. We think that nuclear morphometry may play a significant role in the diagnosis of lymphoma.

No MeSH data available.


Related in: MedlinePlus